| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHgA10 |
| Uniprot No | Q9Y5H3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-936aa |
| 氨基酸序列 | MAAQRNRSKESKDCSGLVLLCLFFGIPWEAGARQISYSIPEELEKGSFVGNISKDLGLAPRELAERGVRIVSRGRTQLFSLNPRSGSLITAGRIDREELCAQSARCVVSFNILVEDRVKLFGIEIEVTDINDNAPKFQAENLDVKINENVAAGMRFPLPEAIDPDVGVNSLQSYQLSPNKHFSLRVQSRANGVKYPELVLEHSLDREEEAIHHLVLTASDGGDPLRSGTVLVSVTVFDANDNAPVFTLPEYRVSVPENLPVGTQLLTVTATDRDEGANGEVTYSFRKLPDTQLLKFQLNKYTGEIKISENLDYEETGFYEIEIQAEDGGAYLATAKVLITVEDVNDNSPELTITSLFSPVTEDSPLGTVVALLNVHDLDSEQNGQVTCSILAYLPFKLEKSIDSYYRLVIHRALDREQVSSYNITVTATDGGSPPLSTEAHFMLQVADINDNPPTFSQVSYFTYIPENNARGASIFSVTALDPDSKENAQIIYSLAEDTIQGVPLSSYISINSDTGVLYALRSFDYEQFHELQMQVTASDSGDPPLSSNVSLSLFVLDQNDNAPEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYRLLKASEPGLFAVGEHTGEVRTARALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADSIPQVLADLGSFESPANSETSDLTLYLVVAVAAVSCVFLAFVIVLLAHRLRRWHKSRLLQASGGGLTGVSGSHFVGVDGVRAFLQTYSHEVSLTADSRKSHLIFPQPNYADTLISQESCEKNDPLSLLDDSKFPIEDTPLVPQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 预测分子量 | 101 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是假设性的参考文献示例，供参考。由于PCDHGA10重组蛋白的研究可能较为专业，具体文献需通过学术数据库获取：
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**1. 文献名称**: "Recombinant Expression and Functional Characterization of Protocadherin-γA10 in Neuronal Development"
**作者**: Smith J, et al.
**摘要**: 本研究利用哺乳动物表达系统成功表达了PCDHγA10重组蛋白，证实其在神经元突触形成中的关键作用，通过体外实验证明其介导细胞间粘附并影响轴突导向。
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**2. 文献名称**: "Structural Insights into PCDHGA10 Recombinant Protein via Cryo-EM Analysis"
**作者**: Lee S, et al.
**摘要**: 报道了PCDHγA10重组蛋白的冷冻电镜结构解析，揭示了其胞外结构域的特异性钙离子结合位点，为理解原钙粘蛋白家族的结构-功能关系提供依据。
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**3. 文献名称**: "PCDHGA10 Recombinant Protein Suppresses Tumor Metastasis by Modulating Cell Adhesion"
**作者**: Zhang Y, et al.
**摘要**: 通过大肠杆菌表达系统制备PCDHγA10重组蛋白，发现其能抑制癌细胞迁移并增强同型细胞粘附，提示其作为肿瘤治疗潜在靶点。
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**4. 文献名称**: "Optimization of PCDHGA10 Recombinant Protein Purification for Immunoassay Development"
**作者**: Brown K, et al.
**摘要**: 开发了基于亲和层析的高效纯化方案，获得高纯度PCDHγA10重组蛋白，并验证其在ELISA检测中的临床应用潜力。
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**说明**：以上文献为模拟示例，实际研究需查阅PubMed、Web of Science等平台，结合关键词“PCDHGA10 recombinant protein”或“protocadherin gamma A10 expression”检索。建议关注近年文献以获取最新进展。
PCDHgA10 (Protocadherin gamma subfamily A, member 10) is a member of the protocadherin γ gene cluster, a subgroup within the cadherin superfamily known for mediating calcium-dependent cell-cell adhesion. This cluster consists of 22 tandemly arranged genes (A1–A10. B1–B7. and C1–C5) that are predominantly expressed in the vertebrate nervous system. PCDHgA10 encodes a single-pass transmembrane protein characterized by an extracellular domain with six cadherin-like repeats, a transmembrane domain, and a conserved cytoplasmic tail. Unlike classical cadherins, protocadherins exhibit combinatorial diversity through alternative promoter usage and splicing, contributing to neuronal identity and synaptic specificity during brain development.
The PCDHγ family plays critical roles in neuronal circuit assembly, axon guidance, dendrite self-avoidance, and synapse formation. PCDHgA10. as a member of the "A" subgroup, is proposed to function in establishing precise neuronal connectivity through homophilic or heterophilic interactions. Its expression is tightly regulated during neurodevelopment, with studies linking defects in protocadherin genes to neurodevelopmental disorders, including autism spectrum disorders and schizophrenia.
Recombinant PCDHgA10 protein is typically produced in mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications. The purified protein, often fused with tags like Fc or His for detection and purification, serves as a tool to study cell adhesion mechanisms, receptor-ligand interactions, and signaling pathways in neuronal cultures or in vitro assays. Researchers also utilize it to generate specific antibodies or screen for small molecules targeting protocadherin-mediated processes. Current investigations focus on elucidating its role in synaptic plasticity and its potential as a biomarker or therapeutic target in neurological diseases.
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