| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb14 |
| Uniprot No | Q9Y5E9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-798aa |
| 氨基酸序列 | MEIRGALDLRKRQVLIFLVLLGLSRAGTESAHYSVAEETEIGSFVANLARDLGLGVEELSSREARVVSDDNKKYLHLDLLTGNLLLNEKLDRDELCGSTEPCVLHFQVVLENPLQFFRFELCVKDINDHSPTFLDKEILIKISEGTTVGATFLMESAQDLDVGSNSLQNYTISPNSHFYIKIPDSSDRKIYPELVLDRALDYEQEAELRLTLTAVDGGSPPKSGTTLVLIKVLDINDNAPEFPQSLYEVQVPEDRPLGSWIATISAKDLDAGNYGKISYTFFHASEDIRKTFEINPISGEVNLRSPLDFEVIQSYTINIQATDGGGLSGKCTLLVKVMDINDNPPEVTISSITKRIPENASETLVALFSILDQDSGDNGRMICSIQDNLPFFLKPTFKNFFTLVSEKALDRESQAEYNITITVTDLGTPRLKTEYNITVLLSDVNDNAPTFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVNYSLLPPQDRHLPLASLVSINADNGHLFALRSLDYEALQEFEFRVGATDRGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAASVGRCSVPEGPFPGHLVDVSGTGTLSQSYQYEVCLTGGSGTNEFKFLKPIIPNFQVHDTGRNMGEIENFRNSFGLNIQ |
| 预测分子量 | 87,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHb14重组蛋白的模拟参考文献示例(请注意，以下内容为虚构，实际文献需通过学术数据库查询)：
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1. **文献名称**: *Recombinant PCDHb14 promotes synaptic formation in vitro*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化了PCDHb14重组蛋白，发现其能显著增强体外培养神经元突触的形成，提示其在神经发育中的潜在作用。
2. **文献名称**: *Structural characterization of PCDHb14 extracellular domain*
**作者**: Lee S, et al.
**摘要**: 利用昆虫细胞表达系统获得PCDHb14胞外域重组蛋白，通过X射线晶体学解析其三维结构，揭示了其钙离子结合位点及同源二聚化机制。
3. **文献名称**: *PCDHb14 recombinant protein inhibits tumor cell migration*
**作者**: Kim J, et al.
**摘要**: 研究发现，在乳腺癌细胞模型中，外源性添加PCDHb14重组蛋白可通过调控Wnt信号通路抑制癌细胞迁移，表明其可能的肿瘤抑制功能。
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**建议**：实际文献可通过PubMed、Google Scholar等平台，以关键词“PCDHb14 recombinant”或“Protocadherin beta 14 expression”检索。部分研究可能侧重基因家族功能或技术方法(如蛋白纯化)，可结合具体研究目标筛选。
PCDHb14 (Protocadherin Beta-14) is a member of the protocadherin-beta subfamily within the cadherin superfamily, a group of calcium-dependent cell adhesion molecules critical for neural development and synaptic specificity. Protocadherins are transmembrane proteins characterized by extracellular cadherin repeats that mediate homophilic or heterophilic interactions, playing pivotal roles in establishing neuronal connectivity, circuit assembly, and self-avoidance in the nervous system. The PCDHB gene cluster, located on human chromosome 5q31. encodes 16 beta-type protocadherins (PCDHb1 to PCDHb16), including PCDHb14. These proteins exhibit combinatorial diversity in their extracellular domains, enabling precise cell recognition and synaptic targeting during brain development.
Recombinant PCDHb14 protein is typically produced in vitro using expression systems like mammalian cells or E. coli to study its structural and functional properties. Researchers employ it to investigate calcium-dependent adhesion mechanisms, intracellular signaling pathways, and its role in neural network formation. Emerging studies suggest PCDHb14 may influence axon guidance, dendritic arborization, and synaptic plasticity, with potential implications in neurodevelopmental disorders such as autism spectrum disorders or schizophrenia. Structural studies focus on its extracellular cadherin domains responsible for selective homophilic binding, while functional assays explore interactions with cytoplasmic proteins like tyrosine kinases or cytoskeletal regulators. Challenges in PCDHb14 research include resolving its isoform-specific functions within the highly homologous protocadherin family and characterizing post-translational modifications affecting its activity. Recombinant protein tools are essential for developing targeted therapies or diagnostic biomarkers for neurological conditions linked to protocadherin dysregulation.
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