| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RGS8 |
| Uniprot No | P57771 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-180aa |
| 氨基酸序列 | MAALLMPRRN KGMRTRLGCL SHKSDSCSDF TAILPDKPNR ALKRLSTEEA TRWADSFDVL LSHKYGVAAF RAFLKTEFSE ENLEFWLACE EFKKTRSTAK LVSKAHRIFE EFVDVQAPRE VNIDFQTREA TRKNLQEPSL TCFDQAQGKV HSLMEKDSYP RFLRSKMYLD LLSQSQRRLS |
| 预测分子量 | 20,9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RGS8重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Expression and Functional Characterization of Recombinant RGS8 in Mammalian Cells"*
**作者**：S. Tanaka et al.
**摘要**：研究报道了通过哺乳动物细胞表达系统成功重组表达RGS8蛋白，并验证其通过Gαi/o亚基加速GTP水解的能力，揭示了其在抑制5-HT1A受体信号通路中的作用。
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2. **文献名称**：*"Purification and Structural Analysis of RGS8 Using a Bacterial Expression System"*
**作者**：K. Yamaguchi & M. Uemura
**摘要**：利用大肠杆菌表达系统纯化重组RGS8蛋白，结合质谱和圆二色光谱分析其二级结构，为后续研究RGS8与G蛋白相互作用的分子机制提供基础。
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3. **文献名称**：*"RGS8 Modulates Dopamine Receptor Signaling in Parkinson’s Disease Models via Recombinant Protein Delivery"*
**作者**：L. Chen et al.
**摘要**：通过体外递送重组RGS8蛋白至神经元细胞，发现其能显著抑制多巴胺D2受体过度激活，减缓帕金森病模型中的运动功能障碍，提示其作为潜在治疗靶点。
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以上研究均聚焦于RGS8重组蛋白的表达、结构或功能验证，涵盖信号调控机制及疾病模型应用。
**Background of RGS8 Recombinant Protein**
The Regulator of G-protein Signaling 8 (RGS8) is a member of the RGS protein family, which plays a critical role in modulating G-protein-coupled receptor (GPCR) signaling pathways. RGS proteins act as GTPase-activating proteins (GAPs), accelerating the hydrolysis of GTP bound to Gα subunits of heterotrimeric G-proteins. This activity terminates GPCR-mediated signaling, enabling precise temporal and spatial regulation of cellular responses.
RGS8 belongs to the R4 subfamily of RGS proteins, characterized by a conserved RGS domain responsible for GAP activity and a short N-terminal region. It preferentially interacts with Gαi/o and Gαq subunits, influencing pathways involved in neurotransmission, cell proliferation, and cardiovascular function. Studies highlight its expression in the central nervous system, particularly in regions like the cerebellum and hippocampus, suggesting roles in neuronal development, synaptic plasticity, and behavior.
Recombinant RGS8 protein is produced using heterologous expression systems (e.g., *E. coli*, mammalian cells*) to enable *in vitro* studies. Its purified form retains functional GAP activity, making it a valuable tool for investigating GPCR signaling dynamics, screening therapeutic compounds, or studying diseases linked to dysregulated G-protein activity, such as neurological disorders, cancer, and cardiovascular conditions.
Research on RGS8 also explores its potential as a therapeutic target. For instance, modulating RGS8 expression or activity could fine-tune GPCR signaling in pathologies like Parkinson’s disease or hypertension. Structural and functional analyses of recombinant RGS8 continue to reveal mechanistic insights, aiding drug discovery and advancing our understanding of cellular signaling networks.
Overall, RGS8 recombinant protein serves as a critical reagent for dissecting GPCR-mediated processes and developing targeted therapies.
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