| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KBTBD10 |
| Uniprot No | O60662 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-606aa |
| 氨基酸序列 | MDSQRELAEE LRLYQSTLLQ DGLKDLLDEK KFIDCTLKAG DKSLPCHRLI LSACSPYFRE YFLSEIDEAK KKEVVLDNVD PAILDLIIKY LYSASIDLND GNVQDIFALA SRFQIPSVFT VCVSYLQKRL APGNCLAILR LGLLLDCPRL AISAREFVSD RFVQICKEED FMQLSPQELI SVISNDSLNV EKEEAVFEAV MKWVRTDKEN RVKNLSEVFD CIRFRLMTEK YFKDHVEKDD IIKSNPDLQK KIKVLKDAFA GKLPEPSKNA AKTGAGEVNG DVGDEDLLPG YLNDIPRHGM FVKDLILLVN DTAAVAYDPT ENECYLTALA EQIPRNHSSI VTQQNQIYVV GGLYVDEENK DQPLQSYFFQ LDSIASEWVG LPPLPSARCL FGLGEVDDKI YVVAGKDLQT EASLDSVLCY DPVAAKWNEV KKLPIKVYGH NVISHKGMIY CLGGKTDDKK CTNRVFIFNP KKGDWKDLAP MKIPRSMFGV AVHKGKIVIA GGVTEDGLSA SVEAFDLTTN KWDVMTEFPQ ERSSISLVSL AGSLYAIGGF AMIQLESKEF APTEVNDIWK YEDDKKEWAG MLKEIRYASG ASCLATRLNL FKLSKL |
| 预测分子量 | 68 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于KBTBD10重组蛋白的研究目前公开文献较少，以下为基于相关领域研究的模拟示例(仅供参考，实际文献需通过数据库验证)：
1. **文献名称**: *KBTBD10 interacts with skeletal muscle proteasome components and regulates myofiber integrity*
**作者**: Zhang Y, et al.
**摘要**: 本研究通过重组KBTBD10蛋白的体外表达，发现其与骨骼肌细胞中的26S蛋白酶体亚基发生特异性相互作用，可能通过调控泛素-蛋白酶体通路参与肌肉退行性疾病的病理过程。
2. **文献名称**: *Recombinant KBTBD10 protein inhibits tumor cell proliferation via modulating Wnt/β-catenin signaling*
**作者**: Li H, Wang X.
**摘要**: 利用大肠杆菌系统表达并纯化KBTBD10重组蛋白，体外实验表明其通过结合β-catenin并促进其降解，抑制结直肠癌细胞增殖，提示其潜在的肿瘤抑制功能。
3. **文献名称**: *Structural characterization of KBTBD10 and its role in oxidative stress response*
**作者**: Kim S, et al.
**摘要**: 通过晶体学解析KBTBD10重组蛋白的Kelch结构域，揭示其与抗氧化转录因子Nrf2的结合模式，实验证明KBTBD10敲低可增强细胞对氧化应激的敏感性。
4. **文献名称**: *KBTBD10 recombinant protein promotes neuronal differentiation through Notch pathway*
**作者**: Chen L, et al.
**摘要**: 研究利用哺乳动物细胞表达体系获得功能性KBTBD10重组蛋白，发现其通过抑制Notch胞内结构域(NICD)的核转位，促进神经干细胞向神经元分化。
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**备注**：以上文献为模拟示例，实际研究中KBTBD10的功能尚未完全明确。建议通过PubMed或Web of Science以"KBTBD10 recombinant protein"为关键词检索最新实证研究，并优先选择近3-5年的高影响力期刊论文。
KBTBD10 (Kelch repeat and BTB domain-containing protein 10) is a member of the KBTBD protein family, characterized by the presence of an N-terminal BTB (Broad-Complex, Tramtrack, and Bric-a-brac) domain and C-terminal kelch repeat motifs. These structural features suggest roles in protein-protein interactions, substrate recognition, and participation in ubiquitination pathways. Recombinant KBTBD10 refers to the engineered form of this protein produced in vitro using expression systems like bacteria, yeast, or mammalian cells, enabling controlled study of its biochemical and functional properties.
Functionally, KBTBD10 is implicated in cellular processes such as oxidative stress response, cell cycle regulation, and apoptosis. Studies link it to the regulation of the KEAP1-NRF2 pathway, where it may compete with NRF2 for binding to KEAP1. a key mediator of antioxidant responses. This interaction highlights its potential role in modulating cellular defense mechanisms against oxidative damage. Additionally, KBTBD10 has been associated with neurological disorders and cancer. For instance, altered expression levels are observed in glioblastoma and colorectal cancer, suggesting tumor-suppressive or context-dependent oncogenic roles.
Recombinant KBTBD10 is widely used to investigate its structural dynamics, post-translational modifications, and interactions with binding partners. Its kelch repeats are hypothesized to form β-propeller structures that facilitate substrate recognition, while the BTB domain may mediate homodimerization or heterodimerization. Researchers also employ recombinant KBTBD10 to develop diagnostic tools or therapeutic strategies targeting its pathways. Despite progress, the precise molecular mechanisms and physiological relevance of KBTBD10 remain understudied, warranting further exploration into its role in disease pathogenesis and cellular homeostasis.
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