| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | WWC2 |
| Uniprot No | Q6AWC2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1192aa |
| 氨基酸序列 | MPRRAGSGQLPLPRGWEEARDYDGKVFYIDHNTRRTSWIDPRDRLTKPLSFADCVGDELPWGWEAGFDPQIGVYYIDHINKTTQIEDPRKQWRGEQEKMLKDYLSVAQDALRTQKELYHVKEQRLALALDEYVRLNDAYKEKSSSHTSLFSGSSSSTKYDPDILKAEISTTRLRVKKLKRELSQMKQELLYKEQGFETLQQIDKKMSGGQSGYELSEAKAILTELKSIRKAISSGEKEKQDLMQSLAKLQERFHLDQNIGRSEPDLRCSPVNSHLCLSRQTLDAGSQTSISGDIGVRSRSNLAEKVRLSLQYEEAKRSMANLKIELSKLDSEAWPGALDIEKEKLMLINEKEELLKELQFVTPQKRTQDELERLEAERQRLEEELLSVRGTPSRALAERLRLEERRKELLQKLEETTKLTTYLHSQLKSLSASTLSMSSGSSLGSLASSRGSLNTSSRGSLNSLSSTELYYSSQSDQIDVDYQYKLDFLLQEKSGYIPSGPITTIHENEVVKSPSQPGQSGLCGVAAAATGHTPPLAEAPKSVASLSSRSSLSSLSPPGSPLVLEGTFPMSSSHDASLHQFTADFEDCELSSHFADISLIENQILLDSDSGGASQSLSEDKDLNECAREPLYEGTADVEKSLPKRRVIHLLGEKTTCVSAAVSDESVAGDSGVYEAFVKQPSEMEDVTYSEEDVAIVETAQVQIGLRYNAKSSSFMVIIAQLRNLHAFLIPHTSKVYFRVAVLPSSTDVSCLFRTKVHPPTESILFNDVFRVAISQTALQQKTLRVDLCSVSKHRREECLAGTQISLADLPFSSEVFTLWYNLLPSKQMPCKKNEENEDSVFQPNQPLVDSIDLDAVSALLARTSAELLAVEQELAQEEEEESGQEEPRGPDGDWLTMLREASDEIVAEKEAEVKLPEDSSCTEDLSSCTSVPEMNEDGNRKESNCAKDLRSQPPTRIPTLVDKETNTDEAANDNMAVRPKERSSLSSRQHPFVRSSVIVRSQTFSPGERNQYICRLNRSDSDSSTLAKKSLFVRNSTERRSLRVKRTVCQSVLRRTTQECPVRTSLDLELDLQASLTRQSRLNDELQALRDLRQKLEELKAQGETDLPPGVLEDERFQRLLKQAEKQAEQSKEEQKQGLNAEKLMRQVSKDVCRLREQSQKVPRQVQSFREKIAYFTRAKISIPSLPADDV |
| 预测分子量 | 133,8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于WWC2重组蛋白的3篇参考文献(基于已有研究背景的合理推测，若需准确信息请通过学术数据库核实)：
1. **文献名称**：*WWC2 regulates cell cycle arrest and apoptosis through the Hippo pathway in breast cancer*
**作者**：Li, Y., et al.
**摘要**：研究利用重组WWC2蛋白验证其与LATS1激酶的结合，证实WWC2通过激活Hippo通路抑制乳腺癌细胞增殖并诱导凋亡。
2. **文献名称**：*Structural basis of WW domain-mediated interactions in the Hippo pathway*
**作者**：Sudol, M., et al.
**摘要**：通过重组表达WWC2的WW结构域，结合晶体结构分析揭示其与PPxY基序蛋白(如TAZ)的相互作用机制，为靶向药物设计提供依据。
3. **文献名称**：*WWC2 suppresses colorectal cancer metastasis by modulating EGFR signaling*
**作者**：Wang, H., et al.
**摘要**：研究使用重组WWC2蛋白体外验证其抑制EGFR内吞的作用，阐明WWC2通过阻断EGFR/MAPK通路抑制结直肠癌转移的分子机制。
注：以上文献信息为模拟示例，实际引用时请以具体论文数据为准。
WWC2 (WW and C2 domain-containing protein 2), also known as KIBRA, is a member of the WWC protein family implicated in regulating cell proliferation, migration, and organ size through the Hippo signaling pathway. This conserved pathway controls tissue homeostasis by balancing cell growth and apoptosis. WWC2 acts as a scaffold protein, facilitating interactions between core Hippo pathway components such as LATS1/2 kinases and SAV1. which phosphorylate and inhibit the transcriptional co-activators YAP/TAZ. Dysregulation of WWC2 or Hippo signaling is linked to cancer, neurodegeneration, and developmental disorders.
Structurally, WWC2 contains two WW domains that mediate protein-protein interactions, a C2 domain involved in membrane binding, and a coiled-coil region for oligomerization. Its expression is enriched in the brain, kidneys, and mammary glands. Research highlights WWC2's dual roles: as a tumor suppressor in breast and liver cancers by restricting YAP/TAZ activity, and as a regulator of synaptic plasticity and memory formation in neurons.
Recombinant WWC2 protein, typically produced in Escherichia coli or mammalian expression systems, enables functional studies of its biochemical properties, post-translational modifications, and binding partners. It serves as a tool to investigate Hippo signaling mechanisms, screen for therapeutic compounds targeting this pathway, and explore WWC2's involvement in diseases. Recent studies also suggest its potential in modulating cell polarity and 3D tissue organization, making it relevant for regenerative medicine applications.
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