| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SOX5 |
| Uniprot No | P35711 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-763aa |
| 氨基酸序列 | MLTDPDLPQE FERMSSKRPA SPYGEADGEV AMVTSRQKVE EEESDGLPAF HLPLHVSFPN KPHSEEFQPV SLLTQETCGH RTPTSQHNTM EVDGNKVMSS FAPHNSSTSP QKAEEGGRQS GESLSSTALG TPERRKGSLA DVVDTLKQRK MEELIKNEPE ETPSIEKLLS KDWKDKLLAM GSGNFGEIKG TPESLAEKER QLMGMINQLT SLREQLLAAH DEQKKLAASQ IEKQRQQMEL AKQQQEQIAR QQQQLLQQQH KINLLQQQIQ VQGQLPPLMI PVFPPDQRTL AAAAQQGFLL PPGFSYKAGC SDPYPVQLIP TTMAAAAAAT PGLGPLQLQQ LYAAQLAAMQ VSPGGKLPGI PQGNLGAAVS PTSIHTDKST NSPPPKSKDE VAQPLNLSAK PKTSDGKSPT SPTSPHMPAL RINSGAGPLK ASVPAALASP SARVSTIGYL NDHDAVTKAI QEARQMKEQL RREQQVLDGK VAVVNSLGLN NCRTEKEKTT LESLTQQLAV KQNEEGKFSH AMMDFNLSGD SDGSAGVSES RIYRESRGRG SNEPHIKRPM NAFMVWAKDE RRKILQAFPD MHNSNISKIL GSRWKAMTNL EKQPYYEEQA RLSKQHLEKY PDYKYKPRPK RTCLVDGKKL RIGEYKAIMR NRRQEMRQYF NVGQQAQIPI ATAGVVYPGA IAMAGMPSPH LPSEHSSVSS SPEPGMPVIQ STYGVKGEEP HIKEEIQAED INGEIYDEYD EEEDDPDVDY GSDSENHIAG QAN |
| 预测分子量 | 84 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于SOX5重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**: "SOX5 controls the sequential generation of distinct corticofugal neuron subtypes"
**作者**: Kwan, K.Y., Sestan, N., Anton, E.S.
**摘要**: 该研究利用重组SOX5蛋白进行功能分析，揭示其在调控哺乳动物大脑皮质神经元分层发育中的作用。SOX5通过抑制过早分化的转录程序，确保皮质投射神经元按特定顺序生成。
2. **文献名称**: "The transcription factor SOX5 modulates chondrocyte differentiation through direct interaction with COL2A1 enhancer"
**作者**: Nakashima, K., Lefebvre, V.
**摘要**: 通过重组SOX5蛋白的体外实验，研究证实SOX5直接结合Ⅱ型胶原基因(COL2A1)增强子区域，促进软骨细胞分化和细胞外基质合成，为骨骼发育机制提供依据。
3. **文献名称**: "SOX5 functions as a tumor suppressor in glioblastoma by regulating cell cycle progression"
**作者**: Dong, C., Wang, Q., Li, L.
**摘要**: 该研究在大脑胶质母细胞瘤模型中表达重组SOX5蛋白，发现其通过抑制细胞周期蛋白Cyclin D1表达，阻滞肿瘤细胞G1/S期转换，提示SOX5的抑癌作用。
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(注：上述文献信息为模拟示例，实际引用时建议通过PubMed或Google Scholar核实具体文章。)
**Background of SOX5 Recombinant Protein**
The SOX5 protein, encoded by the *SOX5* gene, belongs to the SOX (SRY-related HMG-box) family of transcription factors, which play pivotal roles in cell fate determination, embryonic development, and tissue-specific regulation. As a member of the SOX D subgroup, SOX5 contains a conserved high-mobility group (HMG) domain that enables sequence-specific DNA binding and chromatin remodeling. It is widely expressed during embryogenesis, particularly in the nervous system, cartilage, and testes, where it regulates key developmental processes such as neurogenesis, chondrocyte differentiation, and spermatogenesis.
SOX5 functions as a transcriptional regulator, often acting in synergy or competition with other SOX proteins (e.g., SOX6. SOX9) to modulate target gene expression. In the brain, it is critical for cortical neuron migration and layer formation, while in chondrogenesis, it collaborates with SOX9 to drive cartilage matrix synthesis. Dysregulation of SOX5 has been linked to neurodevelopmental disorders (e.g., autism, intellectual disability), skeletal malformations, and cancers, underscoring its biomedical significance.
Recombinant SOX5 protein is engineered for *in vitro* and *in vivo* studies to dissect its molecular mechanisms. Produced via heterologous expression systems (e.g., *E. coli*, mammalian cells), it retains DNA-binding activity and functional domains, enabling applications such as electrophoretic mobility shift assays (EMSAs), protein interaction studies, and cell differentiation assays. Purification tags (e.g., His, GST) are often incorporated to facilitate isolation and detection.
Research utilizing SOX5 recombinant protein has advanced understanding of its role in developmental pathways and disease, offering potential for therapeutic targeting in conditions associated with SOX5 dysfunction.
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