| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDH18 |
| Uniprot No | Q9HCL0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1135aa |
| 氨基酸序列 | MHQMNAKMHFRFVFALLIVSFNHDVLGKNLKYRIYEEQRVGSVIARLSEDVADVLLKLPNPSTVRFRAMQRGNSPLLVVNEDNGEISIGATIDREQLCQKNLNCSIEFDVITLPTEHLQLFHIEVEVLDINDNSPQFSRSLIPIEISESAAVGTRIPLDSAFDPDVGENSLHTYSLSANDFFNIEVRTRTDGAKYAELIVVRELDRELKSSYELQLTASDMGVPQRSGSSILKISISDSNDNSPAFEQQSYIIQLLENSPVGTLLLDLNATDPDEGANGKIVYSFSSHVSPKIMETFKIDSERGHLTLFKQVDYEITKSYEIDVQAQDLGPNSIPAHCKIIIKVVDVNDNKPEININLMSPGKEEISYIFEGDPIDTFVALVRVQDKDSGLNGEIVCKLHGHGHFKLQKTYENNYLILTNATLDREKRSEYSLTVIAEDRGTPSLSTVKHFTVQINDINDNPPHFQRSRYEFVISENNSPGAYITTVTATDPDLGENGQVTYTILESFILGSSITTYVTIDPSNGAIYALRIFDHEEVSQITFVVEARDGGSPKQLVSNTTVVLTIIDENDNVPVVIGPALRNNTAEITIPKGAESGFHVTRIRAIDRDSGVNAELSCAIVAGNEENIFIIDPRSCDIHTNVSMDSVPYTEWELSVIIQDKGNPQLHTKVLLKCMIFEYAESVTSTAMTSVSQASLDVSMIIIISLGAICAVLLVIMVLFATRCNREKKDTRSYNCRVAESTYQHHPKRPSRQIHKGDITLVPTINGTLPIRSHHRSSPSSSPTLERGQMGSRQSHNSHQSLNSLVTISSNHVPENFSLELTHATPAVEQVSQLLSMLHQGQYQPRPSFRGNKYSRSYRYALQDMDKFSLKDSGRGDSEAGDSDYDLGRDSPIDRLLGEGFSDLFLTDGRIPAAMRLCTEECRVLGHSDQCWMPPLPSPSSDYRSNMFIPGEEFPTQPQQQHPHQSLEDDAQPADSGEKKKSFSTFGKDSPNDEDTGDTSTSSLLSEMSSVFQRLLPPSLDTYSECSEVDRSNSLERRKGPLPAKTVGYPQGVAAWAASTHFQNPTTNCGPPLGTHSSVQPSSKWLPAMEEIPENYEEDDFDNVLNHLNDGKHELMDASELVAEINKLLQDVRQS |
| 预测分子量 | 126 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PCDH18重组蛋白的参考文献示例(注：以下内容为模拟示例，实际文献可能需要根据具体数据库检索)：
1. **文献名称**：*"Recombinant human PCDH18 protein promotes neuronal differentiation in vitro"*
**作者**：Chen L, et al.
**摘要**：本研究通过大肠杆菌系统表达了重组人源PCDH18蛋白，并验证其促进神经干细胞向神经元分化的功能，表明PCDH18在神经发育中可能起关键作用。
2. **文献名称**：*"Structural and functional analysis of PCDH18 recombinant protein in cell-cell adhesion"*
**作者**：Kim S, et al.
**摘要**：利用昆虫细胞表达系统制备PCDH18重组蛋白，通过体外实验证明其介导钙离子依赖性细胞粘附，并解析了其胞外结构域的晶体结构。
3. **文献名称**：*"PCDH18 recombinant protein suppresses melanoma metastasis via Wnt signaling inhibition"*
**作者**：Wang Y, et al.
**摘要**：研究发现重组PCDH18蛋白通过抑制Wnt/β-catenin通路，降低黑色素瘤细胞的迁移和侵袭能力，提示其作为潜在治疗靶点。
4. **文献名称**：*"Development of a high-yield PCDH18 recombinant protein expression system for antibody production"*
**作者**：Garcia R, et al.
**摘要**：优化了哺乳动物细胞中PCDH18重组蛋白的表达和纯化工艺，用于生成特异性抗体，为后续功能研究和诊断应用奠定基础。
**注意**：以上为模拟文献，实际引用请通过PubMed、Web of Science或Google Scholar检索真实发表的研究。
Protocadherin-18 (PCDH18), a member of the protocadherin family, is a calcium-dependent cell adhesion protein implicated in tissue development and cellular signaling. As a transmembrane protein, it features extracellular cadherin repeat domains that mediate homophilic or heterophilic interactions, a single-pass transmembrane region, and a cytoplasmic tail that may recruit intracellular signaling partners. The protocadherin family is known for its role in neural development, including axon guidance, synaptic plasticity, and neuronal self-avoidance, but PCDH18 has also been linked to non-neural processes such as vascular remodeling and cancer progression.
Recombinant PCDH18 protein is engineered for in vitro studies to dissect its molecular functions, interactions, and therapeutic potential. Produced via expression systems like mammalian cells or insect cells, the recombinant form retains post-translational modifications critical for its biological activity. Researchers use it to investigate PCDH18’s role in cell-cell adhesion, signaling pathways (e.g., Wnt/β-catenin or MAPK), and its dysregulation in diseases. For example, PCDH18 is reported to act as a tumor suppressor in some cancers by inhibiting cell proliferation or metastasis, while its overexpression in other contexts may promote angiogenesis or tissue fibrosis.
Additionally, recombinant PCDH18 serves as an antigen for antibody development or diagnostic assays. Its study contributes to understanding developmental disorders, neurodegenerative conditions, and cancer biology, offering potential targets for therapies aimed at modulating cell adhesion or signaling pathways. Current research focuses on clarifying its tissue-specific roles and interaction networks, which remain partially characterized.
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