| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FOXF2 |
| Uniprot No | Q12947 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-444aa |
| 氨基酸序列 | MTTEGGPPPA PLRRACSPVP GALQAALMSP PPAAAAAAAA APETTSSSSS SSSASCASSS SSSNSASAPS AACKSAGGGG AGAGSGGAKK ASSGLRRPEK PPYSYIALIV MAIQSSPSKR LTLSEIYQFL QARFPFFRGA YQGWKNSVRH NLSLNECFIK LPKGLGRPGK GHYWTIDPAS EFMFEEGSFR RRPRGFRRKC QALKPMYHRV VSGLGFGASL LPQGFDFQAP PSAPLGCHSQ GGYGGLDMMP AGYDAGAGAP SHAHPHHHHH HHVPHMSPNP GSTYMASCPV PAGPGGVGAA GGGGGGDYGP DSSSSPVPSS PAMASAIECH SPYTSPAAHW SSPGASPYLK QPPALTPSSN PAASAGLHSS MSSYSLEQSY LHQNAREDLS VGLPRYQHHS TPVCDRKDFV LNFNGISSFH PSASGSYYHH HHQSVCQDIK PCVM |
| 预测分子量 | 45,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FOXF2重组蛋白的3篇参考文献及其摘要概括：
1. **《FOXF2 suppresses the FOXC2-mediated epithelial-mesenchymal transition in breast cancer》**
- **作者**: Zhang, J. et al.
- **摘要**: 研究揭示FOXF2重组蛋白通过抑制FOXC2信号通路，阻碍乳腺癌细胞的上皮-间质转化(EMT)，从而抑制肿瘤转移。
2. **《FOXF2 regulates mesenchymal differentiation and metastasis in colorectal cancer》**
- **作者**: Xu, Y. et al.
- **摘要**: 发现FOXF2重组蛋白在结肠癌中通过调控Wnt/β-catenin通路，抑制间充质表型转化，降低肿瘤侵袭性。
3. **《Recombinant FOXF2 protein attenuates pulmonary fibrosis via antagonizing TGF-β1 signaling》**
- **作者**: Chen, L. et al.
- **摘要**: 研究表明，体外表达的FOXF2重组蛋白可抑制TGF-β1介导的成纤维细胞活化，减少胶原沉积，缓解肺纤维化进程。
4. **《Structural and functional analysis of FOXF2 recombinant protein in neural crest development》**
- **作者**: Kim, S. et al.
- **摘要**: 通过重组FOXF2蛋白的功能实验，证实其在小鼠胚胎神经嵴细胞分化中起关键作用，影响颅面部发育。
(注：以上文献为示例性内容，实际引用需根据具体研究补充完整信息。)
FOXF2 recombinant protein is a engineered version of the Forkhead Box F2 (FOXF2) transcription factor, designed for biomedical research applications. As a member of the Forkhead family, FOXF2 contains a conserved winged-helix DNA-binding domain that enables sequence-specific interactions with gene promoters. This protein plays critical roles in embryonic development, particularly in mesenchymal tissue formation, organogenesis, and cellular differentiation.
Originally identified through homology with the Drosophila forkhead gene, FOXF2 is predominantly expressed in mesenchymal cells during embryogenesis. It regulates tissue patterning through interactions with signaling pathways like TGF-β and BMP. In adults, FOXF2 maintains tissue homeostasis and exhibits tumor-suppressive properties by inhibiting epithelial-mesenchymal transition (EMT) in cancers.
The recombinant form is typically produced in E. coli or mammalian expression systems, featuring tags like His or GST for purification and detection. Researchers employ it to study developmental biology mechanisms, wound healing processes, and cancer progression models. Its functional domains (N-terminal activation domain and C-terminal inhibitory domain) make it particularly valuable for investigating transcriptional regulation dynamics.
Current research focuses on FOXF2's dual role in tumorigenesis – promoting differentiation while suppressing metastasis – and its involvement in genetic disorders like craniofacial abnormalities. The recombinant protein serves as essential material for binding assays, cellular localization studies, and therapeutic target validation, bridging structural biology with clinical applications in regenerative medicine and oncology.
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