| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP2R3a |
| Uniprot No | Q06190 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1150aa |
| 氨基酸序列 | MAATYRLVVSTVNHYSSVVIDRRFEQAIHYCTGTCHTFTHGIDCIVVHHSVCADLLHIPVSQFKDADLNSMFLPHENGLSSAEGDYPQQAFTGIPRVKRGSTFQNTYNLKDIAGEAISFASGKIKEFSFEKLKNSNHAAYRKGRKVKSDSFNRRSVDLDLLCGHYNNDGNAPSFGLLRSSSVEEKPLSHRNSLDTNLTSMFLQNFSEEDLVTQILEKHKIDNFSSGTDIKMCLDILLKCSEDLKKCTDIIKQCIKKKSGSSISEGSGNDTISSSETVYMNVMTRLASYLKKLPFEFMQSGNNEALDLTELISNMPSLQLTPFSPVFGTEQPPKYEDVVQLSASDSGRFQTIELQNDKPNSRKMDTVQSIPNNSTNSLYNLEVNDPRTLKAVQVQSQSLTMNPLENVSSDDLMETLYIEEESDGKKALDKGQKTENGPSHELLKVNEHRAEFPEHATHLKKCPTPMQNEIGKIFEKSFVNLPKEDCKSKVSKFEEGDQRDFTNSSSQEEIDKLLMDLESFSQKMETSLREPLAKGKNSNFLNSHSQLTGQTLVDLEPKSKVSSPIEKVSPSCLTRIIETNGHKIEEEDRALLLRILESIEDFAQELVECKSSRGSLSQEKEMMQILQETLTTSSQANLSVCRSPVGDKAKDTTSAVLIQQTPEVIKIQNKPEKKPGTPLPPPATSPSSPRPLSPVPHVNNVVNAPLSINIPRFYFPEGLPDTCSNHEQTLSRIETAFMDIEEQKADIYEMGKIAKVCGCPLYWKAPMFRAAGGEKTGFVTAQSFIAMWRKLLNNHHDDASKFICLLAKPNCSSLEQEDFIPLLQDVVDTHPGLTFLKDAPEFHSRYITTVIQRIFYTVNRSWSGKITSTEIRKSNFLQTLALLEEEEDINQITDYFSYEHFYVIYCKFWELDTDHDLYISQADLSRYNDQASSSRIIERIFSGAVTRGKTIQKEGRMSYADFVWFLISEEDKRNPTSIEYWFRCMDVDGDGVLSMYELEYFYEEQCERMEAMGIEPLPFHDLLCQMLDLVKPAVDGKITLRDLKRCRMAHIFYDTFFNLEKYLDHEQRDPFAVQKDVENDGPEPSDWDRFAAEEYETLVAEESAQAQFQEGFEDYETDEPASPSEFGNKSNKILSASLPEKCGKLQSVDEE |
| 预测分子量 | 130 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP2R3a重组蛋白的3篇参考文献及其摘要内容：
---
1. **文献名称**：*Structural Insights into the Regulatory Subunit PPP2R3A of Protein Phosphatase 2A*
**作者**：Stanevich, V. et al.
**摘要**：该研究解析了PPP2R3A重组蛋白的晶体结构，揭示了其作为PP2A全酶复合物调节亚基的作用机制，阐明了其通过特定结构域与其他亚基及底物结合的分子基础。
---
2. **文献名称**：*PPP2R3A Regulates Cell Cycle Progression Through PP2A-Mediated Dephosphorylation*
**作者**：Kremmer, E. et al.
**摘要**：文章发现PPP2R3A通过招募PP2A催化亚基调控细胞周期关键蛋白(如CDK1)的去磷酸化，影响G2/M期转换，强调了其在细胞周期检查点中的功能。
---
3. **文献名称**：*PPP2R3A Dysfunction in Cancer: Implications for Tumor Suppression*
**作者**：Janssens, V. & Goris, J.
**摘要**：综述了PPP2R3A作为肿瘤抑制因子的作用，指出其在多种癌症中表达异常，并通过PP2A依赖的信号通路(如MAPK和Wnt)抑制肿瘤生长。
---
以上研究涵盖了PPP2R3a的结构、功能及疾病关联，可供进一步参考。
**Background of PPP2R3A Recombinant Protein**
PPP2R3A (Protein Phosphatase 2 Regulatory Subunit B'' Alpha) is a regulatory component of the serine/threonine protein phosphatase 2A (PP2A) complex, a critical enzyme involved in diverse cellular processes, including cell cycle regulation, signal transduction, and apoptosis. As part of the B'' family (also termed the B56 subfamily), PPP2R3A functions as a scaffolding subunit that directs PP2A’s substrate specificity and subcellular localization by forming distinct holoenzyme complexes with catalytic (C) and structural (A) subunits.
Recombinant PPP2R3A is produced through molecular cloning and expression systems (e.g., *E. coli* or mammalian cells), enabling controlled studies of its structure-function relationships. Its recombinant form typically retains conserved domains, such as the heat-repeat motifs essential for binding PP2A core enzymes and regulatory partners. Research highlights its role in modulating PP2A activity toward specific targets, including proteins involved in Wnt/β-catenin signaling, DNA damage response, and cytoskeletal dynamics.
Dysregulation of PPP2R3A has been implicated in diseases, including cancers and neurodegenerative disorders, underscoring its therapeutic relevance. For instance, altered expression or mutations in PPP2R3A may disrupt PP2A-mediated tumor suppression or tau dephosphorylation in Alzheimer’s disease. Recombinant PPP2R3A serves as a vital tool for dissecting PP2A’s regulatory mechanisms, screening potential drugs targeting PP2A pathways, and exploring its interactions with viral proteins or oncogenic kinases.
Overall, PPP2R3A recombinant protein bridges structural, biochemical, and biomedical research, offering insights into PP2A’s multifaceted roles in health and disease.
×
