| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | ITSN2 |
| Uniprot No | Q9NZM3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-1697aa |
| 氨基酸序列 | MMAQFPTAMNGGPNMWAITSEERTKHDRQFDNLKPSGGYITGDQARNFFLQSGLPAPVLAEIWALSDLNKDGKMDQQEFSIAMKLIKLKLQGQQLPVVLPPIMKQPPMFSPLISARFGMGSMPNLSIPQPLPPAAPITSLSSATSGTNLPPLMMPTPLVPSVSTSSLPNGTASLIQPLPIPYSSSTLPHGSSYSLMMGGFGGASIQKAQSLIDLGSSSSTSSTASLSGNSPKTGTSEWAVPQPTRLKYRQKFNTLDKSMSGYLSGFQARNALLQSNLSQTQLATIWTLADVDGDGQLKAEEFILAMHLTDMAKAGQPLPLTLPPELVPPSFRGGKQIDSINGTLPSYQKMQEEEPQKKLPVTFEDKRKANYERGNMELEKRRQALMEQQQREAERKAQKEKEEWERKQRELQEQEWKKQLELEKRLEKQRELERQREEERRKDIERREAAKQELERQRRLEWERIRRQELLNQKNREQEEIVRLNSKKKNLHLELEALNGKHQQISGRLQDVRLKKQTQKTELEVLDKQCDLEIMEIKQLQQELQEYQNKLIYLVPEKQLLNERIKNMQFSNTPDSGVSLLHKKSLEKEELCQRLKEQLDALEKETASKLSEMDSFNNQLKCGNMDDSVLQCLLSLLSCLNNLFLLLKELRETYNTQQLALEQLYKIKRDKLKEIERKRLELMQKKKLEDEAARKAKQGKENLWKENLRKEEEEKQKRLQEEKTQEKIQEEERKAEEKQRKDKDTLKAEEKKRETASVLVNYRALYPFEARNHDEMSFNSGDIIQVDEKTVGEPGWLYGSFQGNFGWFPCNYVEKMPSSENEKAVSPKKALLPPTVSLSATSTSSEPLSSNQPASVTDYQNVSFSNLTVNTSWQKKSAFTRTVSPGSVSPIHGQGQVVENLKAQALCSWTAKKDNHLNFSKHDIITVLEQQENWWFGEVHGGRGWFPKSYVKIIPGSEVKREEPEALYAAVNKKPTSAAYSVGEEYIALYPYSSVEPGDLTFTEGEEILVTQKDGEWWTGSIGDRSGIFPSNYVKPKDQESFGSASKSGASNKKPEIAQVTSAYVASGSEQLSLAPGQLILILKKNTSGWWQGELQARGKKRQKGWFPASHVKLLGPSSERATPAFHPVCQVIAMYDYAANNEDELSFSKGQLINVMNKDDPDWWQGEINGVTGLFPSNYVKMTTDSDPSQQWCADLQTLDTMQPIERKRQGYIHELIQTEERYMADLQLVVEVFQKRMAESGFLTEGEMALIFVNWKELIMSNTKLLKALRVRKKTGGEKMPVQMIGDILAAELSHMQAYIRFCSCQLNGAALLQQKTDEDTDFKEFLKKLASDPRCKGMPLSSFLLKPMQRITRYPLLIRSILENTPESHADHSSLKLALERAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLAEQLIFNSLTNCLGPRKLLHSGKLYKTKSNKELHGFLFNDFLLLTYMVKQFAVSSGSEKLFSSKSNAQFKMYKTPIFLNEVLVKLPTDPSSDEPVFHISHIDRVYTLRTDNINERTAWVQKIKAASEQYIDTEKKKREKAYQARSQKTSGIGRLMVHVIEATELKACKPNGKSNPYCEISMGSQSYTTRTIQDTLNPKWNFNCQFFIKDLYQDVLCLTLFDRDQFSPDDFLGRTEIPVAKIRTEQESKGPMTRRLLLHEVPTGEVWVRFDLQLFEQKTLL |
| 预测分子量 | 193 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于ITSN2重组蛋白研究的示例参考文献(注：以下为假设性示例，具体文献需根据实际数据库检索确认)：
1. **《Expression and functional characterization of recombinant ITSN2 in neuronal endocytosis》**
- 作者：Smith J. et al.
- 摘要：本研究成功在大肠杆菌中表达了重组ITSN2蛋白，并通过体外实验证明其通过EH结构域与内吞相关蛋白相互作用，调控神经细胞突触囊泡的循环过程。
2. **《Structural analysis of ITSN2 SH3 domains and their role in Cdc42 signaling》**
- 作者：Lee H. & Garcia-Mata R.
- 摘要：利用重组ITSN2的SH3结构域蛋白，解析了其与RhoGTP酶Cdc42的相互作用机制，揭示了ITSN2在细胞骨架重组和肿瘤转移中的潜在功能。
3. **《Optimization of ITSN2 recombinant protein purification for in vitro binding assays》**
- 作者：Chen L. et al.
- 摘要：开发了一种基于亲和层析的高效纯化方案，用于获得高纯度ITSN2重组蛋白，并验证其在体外结合实验中对EGFR内吞途径的关键调控作用。
4. **《ITSN2 isoforms: Differential expression and functional divergence in cancer cells》**
- 作者：Wang Y. et al.
- 摘要：通过构建长短两种重组ITSN2异构体，发现长异构体(ITSN2-L)通过调控MAPK信号通路促进肺癌细胞迁移，而短异构体(ITSN2-S)具有抑制作用。
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**注**：实际文献需通过PubMed、Web of Science等平台以关键词"ITSN2 recombinant"或"Intersectin-2 protein expression"检索确认。部分研究可能聚焦于ITSN2的相互作用组学或疾病相关功能。
**Background of ITSN2 Recombinant Protein**
Intersectin-2 (ITSN2) is a member of the intersectin family of scaffold proteins, which play pivotal roles in regulating endocytosis, intracellular trafficking, and signal transduction. ITSN2 is encoded by the *ITSN2* gene and exists as multiple splice variants, including short (ITSN2-S) and long (ITSN2-L) isoforms. These isoforms differ in domain composition, with ITSN2-L containing additional domains such as DH, PH, and C2. which are involved in guanine nucleotide exchange and membrane interactions. Structurally, ITSN2 features EH (Eps15 homology) domains that bind to proteins with NPF motifs, as well as SH3 (Src homology 3) domains that mediate interactions with proline-rich regions of partner proteins, enabling coordination of clathrin-mediated endocytosis and cytoskeletal dynamics.
ITSN2 is ubiquitously expressed but shows enriched expression in neuronal tissues, where it regulates synaptic vesicle recycling and receptor trafficking. Dysregulation of ITSN2 has been linked to neurological disorders, including Alzheimer’s disease, and cancer, where it influences cell proliferation and metastasis. Recombinant ITSN2 proteins are engineered to facilitate *in vitro* and *in vivo* studies of these mechanisms. Produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant ITSN2 retains functional domains, enabling researchers to probe protein interactions, enzymatic activities, or cellular pathways. Its applications span structural studies, drug discovery, and functional assays to dissect ITSN2’s role in health and disease. Unlike ITSN1. which has distinct tissue-specific roles, ITSN2’s unique domains and interactions make it a critical target for understanding cellular communication networks.
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