| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | FEz1 |
| Uniprot No | Q99689 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-392aa |
| 氨基酸序列 | MEAPLVSLDE EFEDLRPSCS EDPEEKPQCF YGSSPHHLED PSLSELENFS SEIISFKSME DLVNEFDEKL NVCFRNYNAK TENLAPVKNQ LQIQEEEETL QDEEVWDALT DNYIPSLSED WRDPNIEALN GNCSDTEIHE KEEEEFNEKS ENDSGINEEP LLTADQVIEE IEEMMQNSPD PEEEEEVLEE EDGGETSSQA DSVLLQEMQA LTQTFNNNWS YEGLRHMSGS ELTELLDQVE GAIRDFSEEL VQQLARRDEL EFEKEVKNSF ITVLIEVQNK QKEQRELMKK RRKEKGLSLQ SSRIEKGNQM PLKRFSMEGI SNILQSGIRQ TFGSSGTDKQ YLNTVIPYEK KASPPSVEDL QMLTNILFAM KEDNEKVPTL LTDYILKVLC PT |
| 预测分子量 | 45,1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于FEz1重组蛋白的3条参考文献示例(注：内容为模拟概括，非真实文献)：
1. **文献名称**: *FEz1 regulates axonal growth via interaction with kinesin light chain in neuronal cells*
**作者**: Yamamoto N, et al.
**摘要**: 本研究通过体外重组FEz1蛋白，揭示了其与驱动蛋白KLC的结合机制，证明FEz1-KLC复合物对轴突生长的正向调控作用，为神经发育机制提供了新见解。
2. **文献名称**: *Expression and purification of recombinant FEz1 protein in E. coli for structural studies*
**作者**: Li X, Wang Y.
**摘要**: 报道了一种高效的大肠杆菌表达系统，用于可溶性FEz1重组蛋白的制备，并通过质谱和圆二色谱验证其正确折叠，为后续功能研究提供材料基础。
3. **文献名称**: *FEz1 dysfunction alters dopamine receptor trafficking in Parkinson's disease models*
**作者**: Smith J, et al.
**摘要**: 利用FEz1重组蛋白进行体外结合实验，发现其与多巴胺受体D2的运输相关，异常FEz1表达导致受体分布紊乱，提示其在帕金森病理中的潜在作用。
(注：以上为示例性内容，实际文献需通过学术数据库检索确认。)
FEz1 (Fasciculation and Elongation Protein Zeta-1) is a neuronal cytosolic protein implicated in axonal guidance, synaptic plasticity, and intracellular transport. Initially identified in rodents, it is highly expressed in the nervous system, particularly during developmental stages. FEz1 interacts with molecular motors like kinesin-1. regulating cargo trafficking along microtubules, which is critical for neuronal polarization and neurotransmitter release. Structurally, it contains coiled-coil domains that mediate protein-protein interactions and a conserved N-terminal region essential for its function. Dysregulation of FEz1 has been linked to neurodevelopmental disorders and neurodegenerative diseases, including schizophrenia and Alzheimer’s disease, highlighting its role in maintaining neural network integrity.
Recombinant FEz1 protein is engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional FEz1 for mechanistic studies. Researchers employ it to dissect FEz1’s binding partners, phosphorylation dynamics, and its influence on cytoskeletal organization. Its recombinant form enables *in vitro* assays, such as microtubule-binding experiments, pull-down assays, and cell culture models, to explore how FEz1 modulates axonal transport or synaptic vesicle dynamics. Additionally, FEz1 recombinant protein serves as a tool to screen therapeutic compounds targeting FEz1-related pathways, offering potential avenues for treating neurological disorders. Studies using recombinant FEz1 have also clarified its dual role in promoting or inhibiting neurite outgrowth, depending on cellular context and post-translational modifications. Overall, FEz1 recombinant protein is pivotal for unraveling molecular mechanisms in neurobiology and disease pathogenesis.
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