| 纯度 | >95%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DSTN |
| Uniprot No | P60981 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-165aa |
| 氨基酸序列 | MASGVQVADE VCRIFYDMKV RKCSTPEEIK KRKKAVIFCL SADKKCIIVE EGKEILVGDV GVTITDPFKH FVGMLPEKDC RYALYDASFE TKESRKEELM FFLWAPELAP LKSKMIYASS KDAIKKKFQG IKHECQANGP EDLNRACIAE KLGGSLIVAF EGCPVLEHHH HHH |
| 预测分子量 | 20 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DSTN(Destrin/ADF)重组蛋白研究的参考文献示例(注：以下内容为模拟生成，非真实文献)：
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1. **文献名称**: "Recombinant human destrin: cloning, expression, and purification in Escherichia coli"
**作者**: Chen L, et al.
**摘要**: 该研究成功构建了人源DSTN基因的原核表达载体，通过大肠杆菌系统高效表达可溶性重组蛋白。优化纯化工艺后获得高纯度蛋白，并验证其体外促进肌动蛋白丝解聚的活性，为后续功能研究奠定基础。
2. **文献名称**: "Functional characterization of destrin in cancer cell migration using recombinant protein"
**作者**: Tanaka M, et al.
**摘要**: 研究通过昆虫杆状病毒系统表达重组Destrin蛋白，结合细胞迁移实验发现其过表达显著抑制肿瘤细胞的侵袭能力。机制分析表明Destrin通过调控肌动蛋白细胞骨架重塑影响伪足形成。
3. **文献名称**: "Crystal structure of destrin/ADF reveals the molecular basis of actin depolymerization"
**作者**: Wang Y, et al.
**摘要**: 本研究首次解析了重组Destrin蛋白的晶体结构，揭示其与F-actin结合的关键结构域。突变实验证实特定氨基酸残基对肌动蛋白丝的解聚速率具有决定性作用，为开发靶向细胞骨架药物提供理论依据。
4. **文献名称**: "Recombinant destrin as a potential therapeutic agent in neurodegenerative disorders"
**作者**: Johnson R, et al.
**摘要**: 探索重组Destrin蛋白在阿尔茨海默病模型中的应用，发现其通过调节神经元突触的肌动蛋白动态，减轻β-淀粉样蛋白诱导的突触损伤，提示其在神经退行性疾病中的治疗潜力。
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以上示例结合了DSTN重组蛋白的克隆表达、功能机制、结构解析及生物医学应用等方向，符合典型研究脉络。实际文献需通过PubMed/Google Scholar等平台以"recombinant destrin"或"ADF protein expression"为关键词检索。
**Background of DSTN Recombinant Protein**
DSTN, or destrin (also known as actin-depolymerizing factor, ADF), is a conserved eukaryotic protein that regulates actin cytoskeleton dynamics by severing and depolymerizing filamentous actin (F-actin). This activity is critical for cellular processes such as motility, cytokinesis, and intracellular trafficking. Dysregulation of DSTN is implicated in pathological conditions, including cancer metastasis, neurodegenerative disorders, and immune dysfunction, highlighting its biomedical relevance.
Recombinant DSTN proteins are engineered using genetic cloning techniques, where the DSTN gene is expressed in host systems (e.g., *E. coli*, mammalian cells) to produce purified, functional proteins. These recombinant variants retain the ability to bind and remodel actin, enabling researchers to study their biochemical interactions, structural properties, and regulatory mechanisms *in vitro*.
The development of DSTN recombinant proteins has advanced research in cell biology, particularly in understanding actin-driven processes. For example, they are used to dissect signaling pathways involving Rho GTPases, which modulate DSTN activity, or to screen small molecules targeting actin dynamics for therapeutic applications. Additionally, recombinant DSTN serves as a tool in disease models, such as studying tumor cell invasion or neuronal growth cone behavior.
Despite its utility, challenges remain in optimizing recombinant DSTN for specific experimental or clinical needs, including post-translational modifications or isoform-specific functions. Ongoing efforts focus on enhancing stability, activity, and delivery methods, positioning DSTN recombinant proteins as versatile tools for both basic research and drug discovery.
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