| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHb10 |
| Uniprot No | Q9UN67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-800aa |
| 氨基酸序列 | MAVRELCFPRQRQVLFLFLFWGVSLAGSGFGRYSVTEETEKGSFVVNLAKDLGLAEGELAARGTRVVSDDNKQYLLLDSHTGNLLTNEKLDREKLCGPKEPCMLYFQILMDDPFQIYRAELRVRDINDHAPVFQDKETVLKISENTAEGTAFRLERAQDPDGGLNGIQNYTISPNSFFHINISGGDEGMIYPELVLDKALDREEQGELSLTLTALDGGSPSRSGTSTVRIVVLDVNDNAPQFAQALYETQAPENSPIGFLIVKVWAEDVDSGVNAEVSYSFFDASENIRTTFQINPFSGEIFLRELLDYELVNSYKINIQAMDGGGLSARCRVLVEVLDTNDNPPELIVSSFSNSVAENSPETPLAVFKINDRDSGENGKMVCYIQENLPFLLKPSVENFYILITEGALDREIRAEYNITITVTDLGTPRLKTEHNITVLVSDVNDNAPAFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVTYSLLPPQDPHLPLASLVSINADNGHLFALRSLDYEALQAFEFRVGATDRGSPALSREALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHLLLVDGFSQPYLPLPEAAPAQAQAEADLLTVYLVVALASVSSLFLLSVLLFVAVRLCRRSRAASVGRCSVPEGPFPGHLVDVRGAETLSQSYQYEVCLTGGPGTSEFKFLKPVISDIQAQGPGRKGEENSTFRNSFGFNIQ |
| 预测分子量 | 87,6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇与PCDHb10(原钙粘蛋白β10)重组蛋白相关的参考文献示例(内容为模拟概括，建议通过学术数据库验证)：
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1. **文献名称**: "Structural characterization of recombinant protocadherin-β10 extracellular domain and its role in neuronal adhesion"
**作者**: Li X, et al.
**摘要**: 本研究通过哺乳动物细胞系统表达并纯化了PCDHb10的胞外结构域重组蛋白，解析了其晶体结构。实验证明该蛋白通过同源相互作用介导神经元粘附，为原钙粘蛋白家族在神经回路特异性中的作用提供证据。
2. **文献名称**: "Recombinant PCDHb10 promotes synaptic plasticity in vitro via Wnt signaling pathway"
**作者**: Wang Y, et al.
**摘要**: 文章报道了利用昆虫表达系统制备功能性PCDHb10重组蛋白，发现其可通过激活Wnt/β-catenin信号通路增强体外培养神经元的突触可塑性，提示其在神经发育疾病中的潜在治疗价值。
3. **文献名称**: "High-yield expression and purification of PCDHb10 for antibody production and epitope mapping"
**作者**: Kimura T, et al.
**摘要**: 本研究优化了PCDHb10重组蛋白在大肠杆菌中的可溶性表达方案，并利用该蛋白成功制备了单克隆抗体，为原钙粘蛋白家族的功能研究和诊断试剂开发提供了技术基础。
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**注意**：以上文献信息为示例性概括，实际研究请通过PubMed、Web of Science或Google Scholar以“PCDHb10 recombinant protein”“protocadherin beta 10”等关键词检索最新文献。
**Background of PCDHb10 Recombinant Protein**
Protocadherin beta-10 (PCDHb10) is a member of the protocadherin beta gene cluster, part of the cadherin superfamily known for mediating calcium-dependent cell-cell adhesion. These transmembrane proteins play critical roles in neural development, synaptic specificity, and maintaining tissue architecture. PCDHb10. specifically, is implicated in neuronal circuit formation, synaptic plasticity, and cellular identity determination during brain development. Its extracellular domain contains multiple cadherin repeats that facilitate homophilic interactions, while the intracellular domain interacts with cytoskeletal components and signaling molecules, influencing cell signaling pathways.
Research highlights PCDHb10's involvement in cancer biology, where its dysregulation is linked to tumor progression, metastasis, and drug resistance. For instance, aberrant expression has been observed in certain cancers, suggesting roles in epithelial-mesenchymal transition (EMT) or cell survival signaling. In neuroscience, PCDHb10 contributes to establishing complex neural networks, with studies implicating it in neurodevelopmental disorders.
Recombinant PCDHb10 protein is engineered using expression systems (e.g., mammalian, insect, or bacterial cells) to produce purified, functional domains or full-length proteins for experimental applications. This protein serves as a tool to study its biological functions, ligand interactions, and structural properties in vitro. It is widely used in antibody development, binding assays, and mechanistic studies exploring its role in diseases. The recombinant form allows researchers to bypass challenges associated with native protein isolation, offering consistent quality and scalability.
Overall, PCDHb10 recombinant protein is a vital resource for deciphering the molecular basis of neural development and cancer, with potential therapeutic implications.
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