| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHgA6 |
| Uniprot No | Q9Y5G7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-932aa |
| 氨基酸序列 | MAPPQRHPQRSEQVLLLTLLGTLWGAAAAQIRYSIPEELEKGSFVGNIVKDLGLEPQELAEHGVRIVSRGRMQLFSLNPRNGSLVTAGRIDREELCAQSPRCLVSFNILVEDKLNLYPVEVEIVDINDNTPRFLKEELEVKILENAAPSSRFPLMEVYDPDVGMNSLQGFKLSGNSHFSVDVQSEAHGPKYPELVLEGTLDREGEAVYRLVLTAMDGGDPVRSSVAQILVTVLDVNDNTPMFTQPVYRVSVPENLPVGTPVLAVTATDQDEGVHGEVTYSFVKITEKISQIFCLNVLTGEISTSANLDYEDSSFYELGVEARDGPGLRDRAKVLITILDVNDNVPEVVVTSGSRTIAESAPPGTVIALFQVFDRDSGLNGLVTCSIPRSLPFELEKSVGNYYRLVTNAALDREEVFLYNITVTATDKGTPPLSTETIISLNVADTNDNPPTFPHSSYSVYVLENNPRGASIFSVNALDPDVDQNAQVSYSLAEDTLQGAPLSSYVSINSDTGILYALRSFDYEQLRDLQLWVTASDSGDPPLSSNVSLSLFVLDQNDNAPEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYRLLKASEPGLFSVGLHTGEVRTARALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADRIPDILADLGSLEPSAKPNDSDLTLYLVVAVAAVSCVFLAFVIVLLALRLQRWHKSRLLQASGGGLASMPGSHFVGVEGVRAFLQTYSHEVSLTADSRKSHLIFPQPNYADTLINQESYEKSEPLLITQDLLETKGEPRQLQQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 预测分子量 | 100 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是模拟生成的关于PCDHgA6重组蛋白的参考文献示例(实际文献需通过学术数据库验证)：
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1. **文献名称**: *Structural characterization of recombinant PCDHgA6 extracellular domain and its role in neuronal adhesion*
**作者**: Smith, J. et al.
**摘要**: 本研究通过大肠杆菌系统重组表达了PCDHgA6的胞外域蛋白，利用X射线晶体学解析其三维结构，发现其钙离子依赖性同源二聚化特性，并验证其在神经元细胞黏附中的关键作用。
2. **文献名称**: *Functional analysis of PCDHgA6 in synaptic plasticity using CRISPR/Cas9 and recombinant protein rescue*
**作者**: Li, X. et al.
**摘要**: 通过CRISPR敲除小鼠模型结合重组PCDHgA6蛋白回补实验，证明PCDHgA6通过调控NMDA受体稳定性影响突触可塑性，其重组蛋白可部分恢复突触传递缺陷。
3. **文献名称**: *PCDHgA6 recombinant protein as a potential biomarker in autism spectrum disorder*
**作者**: Garcia, R. et al.
**摘要**: 开发基于重组PCDHgA6蛋白的ELISA检测方法，发现自闭症患者血清中PCDHgA6自身抗体水平显著升高，提示其可能与神经发育异常相关。
4. **文献名称**: *High-yield purification of recombinant PCDHgA6 for antibody production and epitope mapping*
**作者**: Wang, Y. et al.
**摘要**: 优化哺乳动物细胞表达系统实现PCDHgA6重组蛋白的高效纯化，利用该蛋白成功制备单克隆抗体，并鉴定出与神经退行性疾病相关的特异性表位。
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注：以上为模拟生成的示例，实际文献需通过PubMed、Google Scholar等平台检索关键词(如"PCDHgA6 recombinant"、"protocadherin gamma A6")获取。如需具体文献协助，可提供更详细的研究方向或数据库访问权限。
**Background of PCDHgA6 Recombinant Protein**
The PCDHgA6 (Protocadherin gamma subfamily A6) recombinant protein is a engineered version of a cell adhesion molecule belonging to the protocadherin gamma gene cluster, part of the cadherin superfamily. Protocadherins are transmembrane proteins critical for establishing neuronal connectivity, synaptic specificity, and neural circuit formation during brain development. The gamma subfamily (PCDHγ) consists of 22 isoforms in humans, generated through alternative splicing, with PCDHgA6 being one of the key isoforms. These proteins mediate homophilic interactions, enabling cell-cell recognition and adhesion, which are essential for neuronal survival, axon guidance, and dendritic self-avoidance.
Structurally, PCDHgA6 contains extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail. The recombinant form is typically produced in vitro using expression systems (e.g., mammalian, insect, or bacterial cells) to enable functional studies. It retains the extracellular domain’s binding capacity, allowing researchers to investigate its role in neural development, synaptic plasticity, and disease mechanisms.
Research on PCDHgA6 is linked to neurodevelopmental disorders, including autism spectrum disorders (ASD) and schizophrenia, as mutations or dysregulation in protocadherin genes disrupt neural network formation. Recombinant PCDHgA6 facilitates studies on its interaction partners, signaling pathways, and potential therapeutic targeting. Its applications span in vitro assays, disease modeling, and drug screening, offering insights into brain development and neurological pathologies.
Overall, PCDHgA6 recombinant protein serves as a vital tool for decoding the molecular basis of neural connectivity and associated diseases.
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