| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDH12 |
| Uniprot No | Q9NPG4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 25-718aa |
| 氨基酸序列 | MASMTGGQQMGRGHHHHHHGNLYFQGGEFQEVTTLTVKYQVSEEVPSGTV IGKLSQELGREERRRQAGAAFQVLQLPQALPIQVDSEEGLLSTGRRLDRE QLCRQWDPCLVSFDVLATGDLALIHVEIQVLDINDHQPRFPKGEQELEIS ESASLRTRIPLDRALDPDTGPNTLHTYTLSPSEHFALDVIVGPDETKHAE LIVVKELDREIHSFFDLVLTAYDNGNPPKSGTSLVKVNVLDSNDNSPAFA ESSLALEIQEDAAPGTLLIKLTATDPDQGPNGEVEFFLSKHMPPEVLDTF SIDAKTGQVILRRPLDYEKNPAYEVDVQARDLGPNPIPAHCKVLIKVLDV NDNIPSIHVTWASQPSLVSEALPKDSFIALVMADDLDSGNNGLVHCWLSQ ELGHFRLKRTNGNTYMLLTNATLDREQWPKYTLTLLAQDQGLQPLSAKKQ LSIQISDINDNAPVFEKSRYEVSTRENNLPSLHLITIKAHDADLGINGKV SYRIQDSPVAHLVAIDSNTGEVTAQRSLNYEEMAGFEFQVIAEDSGQPML ASSVSVWVSLLDANDNAPEVVQPVLSDGKASLSVLVNASTGHLLVPIETP NGLGPAGTDTPPLATHSSRPFLLTTIVARDADSGANGEPLYSIRSGNEAH LFILNPHTGQLFVNVTNASSLIGSEWELEIVVEDQGSPPLQTRALLRVMF VTSVDHLRDSARKPGALSMSMLT |
| 预测分子量 | 79 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇关于PCDH12重组蛋白的模拟参考文献(注：以下内容为示例，非真实存在的文献，建议通过学术数据库获取真实文献)：
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1. **标题**: *Recombinant PCDH12 Expression and Its Role in Endothelial Cell Adhesion*
**作者**: Smith A, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了PCDH12重组蛋白，发现其能够增强内皮细胞的粘附能力，并调节血管生成相关信号通路。
2. **标题**: *Structural Characterization of PCDH12 and Its Interaction with Membrane Lipids*
**作者**: Chen L, et al.
**摘要**: 利用重组PCDH12蛋白解析了其胞外结构域的三维构象，揭示了其与细胞膜磷脂的特异性结合，为理解原钙黏蛋白家族在细胞膜动态调控中的作用提供依据。
3. **标题**: *PCDH12 Recombinant Protein Attenuates Inflammatory Response in Placental Trophoblasts*
**作者**: Gupta R, et al.
**摘要**: 在胎盘滋养层细胞模型中，重组PCDH12蛋白显著抑制了炎症因子IL-6和TNF-α的释放，提示其在妊娠相关炎症疾病中的潜在治疗价值。
4. **标题**: *Functional Analysis of PCDH12 in Neural Circuit Development Using Recombinant Protein*
**作者**: Tanaka K, et al.
**摘要**: 通过体外重组PCDH12蛋白的神经突触导向实验，证实其参与轴突导向和突触可塑性，可能影响神经网络的早期发育。
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建议通过 **PubMed**、**Web of Science** 或 **Google Scholar** 检索真实文献，关键词可组合：`PCDH12 recombinant protein`、`PCDH12 expression`、`protocadherin-12 function`。
**Background of PCDH12 Recombinant Protein**
Protocadherin-12 (PCDH12), a member of the cadherin superfamily, is a calcium-dependent cell adhesion protein primarily involved in cell-cell interactions and tissue morphogenesis. It belongs to the non-clustered δ2-protocadherin subgroup, characterized by extracellular cadherin repeats and a cytoplasmic domain lacking conserved signaling motifs. PCDH12 is expressed in endothelial cells, the placenta, and the developing nervous system, where it plays critical roles in vascular development, blood-brain barrier formation, and placental angiogenesis.
Studies highlight its importance in embryogenesis, particularly in vascular and neural network assembly. PCDH12 interacts with vascular endothelial growth factor (VEGF) signaling pathways, influencing endothelial cell migration and tube formation. Dysregulation of PCDH12 has been linked to pathological conditions, including preeclampsia, intrauterine growth restriction, and cancer metastasis, underscoring its role in maintaining vascular integrity and tissue homeostasis.
Recombinant PCDH12 protein is engineered to study its structural and functional properties in vitro. Produced using expression systems like mammalian cells (e.g., HEK293) or bacteria, the recombinant protein retains key domains for ligand binding and cellular adhesion. It is purified via affinity tags (e.g., His-tag) and validated for bioactivity through cell adhesion or migration assays.
Research applications include investigating PCDH12’s role in endothelial barrier function, tumor angiogenesis, and placental development. It also serves as a tool for generating antibodies or screening therapeutic agents targeting vascular or neurological disorders. By elucidating PCDH12’s molecular interactions, researchers aim to develop strategies for treating diseases linked to vascular dysfunction or abnormal cell adhesion.
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