| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | b3GAT1 |
| Uniprot No | Q9P2W7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-334aa |
| 氨基酸序列 | TLAPLLAVHK DEGSDPRRET PPGADPREYC TSDRDIVEVV RTEYVYTRPP PWSDTLPTIH VVTPTYSRPV QKAELTRMAN TLLHVPNLHW LVVEDAPRRT PLTARLLRDT GLNYTHLHVE TPRNYKLRGD ARDPRIPRGT MQRNLALRWL RETFPRNSSQ PGVVYFADDD NTYSLELFEE MRSTRRVSVW PVAFVGGLRY EAPRVNGAGK VVGWKTVFDP HRPFAIDMAG FAVNLRLILQ RSQAYFKLRG VKGGYQESSL LRELVTLNDL EPKAANCTKI LVWHTRTEKP VLVNEGKKGF TDPSVEI |
| 预测分子量 | 62 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于b3GAT1重组蛋白的3篇代表性文献摘要信息(虚构示例，仅供格式参考)：
1. **文献名称**：Recombinant human β3GAT1 expression and enzymatic characterization
**作者**：Smith J, et al.
**摘要**：研究报道了通过哺乳动物细胞系统成功表达重组人源β3GAT1蛋白，并验证其催化硫酸乙酰肝素糖胺聚糖合成的活性，为糖基化机制研究提供工具。
2. **文献名称**：Structural insights into β3GAT1 function in neural development
**作者**：Li X, Wang Y
**摘要**：通过重组b3GAT1蛋白的晶体结构解析，揭示了其底物结合域的关键氨基酸残基，探讨该酶在神经元迁移中的调控作用。
3. **文献名称**：High-yield production of bioactive β3GAT1 in E. coli
**作者**：Chen R, et al.
**摘要**：开发了一种基于大肠杆菌的重组b3GAT1高效表达及纯化方案，证明其体外修复糖胺聚糖缺陷的潜在治疗应用。
(注：以上文献信息为模拟内容，实际研究中请通过PubMed或专业数据库检索最新文献。)
**Background of b3GAT1 Recombinant Protein**
β3GAT1 (beta-1.3-glucuronyltransferase 1) is a key enzyme involved in the biosynthesis of glycosaminoglycans (GAGs), particularly heparan sulfate and heparin. It catalyzes the transfer of glucuronic acid to the tetrasaccharide linker region during the formation of proteoglycans, which are critical for cell-matrix interactions, signaling, and tissue development. This enzyme is encoded by the *B3GAT1* gene and is primarily localized in the Golgi apparatus.
Recombinant β3GAT1 protein is produced using biotechnological methods, often through expression in mammalian cell systems (e.g., HEK293 or CHO cells) or microbial hosts like *E. coli*, followed by purification to ensure high specificity and activity. Its recombinant form enables detailed study of GAG biosynthesis mechanisms, structure-function relationships, and interactions with other enzymes in the pathway.
Research on β3GAT1 has implications in understanding developmental disorders, cancer metastasis, and neurodegenerative diseases, as aberrant GAG synthesis is linked to these conditions. Additionally, recombinant β3GAT1 serves as a tool for synthesizing defined GAG structures in vitro, aiding drug discovery and the development of therapeutics targeting extracellular matrix pathologies. Its role in modulating cellular adhesion and signaling also makes it relevant for regenerative medicine and biomaterial engineering.
Overall, β3GAT1 recombinant protein bridges fundamental glycobiology research with translational applications, offering insights into disease mechanisms and biotechnological solutions.
×
