| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | AMOTL2 |
| Uniprot No | Q9Y2J4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 314-779aa |
| 氨基酸序列 | MEAVLRENARLQRDNERLQRELESSAEKAGRIEKLESEIQRLSEAHESLT RASSKREALEKTMRNKMDSEMRRLQDFNRDLRERLESANRRLASKTQEAQ AGSQDMVAKLLAQSYEQQQEQEKLEREMALLRGAIEDQRRRAELLEQALG NAQGRAARAEEELRKKQAYVEKVERLQQALGQLQAACEKREQLELRLRTR LEQELKALRAQQRQAGAPGGSSGSGGSPELSALRLSEQLREKEEQILALE ADMTKWEQKYLEERAMRQFAMDAAATAAAQRDTTLIRHSPQPSPSSSFNE GLLTGGHRHQEMESRLKVLHAQILEKDAVIKVLQQRSRRDPGKAIQGSLR PAKSVPSVFAAAAAGTQGWQGLSSSERQTADAPARLTTDRAPTEEPVVTA PPAAHAKHGSRDGSTQTEGPPDSTSTCLPPEPDSLLGCSSSQRAASLDSV ATSRVQDLSDMVEILI |
| 预测分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于AMOTL2重组蛋白的3篇参考文献的简要信息：
1. **"Angiomotin-like protein 2 regulates endothelial cell migration through interaction with the TSC2/Tuberin complex"**
- **作者**: Lv, M., et al.
- **摘要**: 研究揭示了AMOTL2重组蛋白通过结合TSC2复合物调控内皮细胞迁移的机制。实验表明，AMOTL2的PDZ结构域对细胞极性建立和迁移至关重要，为血管生成研究提供新视角。
2. **"Structural insights into AMOTL2-mediated YAP inhibition in the Hippo pathway"**
- **作者**: Wells, R.G., et al.
- **摘要**: 通过X射线晶体学解析AMOTL2重组蛋白的结构，发现其N端区域直接结合Hippo通路效应蛋白YAP，抑制其核转位，阐明AMOTL2在肿瘤抑制中的结构基础。
3. **"Recombinant AMOTL2 modulates tight junction assembly by recruiting PP1 phosphatase to enhance cell-cell adhesion"**
- **作者**: Bratt, A., et al.
- **摘要**: 研究表明，纯化的AMOTL2重组蛋白通过募集蛋白磷酸酶PP1.促进上皮细胞紧密连接的形成，揭示了其在维持细胞屏障功能中的新作用机制。
注：以上文献信息为示例性质，实际文献需通过学术数据库检索确认。若需具体文章，建议使用关键词“AMOTL2 recombinant protein”在PubMed或Web of Science进一步查询。
**Background of AMOTL2 Recombinant Protein**
AMOTL2 (Angiomotin-like 2) is a member of the angiomotin protein family, which includes AMOT and AMOTL1. These proteins are characterized by conserved coiled-coil domains and play roles in cellular processes such as cell migration, polarity, and morphogenesis. AMOTL2 is widely expressed in tissues, including endothelial and epithelial cells, and is implicated in regulating cell-cell adhesion, cytoskeletal organization, and signaling pathways like the Hippo-YAP pathway, which controls organ size and tumor suppression.
The recombinant AMOTL2 protein is engineered using molecular cloning techniques, often expressed in systems like *E. coli* or mammalian cells to ensure proper folding and post-translational modifications. It is purified via affinity tags (e.g., His-tag) for experimental applications. Recombinant AMOTL2 serves as a critical tool for studying its interactions with binding partners, such as YAP/TAZ transcription factors, and its role in modulating signaling cascades.
Research highlights AMOTL2’s dual functionality: it can act as a tumor suppressor by inhibiting YAP/TAZ activity or promote cancer progression in certain contexts, depending on cellular localization and interaction networks. Its involvement in angiogenesis and vascular development has also been explored, linking it to diseases like cancer and retinopathy.
Studies using recombinant AMOTL2 have advanced understanding of its structural domains, phosphorylation sites, and regulatory mechanisms, offering potential therapeutic targets for diseases driven by dysregulated cell proliferation or migration.
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