| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WWC3 |
| Uniprot No | Q9ULE0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1216aa |
| 氨基酸序列 | MPWLSGGRRRRRGQPREAPREPPPSAQPQREPPPAPPAAVPTPPAPSAPPPRARESAELPLPAGWEEARDYDGRVFYIDHNTRQTSWIDPRDRITKPLTFADCVGDELPLGWETVYDKQIGVYYMDHINKLTQIEDPREQWRREQERMLKEYLIVAQEALNAKKEIYQIKQQRFELAQEEYQQLHKMCEDDSRSYASSFSGYSTNTKYDPHQIKAEIASRRDRLSRLKRELTQMKQELQYKEKGVETLQEIDRKMSSTHTSYKLDEAQAIMSELRTIKKAICTGEKERRDLMHSLAKLTDSFKNSCSVTDSLVDFPHHVGVPGDAGVPQQFCDAGSQTDIIGEFVFDDKTRLVDRVRLNWQYEEARKRVANIQQQLARLDNESWPSTAEADRDRLQLIKEKEALLQELQLIIAQRRSAGDVARLEEERERLEEELRRARATSAQGATERILLQEKRNCLLMQLEEATRLTSYLQSQLKSLCASTLTVSSGSSRGSLASSRGSLASSRGSLSSVSFTDIYGLPQYEKPDAEGSQLLRFDLIPFDSLGRDAPFSEPPGPSGFHKQRRSLDTPQSLASLSSRSSLSSLSPPSSPLDTPFLPASRDSPLAQLADSCEGPGLGALDRLRAHASAMGDEDLPGMAALQPHGVPGDGEGPHERGPPPASAPVGGTVTLREDSAKRLERRARRISACLSDYSLASDSGVFEPLTKRNEDAEEPAYGDTASNGDPQIHVGLLRDSGSECLLVHVLQLKNPAGLAVKEDCKVHIRVYLPPLDSGTPNTYCSKALEFQVPLVFNEVFRIPVHSSALTLKSLQLYVCSVTPQLQEELLGIAQINLADYDSLSEMQLRWHSVQVFTSSEPSRTREAGCAGESSARDPAHTISISGKTDAVTVLLARTTAQLQAVERELAEERAKLEYTEEEVLEMERKEEQAEAISERSWQADSVDSGCSNCTQTSPPYPEPCCMGIDSILGHPFAAQAGPYSPEKFQPSPLKVDKETNTEDLFLEEAASLVKERPSRRARGSPFVRSGTIVRSQTFSPGARSQYVCRLYRSDSDSSTLPRKSPFVRNTLERRTLRYKQSCRSSLAELMARTSLDLELDLQASRTRQRQLNEELCALRELRQRLEDAQLRGQTDLPPWVLRDERLRGLLREAERQTRQTKLDYRHEQAAEKMLKKASKEIYQLRGQSHKEPIQVQTFREKIAFFTRPRINIPPLPADDV |
| 预测分子量 | 136,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WWC3重组蛋白的3篇参考文献示例(注：部分信息为虚构示例，实际文献需根据具体研究查询)：
1. **文献名称**: "Cloning and Expression of Recombinant WWC3 Protein in E. coli"
**作者**: Zhang L, et al.
**摘要**: 本研究成功构建了WWC3基因的原核表达载体，并利用大肠杆菌系统高效表达可溶性重组WWC3蛋白，通过亲和层析纯化获得高纯度蛋白，为后续功能研究奠定基础。
2. **文献名称**: "Structural Insights into WWC3 Protein and Its Role in Hippo Signaling Pathway"
**作者**: Tanaka K, et al.
**摘要**: 通过X射线晶体学解析了WWC3蛋白的WW结构域三维结构，揭示其与Hippo通路关键蛋白(如LATS1/2)的相互作用机制，证明WWC3通过调控YAP/TAZ活性影响细胞增殖。
3. **文献名称**: "Functional Characterization of Recombinant WWC3 in Cell Adhesion and Migration"
**作者**: Chen X, et al.
**摘要**: 利用哺乳动物细胞表达系统获得重组WWC3蛋白，发现其通过结合细胞骨架相关蛋白(如AMOT)调控细胞黏附和迁移，提示WWC3在肿瘤转移中的潜在作用。
**注意**：以上文献为示例，实际研究中请通过PubMed、Web of Science等平台检索最新文献。若WWC3研究较少，可扩展关键词至“WW domain-containing protein”或“Hippo pathway recombinant proteins”。
**Background of WWC3 Recombinant Protein**
WWC3 (WW Domain-Containing Protein 3), also known as KIBRA, is a member of the WWC protein family, which includes WWC1 and WWC2. These proteins share conserved structural features, including two WW domains (protein-protein interaction modules) and a C2 domain (involved in lipid binding). WWC3 plays a critical role in regulating the Hippo signaling pathway, a conserved pathway that controls organ size, tissue regeneration, and tumor suppression by balancing cell proliferation and apoptosis.
Functionally, WWC3 acts as a scaffold protein, facilitating interactions between core Hippo pathway components, such as MST1/2 kinases and LATS1/2 kinases. By promoting LATS1/2 activation, WWC3 enhances phosphorylation and inactivation of the oncogenic transcriptional co-activators YAP (Yes-associated protein) and TAZ (Transcriptional co-activator with PDZ-binding motif), thereby suppressing their nuclear translocation and pro-growth signaling. Dysregulation of WWC3 has been implicated in cancer progression, as reduced expression correlates with YAP/TAZ hyperactivation, tumor cell survival, and metastasis.
Recombinant WWC3 protein is engineered for in vitro and in vivo studies to dissect its molecular mechanisms, therapeutic potential, and interactions within the Hippo pathway. Produced using expression systems like *E. coli* or mammalian cells, it retains functional domains for binding partners and enzymatic assays. Its applications span basic research (e.g., pathway modulation, structural studies) and drug discovery (e.g., screening for Hippo-targeted therapies). WWC3 recombinant protein thus serves as a vital tool for exploring tissue homeostasis, cancer biology, and regenerative medicine.
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