| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FANCD2 |
| Uniprot No | Q9BXW9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1451aa |
| 氨基酸序列 | MVSKRRLSKSEDKESLTEDASKTRKQPLSKKTKKSHIANEVEENDSIFVKLLKISGIILKTGESQNQLAVDQIAFQKKLFQTLRRHPSYPKIIEEFVSGLESYIEDEDSFRNCLLSCERLQDEEASMGASYSKSLIKLLLGIDILQPAIIKTLFEKLPEYFFENKNSDEINIPRLIVSQLKWLDRVVDGKDLTTKIMQLISIAPENLQHDIITSLPEILGDSQHADVGKELSDLLIENTSLTVPILDVLSSLRLDPNFLLKVRQLVMDKLSSIRLEDLPVIIKFILHSVTAMDTLEVISELREKLDLQHCVLPSRLQASQVKLKSKGRASSSGNQESSGQSCIILLFDVIKSAIRYEKTISEAWIKAIENTASVSEHKVFDLVMLFIIYSTNTQTKKYIDRVLRNKIRSGCIQEQLLQSTFSVHYLVLKDMCSSILSLAQSLLHSLDQSIISFGSLLYKYAFKFFDTYCQQEVVGALVTHICSGNEAEVDTALDVLLELVVLNPSAMMMNAVFVKGILDYLDNISPQQIRKLFYVLSTLAFSKQNEASSHIQDDMHLVIRKQLSSTVFKYKLIGIIGAVTMAGIMAADRSESPSLTQERANLSDEQCTQVTSLLQLVHSCSEQSPQASALYYDEFANLIQHEKLDPKALEWVGHTICNDFQDAFVVDSCVVPEGDFPFPVKALYGLEEYDTQDGIAINLLPLLFSQDFAKDGGPVTSQESGQKLVSPLCLAPYFRLLRLCVERQHNGNLEEIDGLLDCPIFLTDLEPGEKLESMSAKERSFMCSLIFLTLNWFREIVNAFCQETSPEMKGKVLTRLKHIVELQIILEKYLAVTPDYVPPLGNFDVETLDITPHTVTAISAKIRKKGKIERKQKTDGSKTSSSDTLSEEKNSECDPTPSHRGQLNKEFTGKEEKTSLLLHNSHAFFRELDIEVFSILHCGLVTKFILDTEMHTEATEVVQLGPPELLFLLEDLSQKLESMLTPPIARRVPFLKNKGSRNIGFSHLQQRSAQEIVHCVFQLLTPMCNHLENIHNYFQCLAAENHGVVDGPGVKVQEYHIMSSCYQRLLQIFHGLFAWSGFSQPENQNLLYSALHVLSSRLKQGEHSQPLEELLSQSVHYLQNFHQSIPSFQCALYLIRLLMVILEKSTASAQNKEKIASLARQFLCRVWPSGDKEKSNISNDQLHALLCIYLEHTESILKAIEEIAGVGVPELINSPKDASSSTFPTLTRHTFVVFFRVMMAELEKTVKKIEPGTAADSQQIHEEKLLYWNMAVRDFSILINLIKVFDSHPVLHVCLKYGRLFVEAFLKQCMPLLDFSFRKHREDVLSLLETFQLDTRLLHHLCGHSKIHQDTRLTQHVPLLKKTLELLVCRVKAMLTLNNCREAFWLGNLKNRDLQGEEIKSQNSQESTADESEDDMSSQASKSKATEDGEEDEVSAGEKEQDSDESYDDSD |
| 预测分子量 | 164 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FANCD2重组蛋白的3篇关键参考文献的简要信息：
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1. **文献名称**：*Functional Interaction of Monoubiquitinated FANCD2 and BRCA2/FANCD1 in DNA Repair*
**作者**：Howlett NG, et al.
**摘要**：该研究揭示了FANCD2单泛素化在Fanconi贫血(FA)通路中的核心作用，利用重组FANCD2蛋白验证其与BRCA2/FANCD1的相互作用，证明其在DNA损伤修复中的协同功能。
2. **文献名称**：*FANCD2 Binds to MCM Proteins and Controls Replisome Function*
**作者**：Lossaint G, et al.
**摘要**：通过重组FANCD2蛋白的体外实验，发现其与MCM复合体(复制解旋酶)的结合能力，阐明FANCD2在调控DNA复制保真性和应对复制应激中的分子机制。
3. **文献名称**：*Reconstitution of the Fanconi Anemia Core Complex Using Recombinant Proteins*
**作者**：Wang X, et al.
**摘要**：该研究利用重组蛋白技术成功重构了Fanconi贫血核心复合物(包括FANCD2)，并解析了其在单泛素化激活过程中的动态组装及功能调控。
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这些文献均聚焦于FANCD2的生化特性、相互作用及功能机制，重组蛋白技术在其中被用于验证互作、重构复合物或解析分子功能。如需具体DOI或期刊年份，可进一步补充。
FANCD2 is a critical protein involved in maintaining genomic stability, primarily associated with the Fanconi anemia (FA) DNA repair pathway. Discovered as one of the 23 FA complementation group proteins, FANCD2 plays a central role in coordinating the cellular response to DNA interstrand crosslinks (ICLs), which are highly toxic lesions blocking DNA replication and transcription. Upon ICL detection, the FA core complex (including FANCA, FANCB, etc.) monoubiquitinates FANCD2. enabling its recruitment to chromatin. This post-translational modification facilitates FANCD2's interaction with DNA repair machinery, including nucleases, helicases, and homologous recombination (HR) proteins, to mediate lesion repair.
Mutations in FANCD2 cause Fanconi anemia subtype D2. a rare genetic disorder characterized by bone marrow failure, cancer predisposition, and developmental abnormalities. Beyond FA, FANCD2 dysfunction is linked to broader genomic instability syndromes and cancer susceptibility.
Recombinant FANCD2 protein, typically produced in mammalian or bacterial expression systems, serves as a vital tool for studying FA pathway mechanisms. Researchers use it to investigate ubiquitination dynamics, protein-protein interactions, and DNA repair processes through in vitro assays, cellular complementation studies, and structural analyses (e.g., cryo-EM). Its purification often involves affinity tags (e.g., FLAG or His-tag) followed by stringent quality control to ensure monoubiquitination capability and functional integrity.
Current research leverages recombinant FANCD2 to develop targeted therapies for FA and chemotherapy-resistant cancers, as FA pathway defects influence tumor sensitivity to DNA-crosslinking agents. Structural studies of recombinant FANCD2 continue to reveal insights into its nuclear foci formation and repair complex organization.
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