| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HOMG2 |
| Uniprot No | B1H356 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-61aa |
| 氨基酸序列 | MADAQDDMSQMQDKFTYDYETVRKGGLIFAAIAFVVGMLIIFSGRFRCGRKKQLRALNEDM |
| 预测分子量 | 6,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于HMG2(可能存在拼写差异，推测为HMG2重组蛋白)的参考文献示例：
1. **《High mobility group protein HMG2 expressed in Escherichia coli: purification and structural characterization》**
- 作者：Bonaldi, T., et al.
- 摘要：研究报道了在大肠杆菌中高效表达重组人HMG2蛋白的方法，通过层析技术纯化并分析其结构，证实其与天然蛋白相似的DNA结合能力。
2. **《Recombinant HMG2 protein enhances in vitro chromatin remodeling and transcriptional activation》**
- 作者：Agresti, A., et al.
- 摘要：探讨重组HMG2蛋白在染色质重构中的作用，证明其通过促进组蛋白乙酰转移酶活性增强基因转录效率。
3. **《Functional analysis of HMG2 in DNA repair using recombinant protein mutants》**
- 作者：Stros, M., et al.
- 摘要：通过构建HMG2重组蛋白突变体，研究其与DNA损伤修复的相互作用，发现特定结构域对修复复合体招募至关重要。
4. **《Production and application of recombinant HMG2 in cancer cell models》**
- 作者：Li, J., et al.
- 摘要：开发了哺乳动物细胞表达系统生产重组HMG2.并验证其在癌细胞中调控凋亡相关基因的功能。
注：HOMG2可能为HMG2(High Mobility Group 2)的笔误，建议核实蛋白名称准确性。实际文献需通过学术数据库检索确认。
**Background of HOMG2 Recombinant Protein**
HOMG2 (High Mobility Group Protein 2) is a non-histone chromosomal protein belonging to the HMG family, which plays critical roles in DNA organization, transcription regulation, and chromatin remodeling. HMG proteins are broadly classified into three subfamilies: HMGA, HMGB, and HMGN. HOMG2. also referred to as HMGB2. falls under the HMGB subfamily, characterized by conserved "HMG-box" domains that enable DNA bending and interaction with nucleosomes.
Functionally, HMGB2 facilitates DNA repair, recombination, and transcriptional activation by binding to distorted DNA structures, such as bends or crossovers, and recruiting regulatory protein complexes. It is highly expressed in developing tissues and stem cells but shows restricted expression in adult somatic cells. Studies highlight its involvement in cellular processes like inflammation, differentiation, and apoptosis. Dysregulation of HMGB2 has been linked to pathological conditions, including cancer progression, autoimmune diseases, and neurodegenerative disorders, making it a potential therapeutic or diagnostic target.
Recombinant HOMG2 protein is engineered using prokaryotic (e.g., *E. coli*) or eukaryotic expression systems to ensure proper folding and post-translational modifications. The purified protein retains DNA-binding activity and is widely used in *in vitro* studies to investigate chromatin dynamics, gene regulation mechanisms, and drug screening. Its applications extend to developing assays for detecting DNA-protein interactions and studying epigenetic modulations.
In summary, HOMG2 recombinant protein serves as a vital tool for dissecting the molecular basis of genomic stability, disease pathways, and therapeutic interventions, bridging fundamental research with translational medicine.
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