| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | LRP12 |
| Uniprot No | Q9Y561 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 28-488aa |
| 氨基酸序列 | EHSENVHISGVSTACGETPEQIRAPSGIITSPGWPSEYPAKINCSWFIRA NPGEIITISFQDFDIQGSRRCNLDWLTIETYKNIESYRACGSTIPPPYIS SQDHIWIRFHSDDNISRKGFRLAYFSGKSEEPNCACDQFRCGNGKCIPEA WKCNNMDECGDSSDEEICAKEANPPTAAAFQPCAYNQFQCLSRFTKVYTC LPESLKCDGNIDCLDLGDEIDCDVPTCGQWLKYFYGTFNSPNYPDFYPPG SNCTWLIDTGDHRKVILRFTDFKLDGTGYGDYVKIYDGLEENPHKLLRVL TAFDSHAPLTVVSSSGQIRVHFCADKVNAARGFNATYQVDGFCLPWEIPC GGNWGCYTEQ QRCDGYWHCPNGRDETNCTMCQKEEFPCSRNGVCYPRSDRCNYQNHCPNG SDEKNCFFCQ PGNFHCKNNRCVFESWVCDSQDDCGDGSDEENCPVIVDHHHHHH |
| 预测分子量 | 53 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于LRP12重组蛋白的3篇参考文献的虚构示例(基于研究领域常见方向)：
1. **标题**: *"Recombinant Expression and Functional Characterization of LRP12 in Wnt/β-Catenin Signaling"*
**作者**: Zhang, Y. et al.
**摘要**: 本研究利用昆虫细胞系统表达并纯化了人源LRP12重组蛋白，通过体外结合实验证实其与Wnt配体的直接互作。进一步功能实验表明，LRP12通过抑制β-catenin核转位负调控Wnt信号通路，提示其在胚胎发育中的潜在作用。
2. **标题**: *"LRP12 Recombinant Protein Attenuates TGF-β1-Induced Fibrosis in Pulmonary Cells"*
**作者**: Chen, L. et al.
**摘要**: 作者通过哺乳动物表达系统获得高纯度LRP12胞外域重组蛋白，并发现其可竞争性结合TGF-β1.抑制成纤维细胞活化标志物α-SMA的表达。该研究为肺纤维化治疗提供了新的分子靶点。
3. **标题**: *"Cryo-EM Structure of LRP12 Reveals a Unique Dimerization Interface Regulating Endocytosis"*
**作者**: Müller, R. et al.
**摘要**: 本研究解析了LRP12胞外区3.2Å冷冻电镜结构，发现其通过新型二聚化界面形成同源二聚体。功能实验证明该结构域对其内吞功能至关重要，为理解LRP家族蛋白的膜运输机制提供结构基础。
注：以上为模拟文献，实际文献需通过PubMed/Google Scholar等平台以"LRP12 recombinant protein"等关键词检索。若需真实文献线索，可重点关注LRP家族蛋白共性研究或特定疾病(如癌症、纤维化)相关研究。
**Background of LRP12 Recombinant Protein**
The Low-Density Lipoprotein Receptor-Related Protein 12 (LRP12), also known as LRP10. belongs to the LDL receptor family, a group of transmembrane proteins involved in endocytosis, cell signaling, and lipid metabolism. LRP12 is characterized by its modular structure, including ligand-binding LDLR class A domains, epidermal growth factor (EGF)-like repeats, and a cytoplasmic tail with signaling motifs. It is expressed in various tissues, including the brain, liver, and reproductive organs, and plays roles in cellular processes such as receptor internalization, Wnt signaling modulation, and intracellular trafficking.
Interest in LRP12 has grown due to its association with human diseases. Studies link LRP12 mutations to neurological disorders like hereditary spastic paraplegia and amyotrophic lateral sclerosis (ALS), suggesting its role in neuronal maintenance. Additionally, LRP12 dysregulation has been implicated in cancers, including hepatocellular carcinoma and glioblastoma, where it may influence tumor progression via pathways like TGF-β or Notch signaling.
Recombinant LRP12 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional protein for research. This tool enables mechanistic studies, such as mapping ligand interactions, analyzing post-translational modifications, or screening therapeutic compounds. Its applications extend to developing diagnostic assays and understanding disease mechanisms, offering potential for targeted therapies.
Overall, LRP12 recombinant protein serves as a critical resource for unraveling the biological significance of LRP12 in health and disease, bridging structural insights with functional and clinical relevance.
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