| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | KPNa4 |
| Uniprot No | O00629 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-521aa |
| 氨基酸序列 | MADNEKLDNQRLKNFKNKGRDLETMRRQRNEVVVELRKNKRDEHLLKRRN VPHEDICEDSDIDGDYRVQNTSLEAIVQNASSDNQGIQLSAVQAARKLLS SDRNPPIDDLIKSGILPILVHCLERDDNPSLQFEAAWALTNIASGTSEQT QAVVQSNAVPLFLRLLHSPHQNVCEQAVWALGNIIGDGPQCRDYVISLGV VKPLLSFISPSIPITFLRNVTWVMVNLCRHKDPPPPMETIQEILPALCVL IHHTDVNILVDTVWALSYLTDAGNEQIQMVIDSGIVPHLVPLLSHQEVKV QTAALRAVGNIVTGTDEQTQVVLNCDALSHFPALLTHPKEKINKEAVWFL SNITAGNQQQVQAVIDANLVPMIIHLLDKGDFGTQKEAAWAISNLTISGR KDQVAYLIQQNVIPPFCNLLTVKDAQVVQVVLDGLSNILKMAEDEAETIG NLIEECGGLEKIEQLQNHENEDIYKLAYEIIDQFFSSDDIDEDPSLVPEA IQGGTFGFNSSANVPTEGFQF |
| 预测分子量 | 83 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于KPNa4(Karyopherin alpha 4)重组蛋白的3篇代表性文献示例(注：文献为模拟示例，实际检索请参考具体数据库)：
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1. **文献名称**：*"Efficient bacterial expression and purification of human KPNA4 for structural studies"*
**作者**：Miller, A. et al.
**摘要**：本研究开发了一种在大肠杆菌中高效表达重组人KPNA4蛋白的方法，采用His标签纯化策略，并通过圆二色光谱验证其正确折叠。研究为后续结构功能分析提供了高质量蛋白样本。
2. **文献名称**：*"KPNA4 mediates nuclear import of SARS-CoV-2 ORF6 via recombinant protein interaction assays"*
**作者**：Chen, L. et al.
**摘要**：通过重组KPNA4蛋白与新冠病毒ORF6蛋白的体外结合实验，揭示了KPNA4在病毒蛋白入核机制中的关键作用，为抗病毒药物靶点提供了依据。
3. **文献名称**：*"Cryo-EM structure of the KPNA4-Importin β complex reveals cargo recognition specificity"*
**作者**：Yamamoto, T. et al.
**摘要**：利用冷冻电镜解析了重组KPNA4与Importin β的复合物结构，阐明了KPNA4识别不同核定位信号(NLS)的分子基础，解释了其在细胞分化中的选择性转运功能。
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**备注**：若需真实文献，建议在PubMed或Google Scholar中检索关键词 **"KPNA4 recombinant"** 或 **"Importin alpha 4 expression"**，可筛选出涉及蛋白表达、结构解析及功能机制的研究。部分研究可能侧重其在癌症、病毒感染或神经疾病中的病理意义。
**Background of KPNa4 Recombinant Protein**
KPNa4. a member of the Karyopherin-α (importin α) family, is a critical regulator of nucleocytoplasmic transport. It functions as an adaptor protein that recognizes nuclear localization signals (NLS) on cargo proteins, facilitating their import into the nucleus via the nuclear pore complex (NPC) in collaboration with Karyopherin-β (importin β). The Karyopherin-α family comprises multiple isoforms, with KPNa4 playing distinct roles in cellular processes such as gene expression regulation, cell cycle progression, and stress responses. Its expression and activity are tightly linked to developmental processes and disease states, including cancer and viral infections.
Recombinant KPNa4 protein is typically produced using heterologous expression systems, such as *E. coli* or mammalian cell cultures, followed by purification via affinity chromatography. This engineered protein retains the ability to bind NLS-containing cargoes, making it a valuable tool for studying nuclear transport mechanisms *in vitro*. Structurally, KPNa4 contains conserved Armadillo (ARM) repeat domains that mediate NLS recognition and a flexible N-terminal region regulating interactions with importin β.
Research applications of KPNa4 recombinant protein include elucidating nuclear import pathways, dissecting molecular interactions in transport assays, and screening for inhibitors targeting pathogenic nuclear trafficking (e.g., viral replication or oncoprotein dysregulation). Its role in diseases has spurred interest in therapeutic targeting, particularly in cancers where aberrant nuclear transport drives tumor progression. Additionally, KPNa4 serves as a reference protein in comparative studies of importin α isoforms to uncover isoform-specific functions.
Overall, KPNa4 recombinant protein bridges fundamental insights into cellular trafficking with translational opportunities in drug discovery and disease biology.
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