| 纯度 | >90%SDS-PAGE. |
| 种属 | mouse |
| 靶点 | CKAP4 |
| Uniprot No | Q8BMK4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 109-575aa |
| 氨基酸序列 | VLEEVQQVRRGHQDFSRQRDELGQGLQGVEQKVQSLQATFGTFESLLRNS QHKQDLTEKAVKEGESELNRISEVLQKLQNEILKDLSDGIHVVKDARERD FTSLENTVEERLTELTKSINDNIAIFTDVQKRSQKEINEVKMKVASLEES KGDRSQDVKTLKDAVKEVQASMMSRERDIEALKSSLQTMESDVYTEVREL VSLKQEQQAFKQAADSERLALQALTEKLLRSEESSSRLPEDIRRLEEELQ QLKVGAHGSEEGAVFKDSKALEELQRQIEGLGARLQYVEDGVYSMQVASA RHTESLESLLSKSQEYEQRLAMLQEHVGNLGSSSDLASTVRSLGETQLAL SSDLKELKQSLGELPGTVESLQEQVLSLLSQDQAQAEGLPPQDFLDRLSS LDNLKSSVSQVESDLKMLRTAVDSLVAYSVKIETNENNLESAKGLLDDLR NDLDRLFLKVEKIHEKI |
| 预测分子量 | 57 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CKAP4重组蛋白的3篇参考文献示例(文献信息为虚构，供参考)：
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1. **文献名称**：*CKAP4 as a novel DKK1 receptor promotes tumor metastasis via Wnt/β-catenin pathway*
**作者**：Li X, et al.
**摘要**：该研究揭示了CKAP4重组蛋白在肿瘤转移中的作用，证实其作为DKK1(Dickkopf-1)的功能性受体，通过激活Wnt/β-catenin信号通路促进癌细胞侵袭和迁移。实验通过重组CKAP4蛋白验证了其与DKK1的结合能力及下游分子机制。
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2. **文献名称**：*Recombinant CKAP4 protein inhibits pulmonary fibrosis by targeting TGF-β1 signaling*
**作者**：Wang Y, et al.
**摘要**：研究利用重组CKAP4蛋白探究其在肺纤维化中的治疗潜力。结果表明，CKAP4通过阻断TGF-β1/Smad3信号通路抑制成纤维细胞活化，减轻小鼠模型中的纤维化病变，提示其作为抗纤维化治疗靶点的可能性。
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3. **文献名称**：*Structural and functional characterization of CKAP4 recombinant protein in endoplasmic reticulum homeostasis*
**作者**：Saito K, et al.
**摘要**：该研究通过表达纯化CKAP4重组蛋白，解析其与内质网分子伴侣GRP78的相互作用结构，证实CKAP4参与内质网应激反应的调控，为相关疾病(如癌症、神经退行性疾病)的机制研究提供新视角。
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**注**：以上文献为示例性内容，实际研究中请通过PubMed、Web of Science等平台检索真实文献。
**Background on CKAP4 Recombinant Protein**
Cytoskeleton-associated protein 4 (CKAP4), also known as cytoskeleton-linking membrane protein 4 (CLIMP-63) or ERGIC-53-like protein, is a transmembrane protein primarily localized in the endoplasmic reticulum (ER) and plasma membrane. It plays a multifaceted role in cellular processes, including ER structural organization, vesicle trafficking, and cytoskeleton dynamics. Structurally, CKAP4 contains an N-terminal coiled-coil domain, a transmembrane domain, and a C-terminal cytoplasmic tail, enabling interactions with microtubules and other cytoskeletal components.
CKAP4 has garnered attention for its dual role as a receptor and signaling mediator. It binds to diverse ligands, such as surfactant protein A (SP-A) in lung immune responses and anti-CKAP4 autoantibodies in certain cancers. In oncology, CKAP4 is implicated in tumor progression, metastasis, and drug resistance. Overexpression of CKAP4 correlates with poor prognosis in cancers like pancreatic, gastric, and lung adenocarcinoma, making it a potential diagnostic biomarker and therapeutic target.
Recombinant CKAP4 protein is produced via heterologous expression systems, such as *E. coli* or mammalian cell lines, followed by purification using affinity chromatography. This engineered protein retains functional domains, enabling studies on ligand-receptor interactions, intracellular signaling pathways, and its role in cancer biology. Researchers utilize CKAP4 recombinant protein to develop targeted therapies, including monoclonal antibodies and small-molecule inhibitors, as well as diagnostic tools for detecting CKAP4 in patient samples.
Beyond cancer, CKAP4 recombinant protein aids in exploring its physiological functions, such as ER-mitochondria communication and cellular stress responses. Its versatility in both basic research and clinical applications underscores its significance in understanding disease mechanisms and advancing precision medicine.
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