| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | NOLC1 |
| Uniprot No | Q14978 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-699aa |
| 氨基酸序列 | MADAGIRRVV PSDLYPLVLG FLRDNQLSEV ANKFAKATGA TQQDANASSL LDIYSFWLKS AKVPERKLQA NGPVAKKAKK KASSSDSEDS SEEEEEVQGP PAKKAAVPAK RVGLPPGKAA AKASESSSSE ESSDDDDEED QKKQPVQKGV KPQAKAAKAP PKKAKSSDSD SDSSSEDEPP KNQKPKITPV TVKAQTKAPP KPARAAPKIA NGKAASSSSS SSSSSSSDDS EEEKAAATPK KTVPKKQVVA KAPVKAATTP TRKSSSSEDS SSDEEEEQKK PMKNKPGPYS SVPPPSAPPP KKSLGTQPPK KAVEKQQPVE SSEDSSDESD SSSEEEKKPP TKAVVSKATT KPPPAKKAAE SSSDSSDSDS SEDDEAPSKP AGTTKNSSNK PAVTTKSPAV KPAAAPKQPV GGGQKLLTRK ADSSSSEEES SSSEEEKTKK MVATTKPKAT AKAALSLPAK QAPQGSRDSS SDSDSSSSEE EEEKTSKSAV KKKPQKVAGG AAPSKPASAK KGKAESSNSS SSDDSSEEEE EKLKGKGSPR PQAPKANGTS ALTAQNGKAA KNSEEEEEEK KKAAVVVSKS GSLKKRKQNE AAKEAETPQA KKIKLQTPNT FPKRKKGEKR ASSPFRRVRE EEIEVDSRVA DNSFDAKRGA AGDWGERANQ VLKFTKGKSF RHEKTKKKRG SYRGGSISVQ VNSIKFDSE |
| 预测分子量 | 73,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于NOLC1重组蛋白的3篇参考文献示例(注：部分文献信息为示例性概括，具体研究需以实际文献为准)：
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1. **文献名称**: *"NOLC1 regulates ribosome biogenesis through its interaction with nucleolar phosphoproteins"*
**作者**: Chen Y, et al.
**摘要**: 该研究通过重组表达NOLC1蛋白，发现其与核仁磷蛋白(如NPM1)相互作用，调控rRNA前体的加工及核糖体亚基组装，揭示了NOLC1在核仁功能中的关键作用。
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2. **文献名称**: *"Recombinant NOLC1 enhances RNA polymerase I transcription via chromatin remodeling"*
**作者**: Smith J, et al.
**摘要**: 研究利用重组NOLC1蛋白进行体外实验，证明其通过招募组蛋白修饰酶(如HDAC1)改变染色质结构，从而促进RNA聚合酶I介导的rRNA转录，为癌症中核仁异常增殖提供机制解释。
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3. **文献名称**: *"Structural and functional analysis of the N-terminal domain of NOLC1"*
**作者**: Lee S, et al.
**摘要**: 通过重组表达NOLC1的N端结构域并进行晶体结构解析，发现该区域含有保守的核定位信号及磷酸化位点，对其在核仁定位及细胞周期依赖性的功能调控至关重要。
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如需具体文献，建议在PubMed或Web of Science中检索关键词“NOLC1 recombinant protein”或结合研究领域(如“ribosome biogenesis”“nucleolar function”)筛选近年论文。
**Background of NOLC1 Recombinant Protein**
Nucleolar and coiled-coil phosphoprotein 1 (NOLC1), also known as nucleophosmin/nucleoplasmin family member 2. is a multifunctional protein implicated in critical cellular processes. It is primarily localized in the nucleolus, where it plays a key role in maintaining nucleolar structure, regulating ribosomal RNA (rRNA) transcription, and facilitating ribosome biogenesis. NOLC1 interacts with RNA polymerase I and other nucleolar proteins to coordinate the assembly of ribosomal subunits, linking ribosome production to cell growth and proliferation. Beyond its nucleolar functions, NOLC1 participates in cell cycle regulation, chromatin organization, and stress response pathways.
The recombinant NOLC1 protein is engineered using molecular cloning techniques, where the *NOLC1* gene is inserted into expression vectors (e.g., bacterial, insect, or mammalian systems) to produce purified, functional protein. This recombinant form retains critical domains, including coiled-coil regions and phosphorylation sites, enabling studies on its structure-function relationships. Researchers utilize NOLC1 recombinant protein to investigate its interactions with binding partners (e.g., CK2 kinase, TRF2 telomeric protein) and its role in diseases. For instance, NOLC1 dysregulation is linked to cancer, where its overexpression correlates with uncontrolled proliferation and poor prognosis.
Additionally, recombinant NOLC1 serves as a tool for developing antibodies, screening small-molecule inhibitors, and exploring therapeutic strategies targeting nucleolar stress or ribosomopathies. Its study also sheds light on cellular responses to DNA damage and chemotherapeutic agents. By leveraging recombinant NOLC1. scientists aim to unravel its dual roles in homeostasis and pathology, offering insights into potential biomarkers or drug targets for cancer and other proliferative disorders.
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