| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF4B |
| Uniprot No | P23588 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-611aa |
| 氨基酸序列 | MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGD VSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFL GNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSL LSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWR ARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPCRDMD RYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYED RYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSAS TSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRP RERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENET LNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDT EQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDG GNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDG EDENEGEDYAE |
| 预测分子量 | 93 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF4B重组蛋白的3篇文献概览：
1. **文献名称**: *"Recombinant eIF4B enhances the in vitro translation of mRNAs with structured 5' untranslated regions"*
**作者**: Smith J, et al.
**摘要**: 研究通过大肠杆菌成功表达并纯化重组人源EIF4B蛋白，发现其能显著促进体外翻译系统中含复杂5'非翻译区(如内部核糖体进入位点，IRES)的mRNA的翻译效率，揭示了EIF4B在调控翻译起始中的关键作用。
2. **文献名称**: *"Functional characterization of eIF4B phosphorylation and its role in mTOR signaling"*
**作者**: Zhang Y, et al.
**摘要**: 利用重组EIF4B蛋白进行体外激酶实验，证实mTOR通路通过磷酸化EIF4B的Ser422位点增强其与核糖体及RNA的结合能力，进而促进肿瘤细胞中致癌蛋白的翻译，为靶向EIF4B的抗癌治疗提供了理论依据。
3. **文献名称**: *"Structural insights into eIF4B-RNA interaction by recombinant protein crystallography"*
**作者**: Lee S, et al.
**摘要**: 通过重组表达并结晶EIF4B蛋白的RNA结合域，解析了其与单链RNA结合的分子机制，发现特定结构域通过疏水和氢键相互作用稳定mRNA二级结构的解旋，为开发翻译调控小分子抑制剂提供了结构基础。
(注：以上文献信息为示例性概括，实际文献需根据具体研究内容检索确认。)
**Background of EIF4B Recombinant Protein**
Eukaryotic Initiation Factor 4B (EIF4B) is a critical regulatory protein involved in mRNA translation initiation, a process essential for protein synthesis in eukaryotic cells. It functions as a component of the EIF4F complex, which facilitates the recruitment of ribosomes to mRNA by unwinding secondary structures in the 5' untranslated region (UTR) and promoting circularization of mRNA via interactions with the poly(A)-binding protein (PABP). EIF4B enhances the helicase activity of EIF4A, an RNA helicase, and stabilizes its binding to mRNA, thereby optimizing translation efficiency.
Structurally, EIF4B contains an RNA-binding domain, a region rich in arginine and glycine repeats (RG-rich domain), and sites for phosphorylation by kinases such as AKT, S6K, and MNK. These post-translational modifications regulate its activity, particularly under stress conditions or growth factor stimulation. Dysregulation of EIF4B has been implicated in diseases like cancer, where its overexpression correlates with tumor progression and therapy resistance.
Recombinant EIF4B proteins are engineered using expression systems (e.g., *E. coli*, mammalian cells) to study its molecular interactions, structural dynamics, and role in translational control. These purified proteins enable *in vitro* assays to dissect mechanisms of translation initiation, screen for inhibitors targeting oncogenic signaling pathways, or explore viral strategies that hijack host translation machinery. Additionally, recombinant EIF4B serves as a tool for investigating cellular stress responses, including the formation of stress granules, where translation is transiently halted. Its study provides insights into both basic biology and therapeutic interventions for diseases linked to translational dysregulation.
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