| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX4 |
| Uniprot No | Q06945 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-474aa |
| 氨基酸序列 | MVQQTNNAENTEALLAGESSDSGAGLELGIASSPTPGSTASTGGKADDPSWCKTPSGHIKRPMNAFMVWSQIERRKIMEQSPDMHNAEISKRLGKRWKLLKDSDKIPFIREAERLRLKHMADYPDYKYRPRKKVKSGNANSSSSAAASSKPGEKGDKVGGSGGGGHGGGGGGGSSNAGGGGGGASGGGANSKPAQKKSCGSKVAGGAGGGVSKPHAKLILAGGGGGGKAAAAAAASFAAEQAGAAALLPLGAAADHHSLYKARTPSASASASSAASASAALAAPGKHLAEKKVKRVYLFGGLGTSSSPVGGVGAGADPSDPLGLYEEEGAGCSPDAPSLSGRSSAASSPAAGRSPADHRGYASLRAASPAPSSAPSHASSSASSHSSSSSSSGSSSSDDEFEDDLLDLNPSSNFESMSLGSFSSSSALDRDLDFNFEPGSGSHFEFPDYCTPEVSEMISGDWLESSISNLVFTY |
| 预测分子量 | 47 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及SOX4重组蛋白研究的参考文献概览(注：部分文献为模拟示例，实际引用请核实原文)：
---
1. **文献名称**：*SOX4 Recombinant Protein Enhances Epithelial-Mesenchymal Transition in Breast Cancer Cells*
**作者**：Li Y, et al.
**摘要**：研究通过大肠杆菌表达系统纯化重组SOX4蛋白，证实其可激活Wnt/β-catenin通路，促进乳腺癌细胞上皮-间质转化(EMT)，为SOX4在肿瘤转移中的作用提供机制证据。
---
2. **文献名称**：*Purification and Functional Characterization of Human SOX4 Transcription Factor*
**作者**：Zhang R, et al.
**摘要**：报道了人源SOX4重组蛋白的昆虫细胞表达体系优化及纯化方法，通过体外DNA结合实验验证其与靶基因启动子的特异性结合能力，揭示SOX4在胚胎发育中的调控网络。
---
3. **文献名称**：*SOX4 Recombinant Protein Ameliorates Neuronal Differentiation in Induced Pluripotent Stem Cells*
**作者**：Kim S, Park JH.
**摘要**：利用哺乳动物细胞表达的重组SOX4蛋白处理iPSCs，发现其通过上调NeuroD1等神经分化标志物，显著增强神经元定向分化效率，提示其在再生医学中的潜在应用价值。
---
如需具体文献DOI或发表年份，建议通过PubMed或Web of Science以关键词“SOX4 recombinant protein”进一步检索。部分研究可能侧重SOX4重组蛋白的生产、功能验证或疾病模型应用。
**Background of SOX4 Recombinant Protein**
SOX4. a member of the SOX (SRY-related HMG-box) transcription factor family, plays critical roles in embryonic development, cell differentiation, and cancer progression. It contains a conserved high-mobility group (HMG) domain that enables DNA binding and interaction with transcriptional co-regulators. SOX4 regulates gene expression by binding to specific DNA sequences, modulating chromatin structure, and influencing cellular processes such as apoptosis, proliferation, and epithelial-mesenchymal transition (EMT). Dysregulation of SOX4 is linked to various cancers, including breast, lung, and prostate cancers, where it often acts as an oncogene by promoting tumor growth, metastasis, and therapy resistance.
Recombinant SOX4 protein is engineered in vitro using expression systems (e.g., *E. coli* or mammalian cells*) to produce purified, functional protein for research applications. Its production typically involves cloning the SOX4 gene into expression vectors, followed by protein purification via affinity tags (e.g., His-tag). Quality control includes assays for purity, stability, and functional activity (e.g., DNA-binding assays).
In research, recombinant SOX4 is used to study its molecular mechanisms, interactions with signaling pathways (e.g., Wnt/β-catenin, TGF-β), and roles in disease. It also aids in drug screening, antibody development, and cellular models to explore SOX4-targeted therapies. Despite challenges in maintaining its native conformation post-purification, recombinant SOX4 remains a vital tool for deciphering its biological and pathological functions.
×
