| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PPP4R1 |
| Uniprot No | Q8TF05 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-950aa |
| 氨基酸序列 | MADLSLLQEDLQEDADGFGVDDYSSESDVIIIPSALDFVSQDEMLTPLGRLDKYAASENIFNRQMVARSLLDTLREVCDDERDCIAVLERISRLADDSEPTVRAELMEQVPHIALFCQENRPSIPYAFSKFLLPIVVRYLADQNNQVRKTSQAALLALLEQELIERFDVETKVCPVLIELTAPDSNDDVKTEAVAIMCKMAPMVGKDITERLILPRFCEMCCDCRMFHVRKVCAANFGDICSVVGQQATEEMLLPRFFQLCSDNVWGVRKACAECFMAVSCATCQEIRRTKLSALFINLISDPSRWVRQAAFQSLGPFISTFANPSSSGQYFKEESKSSEEMSVENKNRTRDQEAPEDVQVRPEDTPSDLSVSNSSVILENTMEDHAAEASGKPLGEISVPLDSSLLCTLSSESHQEAASNENDKKPGNYKSMLRPEVGTTSQDSALLDQELYNSFHFWRTPLPEIDLDIELEQNSGGKPSPEGPEEESEGPVPSSPNITMATRKELEEMIENLEPHIDDPDVKAQVEVLSAALRASSLDAHEETISIEKRSDLQDELDINELPNCKINQEDSVPLISDAVENMDSTLHYIHSDSDLSNNSSFSPDEERRTKVQDVVPQALLDQYLSMTDPSRAQTVDTEIAKHCAYSLPGVALTLGRQNWHCLRETYETLASDMQWKVRRTLAFSIHELAVILGDQLTAADLVPIFNGFLKDLDEVRIGVLKHLHDFLKLLHIDKRREYLYQLQEFLVTDNSRNWRFRAELAEQLILLLELYSPRDVYDYLRPIALNLCADKVSSVRWISYKLVSEMVKKLHAATPPTFGVDLINELVENFGRCPKWSGRQAFVFVCQTVIEDDCLPMDQFAVHLMPHLLTLANDRVPNVRVLLAKTLRQTLLEKDYFLASASCHQEAVEQTIMALQMDRDSDVKYFASIHPASTKISEDAMSTASSTY |
| 预测分子量 | 107 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于PPP4R1重组蛋白的模拟参考文献示例，供参考格式和内容：
1. **文献名称**：*Structural Characterization of the PPP4R1 Regulatory Subunit in the PP4 Phosphatase Complex*
**作者**：M. Tanaka et al.
**摘要**：本研究通过重组表达并纯化人源PPP4R1蛋白，结合X射线晶体学分析其与PP4催化亚基的相互作用，揭示了PPP4R1在复合体组装和底物识别中的关键结构域。
2. **文献名称**：*Recombinant PPP4R1 Facilitates DNA Repair via Modulating HDAC Activity*
**作者**：S. Patel et al.
**摘要**：利用昆虫细胞系统表达重组PPP4R1.发现其与组蛋白去乙酰化酶HDAC3结合，并通过调控PP4磷酸酶活性促进DNA损伤后的染色质重塑，增强同源重组修复效率。
3. **文献名称**：*Functional Analysis of PPP4R1 in NF-κB Signaling Using Recombinant Protein Assays*
**作者**：K. Kim et al.
**摘要**：通过大肠杆菌表达重组PPP4R1.体外实验证实其通过PP4复合体介导IκBα去磷酸化，负调控NF-κB信号通路，影响炎症反应。
4. **文献名称**：*Recombinant PPP4R1 Interaction with Aurora Kinase B in Mitotic Regulation*
**作者**：L. García-Rodríguez et al.
**摘要**：研究利用重组PPP4R1蛋白进行Pull-down实验，发现其与Aurora Kinase B直接互作，调控有丝分裂中纺锤体组装检查点的磷酸化状态。
**备注**：以上文献为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。
**Background of PPP4R1 Recombinant Protein**
PPP4R1 (Protein Phosphatase 4 Regulatory Subunit 1) is a critical regulatory component of the protein phosphatase 4 (PP4) complex, a serine/threonine phosphatase involved in diverse cellular processes, including DNA damage response, cell cycle regulation, and immune signaling. As a regulatory subunit, PPP4R1 modulates the substrate specificity, enzymatic activity, and subcellular localization of the catalytic subunit PPP4C, ensuring precise phosphatase activity in context-dependent pathways. Structurally, PPP4R1 contains conserved HEAT repeats, facilitating protein-protein interactions essential for PP4 complex assembly and recruitment to specific targets.
Recombinant PPP4R1 protein is engineered for in vitro studies to dissect its functional roles and mechanisms. Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), the recombinant protein retains post-translational modifications and structural integrity, enabling investigations into its interaction partners, such as components of the DNA repair machinery (e.g., γ-H2AX or RAD51) or signaling adaptors in NF-κB pathways. Researchers utilize PPP4R1 recombinant protein to explore its regulatory effects on PP4-mediated dephosphorylation events, particularly in DNA repair scenarios (e.g., homologous recombination) or apoptosis regulation.
Dysregulation of PPP4R1 has been implicated in pathologies, including cancer and neurodegenerative disorders, highlighting its therapeutic relevance. Recombinant PPP4R1 serves as a tool for drug screening, structural studies, and functional assays to identify small molecules or mutations altering PP4 activity. Its role in maintaining genomic stability and modulating stress responses continues to drive interest in both basic research and translational applications.
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