| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAPST1 |
| Uniprot No | Q8TB61 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-432aa |
| 氨基酸序列 | MDARWWAVVVLAAFPSLGAGGETPEAPPESWTQLWFFRFVVNAAGYASFMVPGYLLVQYFRRKNYLETGRGLCFPLVKACVFGNEPKASDEVPLAPRTEAAETTPMWQALKLLFCATGLQVSYLTWGVLQERVMTRSYGATATSPGERFTDSQFLVLMNRVLALIVAGLSCVLCKQPRHGAPMYRYSFASLSNVLSSWCQYEALKFVSFPTQVLAKASKVIPVMLMGKLVSRRSYEHWEYLTATLISIGVSMFLLSSGPEPRSSPATTLSGLILLAGYIAFDSFTSNWQDALFAYKMSSVQMMFGVNFFSCLFTVGSLLEQGALLEGTRFMGRHSEFAAHALLLSICSACGQLFIFYTIGQFGAAVFTIIMTLRQAFAILLSCLLYGHTVTVVGGLGVAVVFAALLLRVYARGRLKQRGKKAVPVESPVQKV |
| 预测分子量 | 47,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PAPST1重组蛋白的模拟参考文献示例(仅供参考，具体文献需根据实际研究查询)：
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1. **文献名称**: *Functional Characterization of PAPST1 in Lysosomal Sulfate Transport*
**作者**: Girard, M., et al.
**摘要**: 本研究阐明了PAPST1作为溶酶体膜硫磺酸转运蛋白的功能，通过重组蛋白表达实验证实其介导磷酸腺苷磷硫酸盐(PAPS)的跨膜运输，并证明其缺陷会导致硫酸酯酶活性降低，影响细胞代谢。
2. **文献名称**: *Recombinant PAPST1 Protein Purification and Structural Insights*
**作者**: Lee, S.J., & Kim, H.
**摘要**: 报道了重组人源PAPST1蛋白在大肠杆菌中的高效表达与纯化方法，结合结晶学分析揭示了其底物结合域的关键氨基酸残基，为靶向药物设计提供结构基础。
3. **文献名称**: *PAPST1 Deficiency and Its Link to Sulfation Disorders*
**作者**: Roberts, A.L., et al.
**摘要**: 综述了PAPST1基因突变与遗传性硫磺酸转运障碍疾病的关联，通过体外重组蛋白功能实验证明突变体导致PAPS转运能力丧失，提示其作为潜在治疗靶点。
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注：以上为基于领域知识的模拟文献，实际引用时请通过学术数据库(如PubMed、Web of Science)检索最新实证研究。
**Background of PAPST1 Recombinant Protein**
PAPST1 (3'-phosphoadenosine 5'-phosphosulfate transporter 1), also known as SLC35B2. is a member of the solute carrier family 35 (SLC35), which facilitates the transport of nucleotide sugars and other nucleotide-linked molecules across cellular membranes. Specifically, PAPST1 is recognized as a key transporter of 3'-phosphoadenosine 5'-phosphosulfate (PAPS), a universal sulfonate donor required for sulfation reactions in the Golgi apparatus. Sulfation is a critical post-translational modification involved in the biosynthesis of glycoproteins, proteoglycans, and other macromolecules, influencing cell signaling, extracellular matrix organization, and metabolic regulation.
The recombinant PAPST1 protein is engineered to study its structure, function, and interactions in vitro. Produced using recombinant DNA technology, it is typically expressed in mammalian or insect cell systems to ensure proper post-translational modifications and membrane localization. Researchers utilize this protein to investigate PAPS transport mechanisms, its role in sulfation pathways, and its association with diseases linked to sulfation defects, such as skeletal dysplasia, immune dysfunction, and cancer.
PAPST1’s dysfunction has been implicated in impaired glycosaminoglycan sulfation, affecting connective tissue integrity and cellular communication. Its recombinant form enables high-throughput screening for drug candidates targeting sulfation-related disorders and provides a tool for elucidating Golgi transport dynamics. Studies also explore its potential as a biomarker or therapeutic target in pathologies where sulfation is dysregulated. Overall, the PAPST1 recombinant protein serves as a vital resource for advancing molecular and therapeutic research in cell biology and disease mechanisms.
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