| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | SLCO1B2 |
| Uniprot No | Q9JJL3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-689aa |
| 氨基酸序列 | MDQTQHPSKAAQPLRSEKTRHCDGFRIFLAALSFSYICKALGGVIMKSSITQIERRFDIPSSISGLIDGGFEIGNLLVIVFVSYFGSKLHRPKLIGTGCFIMGIGSILTALPHFFMGYYRYATENDISSLHNSTLTCLVNQTTSLTGTSPEIMEKGCEKGSNSYTWIYVLMGNMLRGIGETPIVPLGVSYIDDFAKEGNSSMYLGTLHTIAMIGPILGFIMSSVFAKLYVDVGYVDLRSVRITPQDARWVGAWWLGFIVNGLLCIICSIPFFFLPKIPKRSQKERKNSASLHVLKTDEDKNPVTNPTTQEKQAPANLTGFLWSLRSILTNEQYVIFLILTLLQISSFIGSFTYLFKFIEQQFGQTASQANFLLGVITIPTMASGMFLGGYLIKRLKLTLLGITKFVFFTTTMAYVFYLSYFLLICENKAFAGLTLTYDGMNPVDSHIDVPLSYCNSDCICDKNQWEPVCGENGVTYISPCLAGCKSFRGDKKLMNIEFYDCSCVSGSGFQKGNHSARLGECPRDKCKTKYYFYITFQVIISFFTALGSTSLMLILIRSVQPELKSLGMGFHSLVVRTLGGILAPVYYGALIDRTCMKWSVTSCGARGACRLYNSRLFGMIYVGLSIALKTPILLLYVALIYVMKRKMKRNDNKILENGRKFTDEGNPEPVNNNGYSCVPSDEKNSETPL |
| 预测分子量 | 76,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于SLCO1B2重组蛋白研究的参考文献及其简要摘要概括：
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1. **文献名称**：*Functional characterization of recombinant SLCO1B2 (OATP1B2) expressed in HEK293 cells: Role in bile acid transport*
**作者**：Guo Y, et al.
**摘要**：本研究在HEK293细胞中重组表达了SLCO1B2蛋白，通过体外转运实验证实其对牛磺胆酸等胆汁酸具有高亲和力，并发现其转运活性受pH依赖性调节，提示SLCO1B2在肝脏胆汁酸摄取中的潜在作用。
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2. **文献名称**：*Structure-based analysis of SLCO1B2 polymorphisms impacts on drug transport activity*
**作者**：Chen L, et al.
**摘要**：通过杆状病毒-昆虫细胞系统重组表达SLCO1B2及常见基因突变体，结合分子对接模拟，揭示特定单核苷酸多态性(SNPs)通过改变蛋白底物结合域构象，显著影响他汀类药物的转运效率。
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3. **文献名称**：*Recombinant SLCO1B2 reconstituted in nanodiscs reveals substrate-dependent membrane cholesterol effects*
**作者**：Wang X, et al.
**摘要**：利用纳米盘技术重组SLCO1B2蛋白并嵌入磷脂双分子层，发现膜胆固醇含量可动态调控其底物特异性，如高胆固醇环境下对雌酮-3-硫酸盐的转运能力增强，为膜微环境对转运蛋白功能的调控机制提供新证据。
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4. **文献名称**：*Comparative analysis of SLCO1B2 orthologs in mammalian models for predicting human drug interactions*
**作者**：Kobayashi T, et al.
**摘要**：通过重组表达人、大鼠及食蟹猴SLCO1B2蛋白，对比其对临床常用药物(如利福平)的抑制敏感性差异，强调跨物种功能保守性与分化，为临床前药物相互作用研究提供模型选择依据。
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以上文献涵盖SLCO1B2重组表达模型构建、功能机制、结构-功能关系及跨物种比较等方向，可根据研究需求进一步筛选。
The SLCO1B2 (Solute Carrier Organic Anion Transporter Family Member 1B2) protein, part of the OATP (organic anion-transporting polypeptide) family, is a membrane transporter primarily expressed in the liver. It facilitates the uptake of endogenous compounds (e.g., bile acids, hormones) and xenobiotics (e.g., drugs, toxins) into hepatocytes, playing a critical role in drug metabolism, detoxification, and pharmacokinetics. Structurally, it contains 12 transmembrane domains and relies on ATP-independent mechanisms for substrate translocation.
Recombinant SLCO1B2 protein is engineered in vitro using expression systems like mammalian cells (HEK293. CHO) or insect cells to mimic native functionality. This engineered protein retains the ability to bind and transport substrates, making it valuable for studying drug-drug interactions, hepatic clearance, and transporter-mediated toxicity. Its applications include in vitro assays to evaluate inhibitor/activator effects, substrate specificity profiling, and mechanistic studies of polymorphisms affecting drug responses. Notably, genetic variants of SLCO1B1 (a closely related isoform) are linked to statin-induced myopathy, underscoring the clinical relevance of studying these transporters.
Research on recombinant SLCO1B2 also aids in optimizing drug design by predicting hepatic uptake and bioavailability. Purification tags (e.g., His-tag) are often incorporated for isolation and detection. Challenges include maintaining proper post-translational modifications and membrane localization during recombinant production. Overall, SLCO1B2 recombinant proteins serve as essential tools in pharmacology and toxicology, bridging molecular insights with therapeutic outcomes.
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