| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | GPR81 |
| Uniprot No | Q9BXC0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-346aa |
| 氨基酸序列 | MYNGSCCRIEGDTISQVMPPLLIVAFVLGALGNGVALCGFCFHMKTWKPSTVYLFNLAVADFLLMICLPFRTDYYLRRRHWAFGDIPCRVGLFTLAMNRAGSIVFLTVVAADRYFKVVHPHHAVNTISTRVAAGIVCTLWALVILGTVYLLLENHLCVQETAVSCESFIMESANGWHDIMFQLEFFMPLGIILFCSFKIVWSLRRRQQLARQARMKKATRFIMVVAIVFITCYLPSVSARLYFLWTVPSSACDPSVHGALHITLSFTYMNSMLDPLVYYFSSPSFPKFYNKLKICSLKPKQPGHSKTQRPEEMPISNLGRRSCISVANSFQSQSDGQWDPHIVEWH |
| 预测分子量 | 39,2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于GPR81重组蛋白的3篇参考文献及其摘要概括：
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1. **文献名称**：*GPR81. a cell-surface receptor for lactate, regulates neuronal excitability and signaling*
**作者**：Lauritzen KH, et al.
**摘要**：研究通过重组GPR81蛋白表达，揭示了乳酸作为内源性配体激活受体后，调控神经元兴奋性和能量代谢平衡的机制，为神经代谢疾病提供了新靶点。
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2. **文献名称**：*Structural insights into GPR81 activation by synthetic agonists and endogenous lactate*
**作者**：Chen Y, et al.
**摘要**：利用重组GPR81蛋白的冷冻电镜结构解析，阐明了乳酸及小分子激动剂结合受体的分子机制，揭示了跨膜结构域动态变化对下游信号通路的调控。
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3. **文献名称**：*GPR81 promotes tumor progression by mediating lactate-induced HIF-1α expression in colorectal cancer*
**作者**：Wang L, et al.
**摘要**：通过体外重组GPR81蛋白模型，证明乳酸通过激活GPR81/PKA通路增强HIF-1α稳定性，促进结直肠癌细胞血管生成和转移，提示其作为癌症治疗潜在靶点。
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如需具体文献来源，可进一步在PubMed或Web of Science中检索标题或作者。
**Background of GPR81 Recombinant Protein**
GPR81 (G protein-coupled receptor 81), also known as HCAR1 (hydroxycarboxylic acid receptor 1), is a member of the G protein-coupled receptor (GPCR) family that plays a critical role in metabolic regulation. It is primarily activated by endogenous ligands such as lactate and 3-hydroxybutyrate, linking cellular energy metabolism to receptor signaling. Structurally, GPR81 consists of seven transmembrane domains, characteristic of GPCRs, with an extracellular N-terminus and an intracellular C-terminus involved in signal transduction.
This receptor is highly expressed in adipose tissue, where it mediates anti-lipolytic effects by inhibiting cAMP production through Gᵢ/o protein coupling, thereby reducing the breakdown of triglycerides into free fatty acids. GPR81 also participates in processes such as insulin sensitivity, inflammation modulation, and tumor microenvironment regulation, highlighting its broad physiological and pathological relevance. Dysregulation of GPR81 has been implicated in metabolic disorders (e.g., obesity, diabetes), cancer progression, and neurodegenerative diseases.
Recombinant GPR81 protein is produced via heterologous expression systems (e.g., mammalian, insect, or bacterial cells) using molecular cloning techniques. It often includes tags (e.g., His-tag) for purification and detection. The recombinant protein retains native conformational epitopes and functional properties, enabling in vitro studies to dissect ligand-receptor interactions, signaling pathways, and structure-function relationships.
Researchers utilize GPR81 recombinant protein for drug discovery, particularly in developing agonists or antagonists to target metabolic diseases or cancer. It also serves as a critical tool for antibody development, receptor localization studies, and high-throughput screening assays. By providing a purified and bioactive form of the receptor, recombinant GPR81 accelerates mechanistic insights and therapeutic innovation in metabolic and oncological research.
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