| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TUBa1C |
| Uniprot No | Q9BQE3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-449aa |
| 氨基酸序列 | MRECISIHVGQAGVQIGNACWELYCLEHGIQPDGQMPSDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVIDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIIDLVLDRIRKLADQCTGLQGFLVFHSFGGGTGSGFTSLLMERLSVDYGKKSKLEFSIYPAPQVSTAVVEPYNSILTTHTTLEHSDCAFMVDNEAIYDICRRNLDIERPTYTNLNRLISQIVSSITASLRFDGALNVDLTEFQTNLVPYPRIHFPLATYAPVISAEKAYHEQLTVAEITNACFEPANQMVKCDPRHGKYMACCLLYRGDVVPKDVNAAIATIKTKRTIQFVDWCPTGFKVGINYQPPTVVPGGDLAKVQRAVCMLSNTTAVAEAWARLDHKFDLMYAKRAFVHWYVGEGMEEGEFSEAREDMAALEKDYEEVGADSADGEDEGEEY |
| 预测分子量 | 49,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TUBA1C(α-tubulin 1C)重组蛋白的示例参考文献(内容为虚构示例，建议通过PubMed或SciHub查询真实文献)：
1. **文献名称**: "TUBA1C promotes hepatocellular carcinoma progression by regulating cell cycle"
**作者**: Li X, et al.
**摘要**: 研究证实TUBA1C在肝癌组织中高表达，通过调控Cyclin D1/p21通路加速细胞周期进程，促进肿瘤增殖和转移。
2. **文献名称**: "Recombinant TUBA1C protein enhances microtubule stability in vitro"
**作者**: Smith J, et al.
**摘要**: 通过大肠杆菌表达系统成功纯化TUBA1C重组蛋白，实验显示其显著增强微管聚合稳定性，为体外微管动力学研究提供工具。
3. **文献名称**: "TUBA1C overexpression correlates with poor prognosis in gastric cancer"
**作者**: Wang Y, et al.
**摘要**: 临床数据分析发现TUBA1C高表达与胃癌患者总生存期缩短相关，机制涉及EMT转化和紫杉醇耐药性增强。
4. **文献名称**: "CRISPR/Cas9-mediated TUBA1C knockout disrupts neuronal migration in mice"
**作者**: Chen R, et al.
**摘要**: 利用重组TUBA1C蛋白回补实验，验证了TUBA1C缺失导致神经元微管结构异常，进而影响大脑皮层发育。
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**提示**：实际文献建议通过关键词"TUBA1C recombinant protein"或"TUBA1C overexpression"在学术数据库中检索，重点关注其在癌症、神经发育或药物耐药性领域的研究。
**Background of TUBa1C Recombinant Protein**
TUBa1C, a subtype of α-tubulin, is a critical component of microtubules—dynamic cytoskeletal structures essential for eukaryotic cell functions such as division, intracellular transport, and maintenance of cell shape. As part of the tubulin superfamily, α-tubulin pairs with β-tubulin to form heterodimers, which polymerize into microtubules. The human genome encodes multiple α- and β-tubulin isotypes, each exhibiting tissue-specific expression and functional specialization. TUBa1C (gene: *TUBA1C*) is notably expressed in proliferating cells and certain cancers, where its regulation is linked to cell cycle progression and malignancy.
Recombinant TUBa1C protein is produced using biotechnological platforms, such as *E. coli* or mammalian expression systems, to enable precise study of its biochemical properties. The protein is purified via affinity chromatography, often tagged with markers like His or GST for detection. Its recombinant form retains native folding and polymerization capacity, making it valuable for *in vitro* studies of microtubule dynamics, drug interactions, and structural analyses.
Research on TUBa1C has implications in oncology, as microtubule-targeting agents (e.g., taxanes, vinca alkaloids) are widely used in chemotherapy. Altered expression of specific tubulin isotypes, including TUBa1C, is associated with drug resistance in cancers. Additionally, recombinant TUBa1C aids in exploring neurodegenerative diseases linked to microtubule dysfunction, such as Alzheimer’s, where tubulin dynamics are disrupted.
In summary, TUBa1C recombinant protein serves as a vital tool for dissecting microtubule-related mechanisms in health and disease, bridging structural biology, drug development, and cellular pathophysiology studies.
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