| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OSTb |
| Uniprot No | Q86UW2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-128aa |
| 氨基酸序列 | MEHSEGAPGDPAGTVVPQELLEEMLWFFRVEDASPWNHSILALAAVVVIISMVLLGRSIQASRKEKMQPPEKETPEVLHLDEAKDHNSLNNLRETLLSEKPNLAQVELELKERDVLSVFLPDVPETES |
| 预测分子量 | 14,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于OSTb重组蛋白的3篇参考文献示例，涵盖其表达、功能及结构研究：
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1. **文献名称**：*Molecular cloning and functional characterization of the human organic solute transporter beta (OSTβ)*
**作者**：Ballatori, N., et al.
**摘要**：该研究首次克隆并鉴定了人源OSTβ基因，利用哺乳动物细胞表达系统重组表达OSTβ蛋白，证实其与OSTα形成异二聚体后介导胆汁酸跨膜转运，揭示了其在肝脏和肠道中的生理作用。
2. **文献名称**：*Expression and purification of recombinant OSTβ in E. coli for structural studies*
**作者**：Sun, A.Q., et al.
**摘要**：通过优化大肠杆菌表达系统，成功表达并纯化可溶性OSTβ重组蛋白，结合体外功能实验验证其与OSTα的相互作用，为后续结构解析奠定基础。
3. **文献名称**：*Structural insights into the human OSTα-OSTβ transporter complex*
**作者**：Dawson, P.A., et al.
**摘要**：采用昆虫细胞表达系统共表达OSTα和OSTβ重组蛋白，通过冷冻电镜解析复合体结构，阐明两者协同转运有机溶质的分子机制及疾病相关突变的影响。
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注：以上文献信息为示例性质，实际引用时需核对具体文献来源及准确性。
**Background of OSTβ Recombinant Protein**
The OSTβ (Organic Solute Transporter Beta) recombinant protein is a key component of the heterodimeric OSTα-OSTβ transporter, which mediates the cellular efflux of bile acids, sterols, and other organic solutes across plasma membranes. This transporter is critical for maintaining bile acid homeostasis, particularly in the enterohepatic circulation, where it facilitates the reabsorption of bile acids from the intestine to the liver. OSTβ, a type II transmembrane protein, stabilizes and chaperones OSTα to the plasma membrane, enabling their functional coupling.
Recombinant OSTβ is typically produced using expression systems like *E. coli*, mammalian cells, or insect cells, allowing researchers to study its structure, interaction with OSTα, and transport mechanisms in vitro. Its production enables detailed investigations into bile acid dysregulation linked to cholestasis, metabolic disorders, or drug-induced liver toxicity. Additionally, OSTβ recombinant protein aids in screening therapeutic agents targeting bile acid transporters or understanding drug-drug interactions affecting bile acid flux.
Studies using OSTβ knockout models highlight its physiological significance, as deficiencies result in disrupted bile acid recycling and altered lipid metabolism. The recombinant protein also serves as a tool for structural biology, such as crystallography or cryo-EM, to resolve the OSTα-OSTβ complex's architecture. Overall, OSTβ recombinant protein is vital for advancing research on hepatobiliary diseases, metabolic regulation, and drug development tied to organic solute transport pathways.
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