| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | HACL1 |
| Uniprot No | Q9UJ83 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-578aa |
| 氨基酸序列 | MPDSNFAERS EEQVSGAKVI AQALKTQDVE YIFGIVGIPV TEIAIAAQQL GIKYIGMRNE QAACYAASAI GYLTSRPGVC LVVSGPGLIH ALGGMANANM NCWPLLVIGG SSERNQETMG AFQEFPQVEA CRLYTKFSAR PSSIEAIPFV IEKAVRSSIY GRPGACYVDI PADFVNLQVN VNSIKYMERC MSPPISMAET SAVCTAASVI RNAKQPLLII GKGAAYAHAE ESIKKLVEQY KLPFLPTPMG KGVVPDNHPY CVGAARSRAL QFADVIVLFG ARLNWILHFG LPPRYQPDVK FIQVDICAEE LGNNVKPAVT LLGNIHAVTK QLLEELDKTP WQYPPESKWW KTLREKMKSN EAASKELASK KSLPMNYYTV FYHVQEQLPR DCFVVSEGAN TMDIGRTVLQ NYLPRHRLDA GTFGTMGVGL GFAIAAAVVA KDRSPGQWII CVEGDSAFGF SGMEVETICR YNLPIILLVV NNNGIYQGFD TDTWKEMLKF QDATAVVPPM CLLPNSHYEQ VMTAFGGKGY FVQTPEELQK SLRQSLADTT KPSLINIMIE PQATRKAQDF HWLTRSNM |
| 预测分子量 | 63,7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于HACL1重组蛋白研究的模拟参考文献示例(实际文献需通过学术数据库验证)：
---
1. **文献名称**: *"Functional characterization of human 2-hydroxyacyl-CoA lyase 1 (HACL1) in peroxisomal phytanic acid α-oxidation"*
**作者**: Croes, K., et al.
**摘要**: 本研究通过在大肠杆菌中重组表达人源HACL1蛋白，验证其催化2-羟基植烷酰-CoA裂解反应的活性，揭示了HACL1在过氧化物酶体植烷酸α氧化途径中的关键作用，并发现其酶活性依赖硫胺素焦磷酸(TPP)辅因子。
2. **文献名称**: *"Crystal structure of human HACL1 reveals insights into substrate recognition and catalysis"*
**作者**: Wang, L., et al.
**摘要**: 通过X射线晶体学解析HACL1重组蛋白的三维结构，发现其TPP结合域与底物通道的关键氨基酸残基，阐明了HACL1催化2-羟基酰基-CoA裂解反应的分子机制，为相关代谢疾病的药物设计提供结构基础。
3. **文献名称**: *"HACL1 deficiency disrupts fatty acid homeostasis and induces neuroinflammation in a mouse model"*
**作者**: Ferdinandusse, S., et al.
**摘要**: 利用重组HACL1蛋白进行体外酶活补偿实验，结合HACL1敲除小鼠模型，证明HACL1功能缺失导致长链2-羟基脂肪酸累积，引发神经炎症和运动功能障碍，提示其与遗传性周围神经病变的关联。
---
**说明**：以上内容为示例性概括，实际文献需通过PubMed、Google Scholar等平台以"HACL1 recombinant protein"或"HACL1 enzymatic mechanism"为关键词检索。推荐结合具体研究方向筛选高被引论文或近期综述。
HACL1 (2-Hydroxyacyl-CoA Lyase 1) is a peroxisomal enzyme critical in the α-oxidation pathway, which metabolizes branched-chain fatty acids and certain xenobiotics that cannot undergo β-oxidation. It specifically cleaves 2-hydroxyacyl-CoA substrates into acyl-CoA and formate, a process essential for degrading phytanic acid—a branched-chain fatty acid derived from dietary sources. Mutations in HACL1 are linked to metabolic disorders like adult-onset Refsum disease, characterized by phytanic acid accumulation, neuropathy, and retinal degeneration.
Recombinant HACL1 protein is produced using heterologous expression systems (e.g., E. coli or mammalian cells) to study its structure, catalytic mechanism, and role in lipid metabolism. The purified enzyme often retains its cofactor-dependent activity, requiring thiamine pyrophosphate (TPP) and Mg²⁺ for function. Its crystal structure reveals a homodimeric organization with conserved TPP-binding domains, aiding in understanding substrate specificity and enzyme kinetics.
Research applications include investigating peroxisomal disorders, drug screening for enzyme modulators, and engineering metabolic pathways for biotechnology. Recombinant HACL1 also serves as a tool to explore interactions with other peroxisomal proteins, such as ABCD3 and AMACR, which coordinate lipid processing. Current studies focus on its potential therapeutic targeting to mitigate toxic metabolite buildup in genetic diseases or metabolic syndromes.
×
