| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb9 |
| Uniprot No | Q9Y5E1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-797aa |
| 氨基酸序列 | MKTRGFSFPRQRQVLFLFLFWGVSLAGSGFGRYSVTEETEKGSFVVNLAKDLGLAEGELAARGTRVVSDDNKQYLLLDSHTGNLLTNEKLDREKLCGPKEPCMLYFQILMDDPFQIYRAELRVRDINDHSPVFRHKEMVLKISENTAEGTAFRLERAQDPDEGHNSIQNYTISSNSFFHIKISGSDEGMIYPELVLDKALDREEQEELSLTLTALDGGSPSRSGTSTIRIVVLDVNDNVPQFAQALYETQAPENSPVGSLIVKVSAGDADSGVNAEVSYSFFDASEDILTTFQINPFSGEIFLRELLDYELVNSYKINIQAMDGGGLSARCTVLIKVLDSNDNPPELIISSLSNSVAENSPGIVLAVFKIKDRDSGENGKTICYVQDNLPFFLKPSVDNFYILMTEGALDRESKAEYNITITVTDLGTPRLKTEHSITLQVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQDPHLPLASLVSINADNGHLFALRSLDYEALQAFDFRVGASDRGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADLLTVYLVVALASVSSLFLLSVLLFVAVRLCRRSRAASVGRCSVPEGPFPGHLVDVSGTGTLFQSYQYEVCLTGGSETGEFKFLKPITPHLPPHRGGKEIEENSTLPNSFGFNY |
| 预测分子量 | 87 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHb9重组蛋白的模拟参考文献示例(实际文献需通过学术数据库查询)：
---
1. **文献名称**: *Structural characterization of recombinant PCDHb9 and its role in neural adhesion*
**作者**: Suzuki, T., et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了PCDHb9重组蛋白，通过X射线晶体学解析其胞外结构域的三维结构，揭示了其钙离子依赖性同源二聚化机制，提示其在神经细胞粘附中的潜在作用。
2. **文献名称**: *PCDHb9 recombinant protein modulates synaptic connectivity in vitro*
**作者**: Kim, J., & Zhang, Y.
**摘要**: 利用哺乳动物细胞系统表达PCDHb9重组蛋白，发现其可促进体外培养的神经元突触形成，并通过阻断实验证明其胞内结构域与WAVE复合体的相互作用可能介导此功能。
3. **文献名称**: *Dysregulation of PCDHb9 in autism spectrum disorder: Functional rescue using recombinant protein*
**作者**: Smith, L., et al.
**摘要**: 在ASD患者类脑器官模型中观察到PCDHb9表达下调，通过添加重组PCDHb9蛋白部分恢复了神经元迁移缺陷，提示其作为治疗靶点的潜力。
4. **文献名称**: *Optimized expression and purification of PCDHb9 for high-throughput screening*
**作者**: Chen, X., et al.
**摘要**: 开发了一种基于昆虫细胞-杆状病毒系统的PCDHb9重组蛋白高效表达及纯化方案，为后续药物筛选或抗体开发提供了可靠方法。
---
**注意**：以上为模拟示例，实际研究中PCDHb9相关文献可能较少。建议通过 **PubMed** 或 **Google Scholar** 搜索最新文献，关键词可尝试：`"PCDHb9 recombinant"` 或 `"Protocadherin beta 9 expression"`。
PCDHb9 (Protocadherin Beta-9) is a member of the protocadherin β gene cluster, a subgroup of the cadherin superfamily known for mediating calcium-dependent cell-cell adhesion and signaling. Protocadherins play critical roles in neural development, including neuronal circuit formation, synaptic plasticity, and axonal guidance. The β-cluster protocadherins, encoded by a tandem array of genes, are particularly implicated in establishing cellular diversity and specificity in the central nervous system. PCDHb9. like other protocadherins, features an extracellular domain with cadherin-like repeats, a transmembrane domain, and a cytoplasmic region that may interact with intracellular signaling molecules. Its expression is enriched in the brain, suggesting a specialized function in neural connectivity, though its precise biological mechanisms remain under investigation.
Recombinant PCDHb9 protein is engineered to enable functional and structural studies, bypassing challenges associated with endogenous protein purification. Typically produced in heterologous systems like mammalian cells or insect cells, the recombinant form retains post-translational modifications essential for its adhesive properties. Researchers utilize it to explore PCDHb9's role in cell adhesion assays, neurite outgrowth models, or receptor-ligand interaction studies. Dysregulation of protocadherins has been linked to neurodevelopmental disorders, psychiatric conditions, and cancers, positioning PCDHb9 as a potential biomarker or therapeutic target. Recombinant PCDHb9 also aids in antibody development for diagnostic applications. Current research focuses on deciphering its isoform-specific interactions and contributions to neural network patterning, offering insights into both fundamental neurobiology and disease pathways.
×
