| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GNaS1 |
| Uniprot No | P63092 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-394aa |
| 氨基酸序列 | MGCLGNSKTEDQRNEEKAQREANKKIEKQLQKDKQVYRATHRLLLLGAGESGKSTIVKQMRILHVNGFNGEGGEEDPQAARSNSDGEKATKVQDIKNNLKEAIETIVAAMSNLVPPVELANPENQFRVDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDVIKQADYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHMFDVGGQRDERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL |
| 预测分子量 | 61.7kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GNAS1(G蛋白α亚基)重组蛋白的3篇模拟参考文献示例(注：部分内容为简化概括，实际文献需通过数据库检索确认)：
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1. **文献名称**: *"Expression and Purification of Recombinant GNAS1 in E. coli for Functional Studies"*
**作者**: Smith J, et al.
**摘要**: 本研究报道了人源GNAS1基因在大肠杆菌中的重组表达及纯化方法，通过优化诱导条件获得高纯度蛋白，并验证了其GTPase活性，为后续信号通路研究提供工具。
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2. **文献名称**: *"Structural Insights into GNAS1 Mutations in Hormone Resistance Syndromes"*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析GNAS1重组蛋白的突变体结构，揭示了特定突变(如R201H)如何干扰G蛋白偶联受体信号传导，导致假性甲状旁腺功能减退症等疾病。
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3. **文献名称**: *"In Vitro Reconstitution of GNAS1-Dependent cAMP Signaling Pathway"*
**作者**: Garcia-Reyes M, et al.
**摘要**: 利用重组GNAS1蛋白在体外重建cAMP信号通路，证明其与腺苷酸环化酶的相互作用机制，并评估了不同突变对第二信使产生的动态影响。
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如需具体领域文献(如疾病机制/药物筛选)，建议补充关键词后检索PubMed或SciFinder数据库获取真实文献。
**Background of GNaS1 Recombinant Protein**
The GNaS1 recombinant protein is a genetically engineered protein derived from the human GNAS1 gene, which encodes the stimulatory alpha subunit of heterotrimeric G-proteins (Gαs). This protein plays a pivotal role in intracellular signaling by activating adenylate cyclase upon receptor-mediated stimulation, leading to the production of cyclic AMP (cAMP), a critical secondary messenger regulating diverse physiological processes, including metabolism, cell growth, and hormone secretion.
Recombinant GNaS1 is typically produced using expression systems such as *E. coli* or mammalian cell cultures, ensuring high purity and bioactivity for research and therapeutic applications. Its production involves cloning the GNAS1 gene into expression vectors, followed by purification via affinity chromatography (e.g., His-tag or GST-tag systems).
Mutations in the GNAS1 gene are linked to several diseases, such as McCune-Albright syndrome, pseudohypoparathyroidism, and endocrine tumors, making GNaS1 a focus for studying G-protein-coupled receptor (GPCR) signaling dysregulation. The recombinant form enables mechanistic studies, drug screening, and structural analyses to elucidate interactions with GPCRs, effectors, and regulatory proteins.
In drug development, GNaS1 serves as a tool to validate targets for disorders involving cAMP pathway imbalances. It also aids in diagnosing genetic mutations via *in vitro* assays. Despite its utility, challenges remain in mimicking post-translational modifications critical for its function, necessitating advanced expression systems.
Overall, GNaS1 recombinant protein bridges basic research and clinical applications, offering insights into cellular signaling and therapeutic innovation.
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