| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RIMS2 |
| Uniprot No | Q9UQ26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1411aa |
| 氨基酸序列 | MSAPVGPRGRLAPIPAASQPPLQPEMPDLSHLTEEERKIILAVMDRQKKKVKEEHKPQLTQWFPFSGITELVNNVLQPQQKQQNEKEPQTKLHQQFEMYKEQVKKMGEESQQQQEQKGDAPTCGICHKTKFADGCGHNCSYCQTKFCARCGGRVSLRSNKVMWVCNLCRKQQEILTKSGAWFYNSGSNTPQQPDQKVLRGLRNEEAPQEKKPKLHEQTQFQGPSGDLSVPAVEKSRSHGLTRQHSIKNGSGVKHHIASDIASDRKRSPSVSRDQNRRYDQREEREEYSQYATSDTAMPRSPSDYADRRSQHEPQFYEDSDHLSYRDSNRRSHRHSKEYIVDDEDVESRDEYERQRREEEYQSRYRSDPNLARYPVKPQPYEEQMRIHAEVSRARHERRHSDVSLANADLEDSRISMLRMDRPSRQRSISERRAAMENQRSYSMERTREAQGPSSYAQRTTNHSPPTPRRSPLPIDRPDLRRTDSLRKQHHLDPSSAVRKTKREKMETMLRNDSLSSDQSESVRPPPPKPHKSKKGGKMRQISLSSSEEELASTPEYTSCDDVEIESESVSEKGDSQKGKRKTSEQAVLSDSNTRSERQKEMMYFGGHSLEEDLEWSEPQIKDSGVDTCSSTTLNEEHSHSDKHPVTWQPSKDGDRLIGRILLNKRLKDGSVPRDSGAMLGLKVVGGKMTESGRLCAFITKVKKGSLADTVGHLRPGDEVLEWNGRLLQGATFEEVYNIILESKPEPQVELVVSRPIGDIPRIPDSTHAQLESSSSSFESQKMDRPSISVTSPMSPGMLRDVPQFLSGQLSIKLWFDKVGHQLIVTILGAKDLPSREDGRPRNPYVKIYFLPDRSDKNKRRTKTVKKTLEPKWNQTFIYSPVHRREFRERMLEITLWDQARVREEESEFLGEILIELETALLDDEPHWYKLQTHDVSSLPLPHPSPYMPRRQLHGESPTRRLQRSKRISDSEVSDYDCDDGIGVVSDYRHDGRDLQSSTLSVPEQVMSSNHCSPSGSPHRVDVIGRTRSWSPSVPPPQSRNVEQGLRGTRTMTGHYNTISRMDRHRVMDDHYSPDRDRDCEAADRQPYHRSRSTEQRPLLERTTTRSRSTERPDTNLMRSMPSLMTGRSAPPSPALSRSHPRTGSVQTSPSSTPVAGRRGRQLPQLPPKGTLDRKAGGKKLRSTVQRSTETGLAVEMRNWMTRQASRESTDGSMNSYSSEGNLIFPGVRLASDSQFSDFLDGLGPAQLVGRQTLATPAMGDIQVGMMDKKGQLEVEIIRARGLVVKPGSKTLPAPYVKVYLLDNGVCIAKKKTKVARKTLEPLYQQLLSFEESPQGKVLQIIVWGDYGRMDHKSFMGVAQILLDELELSNMVIGWFKLFPPSSLVDPTLAPLTRRASQSSLESSTGPSYSRS |
| 预测分子量 | 160 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RIMS2重组蛋白的参考文献示例(文献为虚构示例，供参考)：
1. **文献名称**: *Structural and Functional Analysis of RIMS2 in Synaptic Vesicle Exocytosis*
**作者**: Smith J, et al.
**摘要**: 本研究通过重组表达纯化RIMS2蛋白，解析其与Rab3A的相互作用结构域，揭示其在突触囊泡释放中的调控机制。实验表明RIMS2重组蛋白能增强突触前钙通道的活性。
2. **文献名称**: *RIMS2 Recombinant Protein Facilitates Insulin Secretion in Pancreatic β-Cells*
**作者**: Zhang L, et al.
**摘要**: 研究利用大肠杆菌表达系统制备RIMS2重组蛋白，发现其通过调控SNARE复合体稳定性促进β细胞胰岛素分泌，为糖尿病治疗提供潜在靶点。
3. **文献名称**: *RIMS2 Interactions with ELKS Family Proteins: A Recombinant Protein-Based Study*
**作者**: Tanaka K, et al.
**摘要**: 通过体外结合实验，作者利用重组RIMS2蛋白证明其与ELKS蛋白的直接结合，并阐明了该相互作用对突触活性区的组装至关重要。
4. **文献名称**: *Expression and Purification of RIMS2 for Neurodevelopmental Disorder Research*
**作者**: Lee S, et al.
**摘要**: 研究优化了RIMS2重组蛋白在哺乳动物细胞中的表达和纯化流程，并验证了其突变体在自闭症相关神经发育异常中的功能缺失机制。
建议通过PubMed或Google Scholar搜索关键词“RIMS2 recombinant protein”获取真实文献。
**Background of RIMS2 Recombinant Protein**
RIMS2 (Rab3-Interacting Molecule 2) is a member of the RIM protein family, which plays a pivotal role in regulating synaptic vesicle exocytosis and neurotransmitter release. Functioning as a scaffold protein at the presynaptic active zone, RIMS2 interacts with key synaptic components, including Rab3 GTPases, Munc13. and voltage-gated calcium channels. These interactions facilitate synaptic vesicle priming, calcium channel clustering, and the alignment of release machinery, ensuring efficient and precise neurotransmission. RIMS2 is particularly critical for maintaining synaptic plasticity, which underlies learning and memory processes.
Recombinant RIMS2 protein is produced using genetic engineering techniques, where the *RIMS2* gene is cloned into expression systems (e.g., bacterial, insect, or mammalian cells) to generate purified, functional protein. This allows researchers to study its structural and biochemical properties in vitro. Recombinant RIMS2 retains domains essential for its activity, such as the PDZ, C2. and zinc-finger domains, enabling investigations into its binding partners and mechanistic roles.
Interest in RIMS2 stems from its association with neurological and neurodevelopmental disorders. Mutations in the *RIMS2* gene are linked to autism spectrum disorders, intellectual disability, and schizophrenia, highlighting its importance in brain function. Recombinant RIMS2 serves as a tool to dissect these pathological mechanisms, screen therapeutic compounds, or develop diagnostic biomarkers. Additionally, it aids in exploring synapse formation and maintenance, offering insights into neurodegenerative diseases like Alzheimer’s.
Overall, RIMS2 recombinant protein bridges molecular neuroscience and translational research, advancing our understanding of synaptic biology and disorder pathogenesis.
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