首页 / 产品 / 蛋白 / 细胞因子、趋化因子与生长因子
| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PLT |
| Uniprot No | Q02083 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-359aa |
| 氨基酸序列 | MRTADREARPGLPSLLLLLLAGAGLSAASPPAAPRFNVSLDSVPELRWLPVLRHYDLDLVRAAMAQVIGDRVPKWVHVLIGKVVLELERFLPQPFTGEIRGMCDFMNLSLADCLLVNLAYESSVFCTSIVAQDSRGHIYHGRNLDYPFGNVLRKLTVDVQFLKNGQIAFTGTTFIGYVGLWTGQSPHKFTVSGDERDKGWWWENAIAALFRRHIPVSWLIRATLSESENFEAAVGKLAKTPLIADVYYIVGGTSPREGVVITRNRDGPADIWPLDPLNGAWFRVETNYDHWKPAPKEDDRRTSAIKALNATGQANLSLEALFQILSVVPVYNNFTIYTTVMSAGSPDKYMTRIRNPSRK |
| 预测分子量 | 40 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3-4条关于PLT(拟南芥PEBP家族转录因子)重组蛋白的参考文献示例及简要摘要内容：
1. **文献名称**：*Recombinant PLT Proteins Induce Stem Cell Activity in Plant Root Regeneration*
**作者**：Shimizu et al.
**摘要**：本研究通过大肠杆菌表达系统成功制备重组PLT蛋白，并证明其在体外能够激活植物根尖干细胞分化，为植物组织再生技术提供新方法。
2. **文献名称**：*Structural Insights into PLT Transcription Factors by X-ray Crystallography*
**作者**：Kumar & Müller
**摘要**：利用重组PLT2蛋白进行X射线晶体学分析，解析其三维结构，揭示其与DNA结合的分子机制，为调控植物发育的基因工程奠定基础。
3. **文献名称**：*PLT Recombinant Protein Enhances Drought Tolerance in Crops*
**作者**：Gonzalez et al.
**摘要**：通过转基因技术表达重组PLT4蛋白，显著提高作物根系发育和耐旱性，证明其在农业生物技术中的潜在应用价值。
4. **文献名称**：*Functional Crosstalk Between PLT Proteins and Auxin Signaling*
**作者**：Chen et al.
**摘要**：重组PLT3蛋白与生长素信号通路相互作用的研究，表明PLT家族通过调控激素响应基因参与植物根系的形态建成。
(注：以上文献为示例性概括，实际引用时需查询真实发表的论文。)
**Background of PLT Recombinant Proteins**
PLT (PLETHORA) recombinant proteins are derived from a family of transcription factors initially identified in plants, notably *Arabidopsis thaliana*, where they play pivotal roles in regulating stem cell maintenance, root development, and organ formation. Structurally, PLT proteins contain AP2 DNA-binding domains, enabling them to interact with specific gene promoters to modulate developmental processes. Their expression gradients, established by auxin signaling, spatially coordinate cellular differentiation and tissue patterning.
Functionally, PLT proteins act as master regulators of plant growth, influencing root meristem activity, vascular development, and stress responses. Studies highlight their role in enhancing regenerative capacity, making them critical targets for plant biotechnology. Recombinant PLT proteins are produced using heterologous expression systems (e.g., *E. coli*, yeast, or mammalian cells*) to ensure high purity and scalability. These systems allow researchers to study PLT mechanisms *in vitro* or apply them exogenously to manipulate plant growth.
Applications span basic research and agriculture. For instance, recombinant PLT proteins are used to improve root architecture in crops, boost stress resilience, or accelerate tissue culture regeneration. They also serve as tools to dissect signaling pathways or engineer synthetic gene circuits in synthetic biology. Challenges include optimizing protein stability and delivery methods in plants, as well as ensuring cost-effective production. Advances in protein engineering and CRISPR-based gene editing may further expand their utility, offering sustainable solutions for crop improvement and biomanufacturing. Overall, PLT recombinant proteins bridge molecular biology and agrotechnology, underscoring their potential in addressing global agricultural challenges.
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