| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PGGT1b |
| Uniprot No | P53609 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-377aa |
| 氨基酸序列 | MAATEDERLA GSGEGERLDF LRDRHVRFFQ RCLQVLPERY SSLETSRLTI AFFALSGLDM LDSLDVVNKD DIIEWIYSLQ VLPTEDRSNL NRCGFRGSSY LGIPFNPSKA PGTAHPYDSG HIAMTYTGLS CLVILGDDLS RVNKEACLAG LRALQLEDGS FCAVPEGSEN DMRFVYCASC ICYMLNNWSG MDMKKAITYI RRSMSYDNGL AQGAGLESHG GSTFCGIASL CLMGKLEEVF SEKELNRIKR WCIMRQQNGY HGRPNKPVDT CYSFWVGATL KLLKIFQYTN FEKNRNYILS TQDRLVGGFA KWPDSHPDAL HAYFGICGLS LMEESGICKV HPALNVSTRT SERLLDLHQS WKTKDSKQCS ENVHIST |
| 预测分子量 | 42,3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PGGT1b(蛋白香叶酰基转移酶I β亚基)重组蛋白研究的示例参考文献(注：以下文献为示例性内容，实际文献需通过学术数据库检索获取)：
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1. **文献名称**：*"Expression and Characterization of Recombinant PGGT1b for Farnesylation Studies"*
**作者**：Zhang Y. et al.
**摘要**：研究报道了通过大肠杆菌系统成功表达并纯化重组PGGT1b蛋白，验证其催化Ras蛋白香叶酰化修饰的活性，为开发靶向异戊二烯化通路的抑制剂提供实验基础。
2. **文献名称**：*"Role of PGGT1b in Oncogenic Signaling and Cancer Therapeutics"*
**作者**：Smith J.L. et al.
**摘要**：探讨PGGT1b在Ras信号通路中的关键作用，发现其重组蛋白可用于高通量筛选小分子抑制剂，并证明其抑制可阻断肿瘤细胞增殖。
3. **文献名称**：*"Structural Insights into PGGT1b Catalytic Mechanism by Cryo-EM"*
**作者**：Bracha S. et al.
**摘要**：通过冷冻电镜解析重组PGGT1b蛋白的三维结构，揭示其与底物结合的分子机制，为设计特异性调节剂提供结构生物学依据。
4. **文献名称**：*"PGGT1b Knockout and Rescue Models in Neurodegenerative Disease"*
**作者**：Johnson R.T. et al.
**摘要**：利用重组PGGT1b蛋白恢复基因敲除细胞的香叶酰化功能，研究其在tau蛋白异常修饰与阿尔茨海默病病理中的潜在关联。
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**建议**：实际文献可通过PubMed、Google Scholar等平台以关键词“PGGT1B recombinant protein”“geranylgeranyltransferase”检索，重点关注蛋白功能、结构或疾病应用方向的研究。
**Background of PGGT1b Recombinant Protein**
PGGT1b (Protein Geranylgeranyltransferase Type I Subunit Beta), also known as GGTase I β-subunit, is a critical enzyme involved in post-translational protein modification. It forms a heterodimeric complex with the α-subunit (PGGT1a) to catalyze the geranylgeranylation of target proteins—a lipid modification process essential for membrane localization and functional activation of small GTPases, particularly those in the Rho and Rac families. This modification involves attaching a 20-carbon geranylgeranyl isoprenoid group to cysteine residues within C-terminal CAAX motifs, enabling interactions with cellular membranes and participation in signaling pathways regulating cell proliferation, cytoskeletal organization, and vesicular trafficking.
The recombinant PGGT1b protein is generated using biotechnological platforms (e.g., *E. coli* or mammalian expression systems) to produce a purified, functional form of the subunit for research. Its recombinant version retains enzymatic activity when combined with PGGT1a, facilitating studies on geranylgeranylation mechanisms and substrate specificity. Dysregulation of PGGT1b is implicated in diseases such as cancer, neurodegeneration, and immune disorders, as aberrant GTPase activity contributes to uncontrolled cell growth or impaired signaling.
Research on recombinant PGGT1b aids in developing inhibitors targeting geranylgeranylation, with potential therapeutic applications. For example, blocking this process may disrupt oncogenic GTPase signaling in tumors. Additionally, structural studies using recombinant PGGT1b provide insights into its zinc-binding motifs and substrate-binding domains, advancing drug design. Overall, PGGT1b recombinant protein serves as a vital tool for dissecting post-translational modification pathways and exploring therapeutic strategies for related diseases.
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