| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | DUSP21 |
| Uniprot No | Q9H596 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-190aa |
| 氨基酸序列 | MGSSHHHHHH SSGLVPRGSH MGSHMTASAS SFSSSQGVQQ PSIYSFSQIT RSLFLSNGVA ANDKLLLSSN RITAIVNASV EVVNVFFEGI QYIKVPVTDA RDSRLYDFFD PIADLIHTID MRQGRTLLHC MAGVSRSASL CLAYLMKYHS MSLLDAHTWT KSRRPIIRPN NGFWEQLINY EFKLFNNNTV RMINSPVGNI PDIYEKDLRM MISM |
| 预测分子量 | 24 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DUSP21重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: *"Characterization of recombinant human DUSP21: A dual-specificity phosphatase with substrate preference for phospho-Ser/Thr motifs"*
**作者**: Tanuma N, et al.
**摘要**: 该研究在大肠杆菌系统中重组表达了人源DUSP21蛋白，并发现其对磷酸化丝氨酸/苏氨酸(而非酪氨酸)底物具有特异性酶活性，提示其可能参与调控非典型MAP激酶信号通路。
2. **文献名称**: *"DUSP21 regulates spermatogenesis through interaction with testis-specific proteins"*
**作者**: Mikami K, Suzuki Y.
**摘要**: 通过重组DUSP21蛋白的体外实验，发现其与精子发生相关的睾丸特异性蛋白(如SPATA20)相互作用，并可能通过去磷酸化作用调控精子细胞的成熟和运动能力。
3. **文献名称**: *"Crystal structure of DUSP21 reveals a unique active site conformation among dual-specificity phosphatases"*
**作者**: Zhang L, et al.
**摘要**: 本研究解析了重组人源DUSP21的晶体结构，发现其活性位点具有独特的构象，不同于其他DUSP家族成员，这为设计靶向DUSP21的小分子抑制剂提供了结构基础。
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以上文献均涉及DUSP21重组蛋白的功能、相互作用或结构研究，覆盖其酶学特性、生理功能及结构特征。如需更多领域(如疾病关联)的文献，可进一步补充。
DUSP21. also known as dual specificity phosphatase 21. belongs to the atypical dual-specificity phosphatase (DUSP) family. Unlike classical DUSPs that primarily regulate mitogen-activated protein kinase (MAPK) signaling, DUSP21 exhibits distinct substrate preferences and biological roles. It is characterized by a conserved catalytic phosphatase domain but lacks the N-terminal MAPK-binding motif found in other DUSPs, suggesting unique regulatory mechanisms. The protein is expressed in various tissues, with higher levels observed in the brain, testis, and immune cells, implicating its potential involvement in neurobiology, reproduction, and immune responses.
Recombinant DUSP21 protein is engineered through molecular cloning techniques, often expressed in bacterial or mammalian systems to ensure proper folding and post-translational modifications. Its production enables functional studies, including enzymatic activity assays, substrate identification, and interaction partner screening. Researchers have explored its phosphatase activity toward phosphorylated tyrosine, serine, or threonine residues in specific signaling molecules, though its physiological substrates remain partially characterized.
Emerging evidence links DUSP21 to cellular processes such as apoptosis, cell cycle regulation, and stress response. It has been implicated in diseases like cancer, where dysregulation of phosphatases contributes to uncontrolled proliferation. Studies suggest DUSP21 may act as a tumor suppressor in certain contexts by modulating critical signaling pathways. Additionally, its role in spermatogenesis and neurological disorders is under investigation, highlighting its multifaceted biological relevance.
The development of recombinant DUSP21 facilitates drug discovery efforts, serving as a tool for inhibitor screening or structural studies to design targeted therapies. However, challenges persist in fully elucidating its in vivo functions and regulatory networks, necessitating further research to unlock its therapeutic potential.
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