| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP1R3 |
| Uniprot No | Q16821 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1122aa |
| 氨基酸序列 | MEPSEVPSQISKDNFLEVPNLSDSLCEDEEVTFQPGFSPQPSRRGSDSSEDIYLDTPSSGTRRVSFADSFGFNLVSVKEFDCWELPSASTTFDLGTDIFHTEEYVLAPLFDLPSSKEDLMQQLQIQKAILESTESLLGSTSIKGIIRVLNVSFEKLVYVRMSLDDWQTHYDILAEYVPNSCDGETDQFSFKIVLVPPYQKDGSKVEFCIRYETSVGTFWSNNNGTNYTFICQKKEQEPEPVKPWKEVPNRQIKGCLKVKSSKEESSVTSEENNFENPKNTDTYIPTIICSHEDKEDLEASNRNVKDVNREHDEHNEKELELMINQHLIRTRSTASRDERNTFSTDPVNFPNKAEGLEKKQIHGEICTDLFQRSLSPSSSAESSVKGDFYCNEKYSSGDDCTHQPSEETTSNMGEIKPSLGDTSSDELVQLHTGSKEVLDDNANPAHGNGTVQIPCPSSDQLMAGNLNKKHEGGAKNIEVKDLGCLRRDFHSDTSACLKESTEEGSSKEDYYGNGKDDEEQRIYLGVNEKQRKNFQTILHDQERKMGNPKISVAGIGASNRDLATLLSEHTAIPTRAITADVSHSPRTNLSWEEAVLTPEHHHLTSEGSALGGITGQVCSSRTGNVLRNDYLFQVEEKSGGINSEDQDNSPQHKQSWNVLESQGKSRENKTNITEHIKGQTDCEDVWGKRDNTRSLKATTEELFTCQETVCCELSSLADHGITEKAEAGTAYIIKTTSESTPESMSAREKAIIAKLPQETARSDRPIEVKETAFDPHEGRNDDSHYTLCQRDTVGVIYDNDFEKESRLGICNVRVDEMEKEETMSMYNPRKTHDREKCGTGNITSVEESSWVITEYQKATSKLDLQLGMLPTDKTVFSENRDLRQVQELSKKTDSDAIVHSAFNSDTNRAPQNSSPFSKHHTEISVSTNEQAIAVENAVTTMASQPISTKSENICNSTREIQGIEKHPYPESKPEEVSRSSGIVTSGSRKERCIGQIFQTEEYSVEKSLGPMILINKPLENMEEARHENEGLVSSGQSLYTSGEKESDSSASTSLPVEESQAQGNESLFSKYTNSKIPYFLLFLIFLITVYHYDLMIGLTFYVLSLSWLSWEEGRQKESVKKK |
| 预测分子量 | 125 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP1R3重组蛋白的参考文献示例(注：以下为模拟文献，实际引用需查询真实数据库)：
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1. **标题**: *"Recombinant PPP1R3B Enhances Glycogen Synthesis by Regulating PP1 Activity in Hepatocytes"*
**作者**: Smith, J., Brown, K., & Cohen, P.
**摘要**: 研究通过表达重组PPP1R3B蛋白，发现其与蛋白磷酸酶1(PP1)结合，激活糖原合成酶，显著促进肝细胞糖原积累，为糖尿病治疗提供潜在靶点。
2. **标题**: *"Structural and Functional Characterization of Human PPP1R3A Recombinant Protein"*
**作者**: Lee, H., Zhang, Y., & Li, X.
**摘要**: 利用大肠杆菌系统表达并纯化PPP1R3A重组蛋白，通过冷冻电镜解析其与PP1的复合物结构，揭示其调控糖原代谢的分子机制。
3. **标题**: *"PPP1R3C Recombinant Protein Ameliorates Insulin Resistance in Skeletal Muscle Cells"*
**作者**: Garcia-Roves, P., & Printen, J.
**摘要**: 在骨骼肌细胞中过表达重组PPP1R3C蛋白，发现其通过增强胰岛素信号通路改善糖原合成，提示其在代谢综合征中的作用。
4. **标题**: *"Mutations in PPP1R3G Recombinant Protein Disrupt Glycogen Binding in Rare Metabolic Disorders"*
**作者**: Suzuki, Y., Johnson, L., & Armstrong, C.
**摘要**: 分析罕见遗传病中PPP1R3G突变体的重组蛋白功能，发现其糖原结合能力受损，导致糖原储存异常，为疾病机制提供新见解。
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**建议**：上述文献为示例，实际研究中请通过 **PubMed**、**Google Scholar** 或 **Web of Science** 检索真实文献，关键词可尝试“PPP1R3 recombinant”“PPP1R3 glycogen”“PPP1R3 phosphatase regulation”等。
**Background of PPP1R3 Recombinant Protein**
PPP1R3 (Protein Phosphatase 1 Regulatory Subunit 3) belongs to a family of regulatory proteins that modulate the activity of protein phosphatase 1 (PP1), a serine/threonine phosphatase critical for cellular processes like glycogen metabolism, cell cycle progression, and signal transduction. Specifically, PPP1R3 proteins act as targeting subunits, directing PP1 to specific substrates or subcellular locations. The PPP1R3 family includes several isoforms (e.g., PPP1R3A, B, C, D), with PPP1R3C (also known as PTG) being extensively studied for its role in glycogen regulation. It facilitates the dephosphorylation of glycogen synthase (GS) and glycogen phosphorylase (GP), promoting glycogen synthesis and storage in tissues like liver and muscle.
Recombinant PPP1R3 proteins are engineered using expression systems (e.g., *E. coli* or mammalian cells) to produce purified, functional proteins for research. These proteins retain the ability to bind PP1 and glycogen-related enzymes, enabling studies on glycogen metabolism dysregulation linked to diseases such as type 2 diabetes, insulin resistance, and glycogen storage disorders. For example, overexpression of PPP1R3C in hepatocytes enhances glycogen accumulation, while its deficiency correlates with impaired glucose homeostasis.
Research on PPP1R3 recombinant proteins also explores their interaction with insulin signaling pathways and allosteric regulators (e.g., glucose-6-phosphate). Such work provides insights into metabolic disorders and potential therapeutic strategies targeting glycogen synthesis. Additionally, structural studies using recombinant PPP1R3 help map binding domains critical for PP1 interaction and substrate specificity. Overall, PPP1R3 recombinant proteins serve as vital tools to dissect the molecular mechanisms of energy metabolism and develop interventions for metabolic diseases.
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