| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | EIF2aK4 |
| Uniprot No | Q9P2K8 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1649aa |
| 氨基酸序列 | MAGGRGAPGRGRDEPPESYPQRQDHELQALEAIYGADFQDLRPDACGPVKEPPEINLVLYPQGLTGEEVYVKVDLRVKCPPTYPDVVPEIELKNAKGLSNESVNLLKSRLEELAKKHCGEVMIFELAYHVQSFLSEHNKPPPKSFHEEMLERRAQEEQQRLLEAKRKEEQEQREILHEIQRRKEEIKEEKKRKEMAKQERLEIASLSNQDHTSKKDPGGHRTAAILHGGSPDFVGNGKHRANSSGRSRRERQYSVCNSEDSPGSCEILYFNMGSPDQLMVHKGKCIGSDEQLGKLVYNALETATGGFVLLYEWVLQWQKKMGPFLTSQEKEKIDKCKKQIQGTETEFNSLVKLSHPNVVRYLAMNLKEQDDSIVVDILVEHISGVSLAAHLSHSGPIPVHQLRRYTAQLLSGLDYLHSNSVVHKVLSASNVLVDAEGTVKITDYSISKRLADICKEDVFEQTRVRFSDNALPYKTGKKGDVWRLGLLLLSLSQGQECGEYPVTIPSDLPADFQDFLKKCVCLDDKERWSPQQLLKHSFINPQPKMPLVEQSPEDSEGQDYVETVIPSNRLPSAAFFSETQRQFSRYFIEFEELQLLGKGAFGAVIKVQNKLDGCCYAVKRIPINPASRQFRRIKGEVTLLSRLHHENIVRYYNAWIERHERPAGPGTPPPDSGPLAKDDRAARGQPASDTDGLDSVEAAAPPPILSSSVEWSTSGERSASARFPATGPGSSDDEDDDEDEHGGVFSQSFLPASDSESDIIFDNEDENSKSQNQDEDCNEKNGCHESEPSVTTEAVHYLYIQMEYCEKSTLRDTIDQGLYRDTVRLWRLFREILDGLAYIHEKGMIHRDLKPVNIFLDSDDHVKIGDFGLATDHLAFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGAVQLCTPLLLPRNRQIYEHNEAALFMDHSGMLVMLPFDLRIPFARYVARNNILNLKRYCIERVFRPRKLDRFHPKELLECAFDIVTSTTNSFLPTAEIIYTIYEIIQEFPALQERNYSIYLNHTMLLKAILLHCGIPEDKLSQVYIILYDAVTEKLTRREVEAKFCNLSLSSNSLCRLYKFIEQKGDLQDLMPTINSLIKQKTGIAQLVKYGLKDLEEVVGLLKKLGIKLQVLINLGLVYKVQQHNGIIFQFVAFIKRRQRAVPEILAAGGRYDLLIPQFRGPQALGPVPTAIGVSIAIDKISAAVLNMEESVTISSCDLLVVSVGQMSMSRAINLTQKLWTAGITAEIMYDWSQSQEELQEYCRHHEITYVALVSDKEGSHVKVKSFEKERQTEKRVLETELVDHVLQKLRTKVTDERNGREASDNLAVQNLKGSFSNASGLFEIHGATVVPIVSVLAPEKLSASTRRRYETQVQTRLQTSLANLHQKSSEIEILAVDLPKETILQFLSLEWDADEQAFNTTVKQLLSRLPKQRYLKLVCDEIYNIKVEKKVSVLFLYSYRDDYYRILF |
| 预测分子量 | 186 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于EIF2AK4重组蛋白的3-4条参考文献示例，涵盖不同研究方向：
1. **文献名称**：*"Expression and Purification of Functional Recombinant EIF2AK4 Kinase in Baculovirus System"*
**作者**：Smith et al.
**摘要**：本研究成功利用杆状病毒表达系统制备了重组EIF2AK4蛋白，并通过体外激酶实验验证其磷酸化eIF2α的能力，为后续功能研究提供了可靠工具。
2. **文献名称**：*"Structural Insights into EIF2AK4 Activation Mechanism by Cryo-EM"*
**作者**：Jones et al.
**摘要**：通过冷冻电镜解析了EIF2AK4重组蛋白的激活态结构，揭示了其底物结合域和ATP结合域的构象变化，阐明了氨基酸饥饿下的自抑制调控机制。
3. **文献名称**：*"EIF2AK4 Mutations Disrupt Recombinant Protein Function in Pulmonary Veno-Occlusive Disease"*
**作者**：Zhang et al.
**摘要**：研究证明EIF2AK4重组蛋白中与遗传性肺静脉闭塞病相关的突变(如R611H)会显著削弱其激酶活性，导致细胞应激通路异常。
4. **文献名称**：*"EIF2AK4-Mediated Integrated Stress Response in Amino Acid Starvation"*
**作者**：Brown et al.
**摘要**：利用重组EIF2AK4蛋白，揭示了其在氨基酸缺乏时通过磷酸化eIF2α调控下游基因表达，维持细胞代谢平衡的分子机制。
这些示例覆盖了蛋白制备、结构解析、疾病关联及功能机制等研究方向，符合EIF2AK4重组蛋白的典型研究框架。
EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4), also known as GCN2 (general control nonderepressible 2), is a serine/threonine kinase belonging to the eIF2α kinase family. It plays a critical role in cellular stress response pathways, particularly under amino acid deprivation. EIF2AK4 monitors uncharged tRNA levels during nutrient scarcity, activating its kinase function to phosphorylate the alpha subunit of eukaryotic initiation factor 2 (eIF2α). This phosphorylation event globally reduces protein synthesis while selectively promoting the translation of stress-responsive transcripts, such as ATF4. to restore metabolic homeostasis.
The recombinant EIF2AK4 protein, produced through genetic engineering in systems like *E. coli* or mammalian cells, retains the kinase's functional domains, including the pseudokinase, RWD, and kinase domains. Researchers utilize this purified protein to study its regulatory mechanisms, substrate interactions, and structural features *in vitro*. Its role in diseases, including cancer, neurodegenerative disorders, and pulmonary arterial hypertension, has driven interest in EIF2AK4 as a therapeutic target. For instance, aberrant EIF2AK4 signaling is implicated in tumor survival under nutrient-poor microenvironments and in ischemia-related neuronal damage.
Studies with recombinant EIF2AK4 have facilitated drug discovery efforts, enabling high-throughput screening for kinase inhibitors. Additionally, it aids in deciphering cross-talk with other stress-response pathways, such as the integrated stress response (ISR). Ongoing research explores its tissue-specific functions and potential as a biomarker for diseases linked to proteostasis imbalance. The development of recombinant tools continues to advance our understanding of cellular adaptation to metabolic challenges and pathological stress.
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