| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RBX1 |
| Uniprot No | P62877 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-108aa |
| 氨基酸序列 | MAAAMDVDTPSGTNSGAGKKRFEVKKWNAVALWAWDIVVDNCAICRNHIMDLCIECQANQASATSEECTVAWGVCNHAFHFHCISRWLKTRQVCPLDNREWEFQKYGH |
| 预测分子量 | 43.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RBX1重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*Structure of the Cul1–Rbx1–Skp1–F boxSkp2 SCF ubiquitin ligase complex*
**作者**：Zheng, N., Schulman, B.A., et al.
**摘要**：该研究通过X射线晶体学解析了Cul1-Rbx1-Skp1-F box复合物的三维结构，揭示了RBX1作为RING结构域蛋白在泛素连接酶复合物中的关键作用，其重组蛋白形式被用于验证其促进Cullin家族成员泛素化底物的机制。
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2. **文献名称**：*An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer*
**作者**：Soucy, T.A., Smith, P.G., et al.
**摘要**：本文报道了MLN4924药物通过抑制RBX1依赖的NEDD8活化酶，阻断Cullin-RING连接酶(CRL)的活性，从而抑制肿瘤细胞增殖。研究中利用重组RBX1蛋白验证了其与NEDD8的相互作用及药物靶向机制。
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3. **文献名称**：*Structural basis of the Cullin–RING ligase architecture*
**作者**：Hao, B., Oehlmann, S., et al.
**摘要**：该研究利用重组RBX1蛋白与不同Cullin家族成员进行体外组装，结合冷冻电镜技术阐明了CRL复合物的动态构象变化，强调了RBX1在泛素转移过程中协调E2酶活性的核心功能。
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如需更多文献或扩展内容，可进一步补充说明。
**Background of RBX1 Recombinant Protein**
RBX1 (RING-box protein 1), also known as ROC1. is a critical component of the Cullin-RING ligase (CRL) family of E3 ubiquitin ligases. These enzymes play a central role in the ubiquitin-proteasome system (UPS), mediating the transfer of ubiquitin molecules to substrate proteins, marking them for proteasomal degradation. RBX1 contains a RING-H2 domain that facilitates interaction with E2 ubiquitin-conjugating enzymes, enabling the transfer of ubiquitin to target substrates. Structurally, RBX1 forms a heterodimeric complex with Cullin proteins (e.g., CUL1. CUL2), serving as a scaffold to recruit substrate-specific adaptors and E2 enzymes, thereby defining substrate specificity.
RBX1 is essential for cellular processes such as cell cycle regulation, DNA damage response, and signal transduction. Dysregulation of RBX1 has been implicated in various pathologies, including cancer, neurodegenerative diseases, and immune disorders. For instance, overexpression of RBX1 in tumors correlates with enhanced ubiquitination and degradation of tumor suppressors, promoting oncogenesis. Conversely, RBX1 deficiency disrupts CRL activity, leading to accumulation of cell cycle inhibitors and impaired proliferation.
Recombinant RBX1 protein is engineered for research applications, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells) to ensure high purity and activity. Its production enables mechanistic studies of CRL assembly, substrate recognition, and ubiquitination kinetics. Additionally, recombinant RBX1 is used in drug discovery to screen for inhibitors targeting E3 ligase activity, offering therapeutic potential for CRL-dependent diseases.
In summary, RBX1 is a pivotal regulator of protein homeostasis, and its recombinant form serves as a vital tool for dissecting UPS mechanisms and developing targeted therapies.
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