| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIAS4 |
| Uniprot No | Q8N2W9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-510aa |
| 氨基酸序列 | AAELVEAKN MVMSFRVSDL QMLLGFVGRS KSGLKHELVT RALQLVQFDC SPELFKKIKE LYETRYAKKN SEPAPQPHRP LDPLTMHSTY DRAGAVPRTP LAGPNIDYPV LYGKYLNGLG RLPAKTLKPE VRLVKLPFFN MLDELLKPTE LVPQNNEKLQ ESPCIFALTP RQVELIRNSR ELQPGVKAVQ VVLRICYSDT SCPQEDQYPP NIAVKVNHSY CSVPGYYPSN KPGVEPKRPC RPINLTHLMY LSSATNRITV TWGNYGKSYS VALYLVRQLT SSELLQRLKT IGVKHPELCK ALVKEKLRLD PDSEIATTGV RVSLICPLVK MRLSVPCRAE TCAHLQCFDA VFYLQMNEKK PTWMCPVCDK PAPYDQLIID GLLSKILSEC EDADEIEYLV DGSWCPIRAE KERSCSPQGA ILVLGPSDAN GLLPAPSVNG SGALGSTGGG GPVGSMENGK PGADVVDLTL DSSSSSEDEE EEEEEEEDED EEGPRPKRRC PFQKGLVPAC |
| 预测分子量 | 56 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇涉及PIAS4重组蛋白研究的参考文献摘要：
1. **"PIAS4-mediated sumoylation of DNA-dependent protein kinase regulates non-homologous end-joining repair of DNA double-strand breaks"**
*作者：Yuan, T., Chen, J., & Wu, C.*
摘要：研究报道了重组表达PIAS4蛋白通过SUMO化修饰调控DNA-PK复合体的活性，促进DNA双链断裂修复的分子机制。
2. **"Structural basis for the SUMO E3 ligase activity of PIAS4"**
*作者：Liu, X., Yang, Y., & Pruneda, J.N.*
摘要：通过X射线晶体学解析重组人源PIAS4蛋白的SP-RING结构域结构，揭示了其作为SUMO E3连接酶的催化机理及底物识别特异性。
3. **"PIAS4 inhibits NF-κB activity through covalent SUMO modification of p65 subunit"**
*作者：Huang, Y., Liu, H., & Shu, H.B.*
摘要：利用重组PIAS4蛋白进行体外SUMOylation实验，证明其通过直接SUMO化p65抑制NF-κB信号通路，调节炎症反应。
注：以上文献为模拟示例，实际引用建议通过PubMed/Web of Science检索最新论文。研究焦点多集中在PIAS4的SUMO E3连接酶功能及其在DNA修复、信号转导中的作用。
PIAS4 (Protein Inhibitor of Activated STAT 4) is a member of the PIAS family of proteins, which function as regulators of transcription factors and chromatin-modifying enzymes through SUMO (Small Ubiquitin-like Modifier) post-translational modification. Originally identified as a suppressor of STAT (Signal Transducer and Activator of Transcription) signaling, PIAS4 modulates diverse cellular processes, including inflammation, DNA damage response, and immune regulation. It acts as an E3 SUMO ligase, facilitating the attachment of SUMO proteins to target substrates, thereby altering their activity, localization, or interactions.
Structurally, PIAS4 contains a conserved N-terminal SAP (SAF-A/B, Acinus, PIAS) domain involved in DNA binding, a PINIT motif for subnuclear localization, and a C-terminal SP-RING domain critical for SUMO ligase activity. Its interactions with key signaling molecules—such as NF-κB, p53. and steroid receptors—highlight its role in balancing pro- and anti-inflammatory pathways, apoptosis, and hormone-dependent gene expression. Dysregulation of PIAS4 has been implicated in cancer, autoimmune diseases, and neurodegenerative disorders, making it a potential therapeutic target.
Recombinant PIAS4 protein is engineered for in vitro studies to dissect its biochemical mechanisms, screen inhibitors, or analyze SUMOylation cascades. Produced via bacterial or mammalian expression systems, it retains SUMO ligase activity and can be tagged (e.g., His, GST) for purification or detection. Researchers utilize it to explore how PIAS4-mediated SUMOylation fine-tunes cellular responses to stress, cytokines, or DNA damage, offering insights into disease pathogenesis and drug development. Its application extends to structural studies, enzyme kinetics, and reconstituting SUMOylation pathways in cell-free systems.
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