| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHgA9 |
| Uniprot No | Q9Y5G4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-932aa |
| 氨基酸序列 | MAAPTKCQLRGRLVLLCSLLGMLWEARASQIRYSVPEETEKGYIVGNISKDLALEPRELAERRVRIVSRGRTQLFSLNPRSGTLVTAGRIDREELCAQSPRCLVNFKVLVEDRVKLYGIEIEVTDINDSAPKFQAESLEVKINEIAVPGARYPLPEAIDPDVGVNSLQSYQLSPNHHFSLNVQTGDNGAINPELVLERALDREEATAHHLVLTASDGGEPRRSSTVRIHVTVLDTNDNAPVFAQRIYRVKVLENVPPGTWLLTATASDLDEGINGKVAYKFWKINEKQSLLFQLNENTGEISTAKSLDYEECSFYEMEIQAEDGGGLKGWTKVLISVEDVNDNRPEVTITSLFSPVREDAPQGTVILLFNAHDRDSGKNGQVVCSIQENLSFTLENSEEDYYRLLTAQILDREKASEYNITVTATDRGTPPLSTEIHITLQVTDINDNPPAFSQASYSVYLPENNARGTSIFSVIAYDPDSNENSRVIYSLAEDTIQGSPLSTYVSINSDTGVLYALCSFDYEQFRDLQMQVTASDSGSPPLSSNVSLRLFVLDQNDNAPEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYRLFKASEPGLFSVGLHTGEVRTARALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAIADSIPDILADLGSLQIPADLEASDLTLYLVVAVAVVSCVFLTFVITLLALRLRHWHSSHLLRATSDGLAGVPTSHFVGVDGVRAFLQTYSQEFSLTADSRKSHLIFPQPNYADTLISQQSCEKNEPLCVSVDSKFPIEDTPLVPQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于 **PCDHGA9(原钙粘蛋白γA9)重组蛋白** 的参考文献及其简要摘要：
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1. **文献名称**: *"Recombinant Protocadherin-γA9 regulates synaptic plasticity in hippocampal neurons"*
**作者**: Smith J, et al.
**摘要**: 研究利用HEK293细胞表达重组PCDHGA9蛋白，验证其通过钙依赖性机制促进神经突触形成，并调控神经元间的特异性粘附，揭示其在神经回路发育中的潜在作用。
2. **文献名称**: *"Structural and functional analysis of PCDHGA9 extracellular domain in cancer cell migration"*
**作者**: Zhang L, et al.
**摘要**: 通过大肠杆菌系统表达重组PCDHGA9胞外结构域，结合晶体结构解析和功能实验，发现其抑制肿瘤细胞迁移的机制可能与Wnt/β-catenin信号通路拮抗相关。
3. **文献名称**: *"PCDHGA9 as a cell-cell adhesion molecule: Role of recombinant protein in epithelial integrity"*
**作者**: Chen X, et al.
**摘要**: 在哺乳动物细胞中重组表达PCDHGA9.证明其通过同源相互作用增强上皮细胞单层屏障功能，并依赖钙离子介导的细胞间黏附，提示其在组织稳态中的重要性。
4. **文献名称**: *"High-throughput screening of PCDHGA9-binding partners using recombinant extracellular domain fusion proteins"*
**作者**: Kim S, et al.
**摘要**: 构建带有标签的PCDHGA9重组胞外区蛋白，通过蛋白质芯片筛选出多种潜在配体(如神经连接蛋白)，为解析原钙粘蛋白家族互作网络提供工具。
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**注**：以上文献为示例，实际引用需根据具体研究内容检索真实发表的论文(可通过PubMed/Google Scholar搜索关键词：PCDHGA9 recombinant, Protocadherin gamma A9 expression)。
The protocadherin gamma subfamily A9 (PCDHγA9), a member of the clustered protocadherin (PcDH) superfamily, is a calcium-dependent cell adhesion molecule predominantly expressed in the nervous system. It plays a critical role in neural circuit assembly, synaptic specificity, and neuronal self-avoidance through homophilic interactions. The PCDHγ cluster consists of 22 variable exons (A1–A12. B1–B7. and C3–C5), with PCDHγA9 being one of the "A-type" isoforms characterized by a conserved cytoplasmic domain involved in intracellular signaling. Dysregulation of PCDHγ genes has been linked to neurodevelopmental disorders, neuroinflammation, and cancer metastasis.
Recombinant PCDHγA9 protein is typically produced via heterologous expression systems (e.g., mammalian or insect cells) to preserve post-translational modifications and structural integrity. Its extracellular domain contains six cadherin-like repeats that mediate selective cell-cell recognition, while the intracellular domain interacts with cytosolic proteins like TAF1/Set to regulate dendritic arborization. Research applications include studying neural network formation, synapse dynamics, and tumor suppression mechanisms. In oncology, altered PCDHγA9 expression correlates with epithelial-mesenchymal transition (EMT) in carcinomas, making it a potential biomarker or therapeutic target. Recent studies also explore its role in modulating microglial activity and neuroimmune responses. Standardized recombinant PCDHγA9 enables functional assays, antibody development, and structural studies to decipher its role in health and disease.
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