| 纯度 | >90%SDS-PAGE. |
| 种属 | E.coli |
| 靶点 | DREB3 |
| Uniprot No | Q9LYD3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-236aa |
| 氨基酸序列 | MAEEYYSLRS ERVTQLLVPN SESDSVSDKS KAEQSEKKTK RGRDSGKHPV YRGVRMRNWG KWVSEIREPR KKSRIWLGTF PTPEMAARAH DVAALSIKGT AAILNFPELA DSFPRPVSLS PRDIQTAALK AAHMEPTTSF SSSTSSSSSL SSTSSLESLV LVMDLSRTES EELGEIVELP SLGASYDVDS ANLGNEFVFY DSVDYCLYPP PWGQSSEDNY GHGISPNFGH GLSWDL |
| 预测分子量 | 26 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于DREB3重组蛋白的参考文献示例，基于相关领域的研究方向和典型文献结构整理：
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1. **文献名称**: *Functional Analysis of the DREB3 Transcription Factor in Arabidopsis thaliana via Recombinant Protein Expression*
**作者**: Sakuma, Y., Liu, Q., Dubouzet, J.G., Yamaguchi-Shinozaki, K.
**摘要**: 本研究通过在大肠杆菌中表达重组DREB3蛋白，验证其与脱水响应元件(DRE)的结合能力。实验表明，DREB3能够特异性识别DRE序列，并在拟南芥中过表达后显著增强植株的耐旱性和耐盐性，表明其作为转录激活因子的关键作用。
2. **文献名称**: *Heterologous Expression and Characterization of Wheat DREB3 in E. coli for Stress Tolerance Studies*
**作者**: Chen, L., Wang, Z., Zhang, H.
**摘要**: 通过在大肠杆菌系统中表达小麦来源的重组DREB3蛋白，研究其理化特性及DNA结合功能。纯化后的蛋白在体外凝胶迁移实验(EMSA)中显示出对DRE序列的高亲和力，为后续植物遗传改良提供了分子基础。
3. **文献名称**: *Structural Insights into DREB3-DNA Interaction via X-ray Crystallography*
**作者**: Nakashima, K., Ito, Y., Shinozaki, K.
**摘要**: 利用重组DREB3蛋白的晶体结构解析，揭示了其AP2结构域与DRE元件的结合机制。研究发现，DREB3通过特定的β-折叠结构与DNA结合，突变关键氨基酸残基会显著削弱其转录激活能力。
4. **文献名称**: *Overexpression of Recombinant DREB3 in Soybean Enhances Osmotic Stress Resistance*
**作者**: Wang, H., Li, J., Wei, Y.
**摘要**: 在大豆中过表达重组DREB3蛋白，显著提高了植株在干旱和高盐条件下的存活率。转录组分析显示，DREB3通过调控下游胁迫响应基因(如RD29A和COR15A)的表达增强抗逆性。
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**说明**：以上文献为示例性质，实际引用时需通过学术数据库(如PubMed、Web of Science)核实具体信息。DREB3相关研究多集中于其在非生物胁迫响应中的调控机制，重组蛋白的应用常见于功能验证或结构解析领域。
**Background of DREB3 Recombinant Protein**
DREB3 (Dehydration-Responsive Element-Binding protein 3) belongs to the AP2/ERF (APETALA2/Ethylene-Responsive Factor) family of transcription factors, which play critical roles in plant responses to abiotic stresses such as drought, salinity, and cold. These proteins specifically bind to dehydration-responsive elements (DRE)/C-repeat (CRT) cis-acting sequences in the promoters of stress-inducible genes (e.g., *rd29A* and *cor15a*), activating their expression to enhance stress tolerance.
DREB3 is categorized within the DREB subfamily, which is further divided into A-1 to A-6 subgroups based on sequence homology. While DREB1/CBF and DREB2 are well-studied for their roles in cold and osmotic stress responses, DREB3 represents a less characterized member, with functional variations observed across plant species. For instance, in *Arabidopsis*, DREB3 (At3g11020) shows distinct expression patterns and regulatory mechanisms compared to DREB1/2. potentially acting through ABA-independent pathways.
Recombinant DREB3 proteins are engineered to study their structure-function relationships or improve plant stress resilience via genetic engineering. Produced in heterologous systems like *E. coli* or yeast, these proteins retain the conserved AP2 DNA-binding domain and nuclear localization signals, enabling targeted gene regulation. Overexpression of DREB3 in transgenic plants has been linked to enhanced stress tolerance, though excessive activation may impair growth, necessitating precise regulation.
Research on DREB3 highlights its potential in developing climate-resilient crops. However, species-specific functional divergence and regulatory crosstalk with other stress pathways (e.g., ABA signaling) require deeper exploration to optimize biotechnological applications.
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