| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb7 |
| Uniprot No | Q9Y5E2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-793aa |
| 氨基酸序列 | MEARVERAVQKRQVLFLCVFLGMSWAGAEPLRYFVAEETERGTFLTNLAKDLGLGVGELRARGTRIVSDQNMQILLLSSLTGDLLLNEKLDREELCGPREPCVLPFQLLLEKPFQIFRAELWVRDINDHAPVFLDREISLKILESTTPGAAFLLESAQDSDVGTNSLSNYTISPNAYFHINVHDSGEGNIYPELVLNQVLDREEIPEFSLTLTALDGGSPPRSGTALVRILVLDVNDNAPDFVRSLYKVQVPENSPVGSMVVSVSARDLDTGSNGEIAYAFSYATERILKTFQINPTSGSLHLKAQLDYEAIQTYTLTIQAKDGGGLSGKCTVVVDVTDINDNRPELLLSSLTSPIAENSPETVVAVFRIRDRDSGNNGKTVCSIQDDVPFILKPSVENFYTLVTEKPLDRERNTEYNITITVTDLGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALPIGSVSATDRDSGTNAQVIYSLLPSQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSSEALVRVLVLDANDNSPFVLYPLQNSSAPCTEPLPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKQRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLRLPEAAPDQANSLTVYLVVALASVSSLFLLSVLLFVAVRLCRRSRAAPVGRCSVPEGPFPRHLVDLSGTGTLSQSYQYEVCLTGGSGTNEFKFLKPIIPNLLPQSTGREVEENRPFQNNLGF |
| 预测分子量 | 86,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDHb7重组蛋白的3篇模拟参考文献及其摘要概括(基于公开研究领域知识，非真实文献)：
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**1. 文献名称**：*"Recombinant PCDHb7 promotes neuronal differentiation via Wnt/β-catenin signaling"*
**作者**：Chen L, et al.
**摘要**：研究通过大肠杆菌系统表达并纯化重组PCDHb7蛋白，发现其可通过激活Wnt/β-catenin通路促进神经干细胞分化为神经元，提示其在神经发育和再生医学中的潜在应用。
**2. 文献名称**：*"Structural characterization of PCDHb7 recombinant protein and its role in cell-cell adhesion"*
**作者**：Wang Y, et al.
**摘要**：利用哺乳动物HEK293细胞系表达PCDHb7重组蛋白，解析其胞外结构域晶体结构，证明其通过同源二聚化介导细胞间粘附，并调控上皮细胞屏障功能。
**3. 文献名称**：*"PCDHb7 recombinant protein suppresses tumor metastasis by inhibiting EMT in colorectal cancer"*
**作者**：Zhang R, et al.
**摘要**：研究显示，重组PCDHb7蛋白可抑制结直肠癌细胞上皮-间质转化(EMT)，降低迁移和侵袭能力，机制涉及与E-cadherin的协同作用及TGF-β信号通路抑制。
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**注**：以上内容为模拟生成，实际文献需通过PubMed、Web of Science等平台检索关键词“PCDHb7 recombinant protein”或“Protocadherin beta 7”获取。
**Background of PCDHb7 Recombinant Protein**
Protocadherin beta-7 (PCDHb7), a member of the cadherin superfamily, is a transmembrane protein involved in cell-cell adhesion and signaling. It belongs to the non-clustered protocadherin-beta subfamily, which plays critical roles in neural development, including axon guidance, synaptic connectivity, and neuronal circuit formation. PCDHb7 is characterized by its extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail that interacts with intracellular signaling molecules. Unlike classical cadherins, protocadherins exhibit combinatorial diversity in expression, contributing to the specificity of neuronal connections.
The recombinant PCDHb7 protein is engineered in vitro using molecular cloning techniques, often expressed in mammalian or bacterial systems to ensure proper folding and post-translational modifications. It serves as a valuable tool for studying cell adhesion mechanisms, neural network assembly, and disease-related pathways. Dysregulation of PCDHb7 has been implicated in neurodevelopmental disorders and cancers, with studies suggesting its role in tumor suppression or metastasis modulation depending on cellular context.
Research applications include ligand-receptor interaction assays, functional studies in neuronal models, and exploring its potential as a biomarker or therapeutic target. The recombinant form enables precise analysis of PCDHb7’s structure-function relationships, aiding drug discovery and mechanistic insights into neurological and oncological conditions.
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