| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R3D |
| Uniprot No | O95685 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-299aa |
| 氨基酸序列 | MSRGPSSAVL PSALGSRKLG PRSLSCLSDL DGGVALEPRA CRPPGSPGRA PPPTPAPSGC DPRLRPIILR RARSLPSSPE RRQKAAGAPG AACRPGCSQK LRVRFADALG LELAQVKVFN AGDDPSVPLH VLSRLAINSD LCCSSQDLEF TLHCLVPDFP PPVEAADFGE RLQRQLVCLE RVTCSDLGIS GTVRVCNVAF EKQVAVRYTF SGWRSTHEAV ARWRGPAGPE GTEDVFTFGF PVPPFLLELG SRVHFAVRYQ VAGAEYWDNN DHRDYSLTCR NHALHMPRGE CEESWIHFI |
| 预测分子量 | 32,5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R3D重组蛋白的示例性参考文献(注：部分内容可能为模拟概括，仅供参考)：
1. **文献名称**: "Recombinant PPP1R3D Expression Enhances Glycogen Synthesis via Protein Phosphatase 1 Interaction"
**作者**: Smith J, et al.
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了重组PPP1R3D蛋白，证实其通过与蛋白磷酸酶1(PP1)结合调控肝细胞糖原合成酶的活性，揭示了其在糖代谢中的潜在作用机制。
2. **文献名称**: "Structural and Functional Analysis of PPP1R3D in Insulin Signaling Pathways"
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了重组PPP1R3D蛋白的结构，发现其C端结构域对PP1的靶向结合至关重要;体外实验表明PPP1R3D缺失会削弱胰岛素诱导的糖原积累。
3. **文献名称**: "PPP1R3D Overexpression Promotes Tumor Glycolysis in Colorectal Cancer Models"
**作者**: Zhang R, et al.
**摘要**: 构建了重组PPP1R3D过表达细胞模型，发现其通过激活糖酵解关键酶促进结直肠癌细胞增殖，提示PPP1R3D可能作为癌症代谢治疗的潜在靶点。
4. **文献名称**: "Development of a PPP1R3D Knockout Mouse Using CRISPR/Cas9 Technology"
**作者**: Garcia M, et al.
**摘要**: 利用重组蛋白互作筛选技术，发现PPP1R3D缺失小鼠表现出肝脏糖原储存异常，进一步验证了其在体内能量稳态调控中的功能。
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**注意**：以上内容为示例性概括，实际文献需通过PubMed、Web of Science等学术平台检索确认。若需真实文献，建议使用关键词“PPP1R3D recombinant protein”或“PPP1R3D PP1 regulatory subunit”进行精准查询。
**Background of PPP1R3D Recombinant Protein**
PPP1R3D (Protein Phosphatase 1 Regulatory Subunit 3D) is a member of the PPP1R3 family, which regulates the activity of protein phosphatase 1 (PP1), a serine/threonine phosphatase involved in diverse cellular processes. PPP1R3D acts as a targeting subunit that directs PP1 to specific substrates or cellular compartments, particularly in glycogen metabolism. It facilitates glycogen synthesis by promoting the dephosphorylation and activation of glycogen synthase (GS), a rate-limiting enzyme in glycogen production. Structurally, PPP1R3D contains conserved domains for PP1 binding and glycogen targeting, enabling its role in metabolic regulation.
The recombinant PPP1R3D protein is engineered for *in vitro* studies to dissect its molecular interactions, regulatory mechanisms, and physiological relevance. Produced via prokaryotic (e.g., *E. coli*) or eukaryotic expression systems, the recombinant protein retains functional domains necessary for binding PP1 and modulating glycogen-related pathways. Its applications include elucidating PPP1R3D’s role in glucose homeostasis, insulin signaling, and cellular energy balance. Dysregulation of PPP1R3D has been linked to metabolic disorders, such as type 2 diabetes, and certain cancers, where aberrant glycogen accumulation or signaling may drive pathogenesis.
Research using recombinant PPP1R3D also explores its potential as a therapeutic target. For instance, inhibitors or activators of PPP1R3D-PP1 interactions could restore glycogen metabolism in metabolic diseases. Additionally, structural studies leveraging recombinant proteins help identify mutation-associated dysfunctions or design small-molecule modulators. Overall, PPP1R3D recombinant tools are vital for advancing understanding of glycogen regulation and its implications in health and disease.
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