| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | CD3eAP |
| Uniprot No | O15446 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-510aa |
| 氨基酸序列 | MEEPQAGDAA RFSCPPNFTA KPPASESPRF SLEALTGPDT ELWLIQAPAD FAPECFNGRH VPLSGSQIVK GKLAGKRHRY RVLSSCPQAG EATLLAPSTE AGGGLTCASA PQGTLRILEG PQQSLSGSPL QPIPASPPPQ IPPGLRPRFC AFGGNPPVTG PRSALAPNLL TSGKKKKEMQ VTEAPVTQEA VNGHGALEVD MALGSPEMDV RKKKKKKNQQ LKEPEAAGPV GTEPTVETLE PLGVLFPSTT KKRKKPKGKE TFEPEDKTVK QEQINTEPLE DTVLSPTKKR KRQKGTEGME PEEGVTVESQ PQVKVEPLEE AIPLPPTKKR KKEKGQMAMM EPGTEAMEPV EPEMKPLESP GGTMAPQQPE GAKPQAQAAL AAPKKKTKKE KQQDATVEPE TEVVGPELPD DLEPQAAPTS TKKKKKKKER GHTVTEPIQP LEPELPGEGQ PEARATPGST KKRKKQSQES RMPETVPQEE MPGPPLNSES GEEAPTGRDK KRKQQQQQPV |
| 预测分子量 | 54,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于CD3ε重组蛋白的3篇模拟参考文献示例，涵盖结构、功能及应用研究方向：
1. **文献名称**：*Structural Insights into the CD3ε Subunit of the T-Cell Receptor Complex*
**作者**：Garcia, K.C. et al.
**摘要**：该研究通过X射线晶体学解析了CD3ε胞外域的结构，揭示了其与TCR复合物中其他亚基(如CD3γ/δ)的相互作用界面，为理解T细胞信号传导的分子机制提供了结构基础。
2. **文献名称**：*CD3ε-Based Bispecific Antibodies Enhance Anti-Tumor T Cell Activation*
**作者**：Smith, R.P. et al.
**摘要**：本文开发了一种以CD3ε胞外域重组蛋白为核心的双特异性抗体，通过靶向肿瘤抗原(如CD19)和T细胞CD3ε，显著增强T细胞对癌细胞的杀伤活性，并在小鼠模型中验证了其治疗潜力。
3. **文献名称**：*Functional Analysis of CD3ε Chimeric Antigen Receptors in Adoptive Immunotherapy*
**作者**：Wang, L. et al.
**摘要**：研究团队将CD3ε重组蛋白与肿瘤特异性单链抗体融合，构建新型CAR-T细胞，实验表明该设计可优化T细胞激活阈值，在体外和体内模型中均显示出更强的抗肿瘤效果。
4. **文献名称**：*CD3ε Mutagenesis Reveals Critical Residues for T Cell Signaling*
**作者**：Lee, H. et al.
**摘要**：通过定点突变筛选CD3ε胞内域的关键功能位点，发现特定磷酸化位点(如ITAM基序)对其介导下游信号通路(如NF-κB)至关重要，为靶向T细胞活化的药物设计提供理论依据。
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注：以上内容为模拟文献，实际研究需参考真实发表的论文(可通过PubMed或Google Scholar搜索关键词“CD3ε recombinant protein”“TCR signaling”等获取)。
CD3eAP (CD3 epsilon Alkaline Phosphatase) is a recombinant fusion protein designed to study T cell receptor (TCR) signaling and immune synapse formation. The CD3ε chain is a critical component of the TCR complex, which plays a central role in antigen recognition and T cell activation. It forms part of the CD3 heterodimer (CD3γε/δε) that associates with the TCR α/β chains to transduce intracellular signals upon MHC-antigen engagement.
The CD3eAP construct typically fuses the extracellular domain of human CD3ε to the C-terminal enzymatic domain of human placental alkaline phosphatase (AP). This design allows the protein to retain the antigen-binding specificity of CD3ε while enabling sensitive detection via AP’s enzymatic activity, which catalyzes colorimetric or chemiluminescent reactions. As a soluble, single-chain protein, CD3eAP mimics the extracellular portion of the native CD3ε subunit, making it a valuable tool for probing TCR-ligand interactions without requiring membrane-bound complexes.
Applications include in vitro binding assays to study TCR-pMHC interactions, screening therapeutic antibodies targeting CD3 (e.g., bispecific T cell engagers), and visualizing immune synapses in fixed or live-cell imaging. Its soluble nature simplifies experimental workflows compared to full TCR-expressing cells. CD3eAP has been particularly useful in characterizing low-affinity TCR-pMHC interactions and validating CD3-targeting immunotherapies. Commercial and academic variants may differ in tag configurations (e.g., Fc fusions) to accommodate specific detection methods or binding studies.
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