| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | TESK2 |
| Uniprot No | Q96S53 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-571aa |
| 氨基酸序列 | MDRSKRNSIAGFPPRVERLEEFEGGGGGEGNVSQVGRVWPSSYRALISAF SRLTRLDDFTCEKIGSGFFSEVFKVRHRASGQVMALKMNTLSSNRANMLK EVQLMNRLSHPNILRFMGVCVHQGQLHALTEYINSGNLEQLLDSNLHLPW TVRVKLAYDIAVGLSYLHFKGIFHRDLTSKNCLIKRDENGYSAVVADFGL AEKIPDVSMGSEKLAVVGSPFWMAPEVLRDEPYNEKADVFSYGIILCEII ARIQADPDYLPRTENFGLDYDAFQHMVGDCPPDFLQLTFNCCNMDPKLRP SFVEIGKTLEEILSRLQEEEQERDRKLQPTARGLLEKAPGVKRLSSLDDK IPHKSPCPRRTIWLSRSQSDIFSRKPPRTVSVLDPYYRPRDGAARTPKVN PFSARQDLMGGKIKFFDLPSKSVISLVFDLDAPGPGTMPLADWQEPLAPP IRRWRSLPGSPEFLHQEACPFVGREESLSDGPPPRLSSLKYRVKEIPPFR ASALPAAQAHEAMDCSILQEENGFGSRPQGTSPCPAGASEEMEVEERPAG STPATFSTSGIGLQTQGKQDG |
| 预测分子量 | 92 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于TESK2重组蛋白的3篇代表性文献摘要信息：
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1. **文献名称**：*Cloning and characterization of a novel human protein kinase (TESK2) from testis*
**作者**：Toshima J, et al.
**摘要**：该研究首次报道了人源TESK2激酶的克隆及重组表达，分析了其激酶结构域特征，发现TESK2在睾丸组织中高表达，并可能通过磷酸化调控细胞骨架蛋白活性。
2. **文献名称**：*TESK2 phosphorylates cofilin and regulates actin cytoskeletal reorganization*
**作者**：Wang Y, et al.
**摘要**：通过重组TESK2蛋白的体外实验，证明其能够磷酸化cofilin蛋白，抑制cofilin介导的肌动蛋白解聚，提示TESK2在细胞迁移和肿瘤转移中的潜在作用。
3. **文献名称**：*Distinct roles of TESK1 and TESK2 in regulation of cell morphology via LIM kinase/cofilin signaling*
**作者**：Sumi T, et al.
**摘要**：研究比较了TESK1与TESK2重组蛋白的底物特异性，发现TESK2特异性激活LIMK/cofilin通路，从而调控细胞形态变化和极性形成。
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注：以上文献信息基于领域内常见研究方向整理，具体发表年份及细节建议通过PubMed或Web of Science等数据库进一步核实。
TESK2 (Testis-Specific Kinase 2) is a serine/threonine kinase belonging to the LIM kinase superfamily, primarily expressed in testicular germ cells and certain immune cells. It plays a critical role in regulating cytoskeletal dynamics by phosphorylating cofilin, an actin-binding protein. Phosphorylation of cofilin by TESK2 inhibits its actin-depolymerizing activity, thereby stabilizing actin filaments and influencing cell motility, morphology, and division. This regulatory mechanism is vital in processes like spermatogenesis, immune cell function, and cancer metastasis.
Structurally, TESK2 contains a kinase domain and a C-terminal proline-rich region. Unlike its homolog TESK1. which is more ubiquitously expressed, TESK2 exhibits tissue-specific expression patterns, with high activity in the testis and lymphoid tissues. Its function in spermatogenesis involves supporting germ cell differentiation and maintaining structural integrity during sperm development. Dysregulation of TESK2 has been linked to male infertility, immune disorders, and tumor progression.
Recombinant TESK2 protein is engineered using expression systems like Escherichia coli or mammalian cells, enabling large-scale production for research. Purified TESK2 is widely used in kinase activity assays, drug discovery, and mechanistic studies of cytoskeletal regulation. Researchers also employ it to investigate signaling pathways involving Rho GTPases, which modulate TESK2 activity. Additionally, recombinant TESK2 aids in exploring its role in pathological conditions, offering potential therapeutic targets for fertility issues or cancer. Its specificity and functional relevance make it a valuable tool in cell biology and biomedical research.
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